Host bone microstructure for enhanced resistance to bacterial infections

Watanabe, Ryota; Matsugaki, Aira; Gokcekaya, Ozkan; Ozasa, Ryosuke; Matsumoto, Takuya; Takahashi, Hiroyuki; Yasui, Hidekazu; Nakano, Takayoshi

doi:https://doi.org/10.1016/j.bioadv.2023.213633

Host bone microstructure for enhanced resistance to bacterial infections

Watanabe, Ryota; Matsugaki, Aira; Gokcekaya, Ozkan et al.

Biomaterials Advances, 2023, 154, 213633

Number of Access:1,033（2025-07-27 21:13 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93144

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Title	Host bone microstructure for enhanced resistance to bacterial infections
Creator	CreatorName Watanabe, Ryota
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	ORCID 0000-0002-9258-858X 0000-0002-9258-858X Scopus Author ID 37051975500 37051975500 NRID 1000050988160 1000050988160 CreatorName Gokcekaya, Ozkan
	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000040324793 1000040324793 CreatorName Matsumoto, Takuya
	CreatorName Takahashi, Hiroyuki
	CreatorName Yasui, Hidekazu
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Antimicrobial activity
	Bone matrix anisotropy
	Laser powder bed fusion
	Postoperative infection
	Surface topography
	Titanium alloy
Description	Watanabe R., Matsugaki A., Gokcekaya O., et al. Host bone microstructure for enhanced resistance to bacterial infections. Biomaterials Advances 154, 213633 (2023); https://doi.org/10.1016/j.bioadv.2023.213633.
Abstract	Postoperative bacterial infection is a serious complication of orthopedic surgery. Not only infections that develop in the first few weeks after surgery but also late infections that develop years after surgery are serious problems. However, the relationship between host bone and infection activation has not yet been explored. Here, we report a novel association between host bone collagen/apatite microstructure and bacterial infection. The bone-mimetic-oriented micro-organized matrix structure was obtained by prolonged controlled cell alignment using a grooved-structured biomedical titanium alloy. Surprisingly, we have discovered that highly aligned osteoblasts have a potent inhibitory effect on Escherichia coli adhesion. Additionally, the oriented collagen/apatite micro-organization of the bone matrix showed excellent antibacterial resistance against Escherichia coli. The proposed mechanism for realizing the antimicrobial activity of the micro-organized bone matrix is by the controlled secretion of the antimicrobial peptides, including β-defensin 2 and β-defensin 3, from the highly aligned osteoblasts. Our findings contribute to the development of anti-infective strategies for orthopedic surgeries. The recovery of the intrinsically ordered bone matrix organization provides superior antibacterial resistance after surgery.
Publisher	Elsevier Ltd
Source Title	Biomaterials Advances
Volume (Issue)	154
Page	213633
Date of Issued	2023-11-01
Language	English
Handle URL	https://hdl.handle.net/11094/93144
eISSN	27729508
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bioadv.2023.213633
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biomaterials Advances. 2023, 154, p. 213633
DC.title	Host bone microstructure for enhanced resistance to bacterial infections
DC.creator	Watanabe, Ryota
	Matsugaki, Aira
	Gokcekaya, Ozkan
	Ozasa, Ryosuke
	Matsumoto, Takuya
	Takahashi, Hiroyuki
	Yasui, Hidekazu
	Nakano, Takayoshi
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-11-01
DC.identifier	https://doi.org/10.1016/j.bioadv.2023.213633
DC.subject	Antimicrobial activity
	Bone matrix anisotropy
	Laser powder bed fusion
	Postoperative infection
	Surface topography
	Titanium alloy
DC.description	Watanabe R., Matsugaki A., Gokcekaya O., et al. Host bone microstructure for enhanced resistance to bacterial infections. Biomaterials Advances 154, 213633 (2023); https://doi.org/10.1016/j.bioadv.2023.213633.
DCTERMS.abstract	Postoperative bacterial infection is a serious complication of orthopedic surgery. Not only infections that develop in the first few weeks after surgery but also late infections that develop years after surgery are serious problems. However, the relationship between host bone and infection activation has not yet been explored. Here, we report a novel association between host bone collagen/apatite microstructure and bacterial infection. The bone-mimetic-oriented micro-organized matrix structure was obtained by prolonged controlled cell alignment using a grooved-structured biomedical titanium alloy. Surprisingly, we have discovered that highly aligned osteoblasts have a potent inhibitory effect on Escherichia coli adhesion. Additionally, the oriented collagen/apatite micro-organization of the bone matrix showed excellent antibacterial resistance against Escherichia coli. The proposed mechanism for realizing the antimicrobial activity of the micro-organized bone matrix is by the controlled secretion of the antimicrobial peptides, including β-defensin 2 and β-defensin 3, from the highly aligned osteoblasts. Our findings contribute to the development of anti-infective strategies for orthopedic surgeries. The recovery of the intrinsically ordered bone matrix organization provides superior antibacterial resistance after surgery.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93144
citation_title	Host bone microstructure for enhanced resistance to bacterial infections
citation_author	Watanabe, Ryota
	Matsugaki, Aira
	Gokcekaya, Ozkan
	Ozasa, Ryosuke
	Matsumoto, Takuya
	Takahashi, Hiroyuki
	Yasui, Hidekazu
	Nakano, Takayoshi
citation_publisher	Elsevier Ltd
citation_language	English
citation_date	2023-11-01
citation_journal_title	Biomaterials Advances
citation_volume	154
citation_firstpage	213633
citation_doi	https://doi.org/10.1016/j.bioadv.2023.213633
citation_public_url	https://hdl.handle.net/11094/93144

Host bone microstructure for enhanced resistance to bacterial infections

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