Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft

Matsugaki, Aira; Ito, Manabu; Kobayashi, Yoshiya; Matsuzaka, Tadaaki; Ozasa, Ryosuke; Ishimoto, Takuya; Takahashi, Hiroyuki; Watanabe, Ryota; Inoue, Takayuki; Yokota, Katsuhiko; Nakashima, Yoshio; Kaito, Takashi; Okada, Seiji; Hanawa, Takao; Matsuyama, Yukihiro; Matsumoto, Morio; Taneichi, Hiroshi; Nakano, Takayoshi

doi:https://doi.org/10.1016/j.spinee.2022.12.011

Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft

Matsugaki, Aira; Ito, Manabu; Kobayashi, Yoshiya et al.

Spine Journal, 2023, 23(4), 609-620

Number of Access:2,294（2025-07-18 09:54 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93145

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Title	Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft
Creator	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	CreatorName Ito, Manabu
	CreatorName Kobayashi, Yoshiya
	CreatorName Matsuzaka, Tadaaki
	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	CreatorName Takahashi, Hiroyuki
	CreatorName Watanabe, Ryota
	CreatorName Inoue, Takayuki
	CreatorName Yokota, Katsuhiko
	CreatorName Nakashima, Yoshio
	NRID 1000070623982 1000070623982 ORCID 0000-0003-4882-2997 0000-0003-4882-2997 researchmap permalink 911_0 911_0 Scopus Author ID 25824322100 25824322100 CreatorName Kaito, Takashi
	NRID 1000030448435 1000030448435 ORCID 0000-0002-5107-8209 0000-0002-5107-8209 researchmap permalink okadaseiji okadaseiji Scopus Author ID 35079139100 35079139100 CreatorName Okada, Seiji
	NRID 1000090142736 1000090142736 CreatorName Hanawa, Takao
	NRID 1000020312316 1000020312316 CreatorName Matsuyama, Yukihiro
	NRID 1000040209656 1000040209656 CreatorName Matsumoto, Morio
	NRID 1000010261299 1000010261299 CreatorName Taneichi, Hiroshi
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Bone quality
	Collagen and apatite orientation
	Intervertebral spacer
	Push-out strength
	Spinal fusion
	Through-pore grooved surface structure
Description	Matsugaki A., Ito M., Kobayashi Y., et al. Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft. Spine Journal 23, 609 (2023); https://doi.org/10.1016/j.spinee.2022.12.011.
Abstract	BACKGROUND CONTEXT: Although autologous bone grafting is widely considered as an ideal source for interbody fusion, it still carries a risk of nonunion. The influence of the intervertebral device should not be overlooked. Requirements for artificial spinal devices are to join the vertebrae together and recover the original function of the spine rapidly. Ordered mineralization of apatite crystals on collagen accelerates bone functionalization during the healing process. Particularly, the stable spinal function requires the ingrowth of an ordered collagen and apatite matrix which mimics the intact intervertebral microstructure. This collagen and apatite ordering is imperative for functional bone regeneration, which has not been achieved using classical autologous grafting. PURPOSE: We developed an intervertebral body device to achieve high stability between the host bone and synthesized bone by controlling the ordered collagen and apatite microstructure. STUDY DESIGN: This was an in vivo animal study. METHODS: Intervertebral spacers with a through-pore grooved surface structure, referred to as a honeycomb tree structure, were produced using metal 3D printing. These spacers were implanted into normal sheep at the L2–L3 or L4–L5 disc levels. As a control group, grafting autologous bone was embedded. The mechanical integrity of the spacer/bone interface was evaluated through push-out tests. RESULTS: The spacer with honeycomb tree structure induced anisotropic trabecular bone growth with textured collagen and apatite orientation in the through-pore and groove directions. The push-out load of the spacer was significantly higher than that of the conventional autologous graft spacer. Moreover, the load was significantly correlated with the anisotropic texture of the newly formed bone matrix. CONCLUSIONS: The developed intervertebral spacer guided the regenerated bone matrix orientation of collagen and apatite, resulting in greater strength at the spacer/host bone interface than that obtained using a conventional gold-standard autologous bone graft. CLINICAL SIGNIFICANCE: Our results provide a foundation for designing future spacers for interbody fusion in human.
Publisher	Elsevier Inc.
Source Title	Spine Journal
Volume (Issue)	23(4)
Page	609-620
Date of Issued	2023-04-01
Language	English
Handle URL	https://hdl.handle.net/11094/93145
PISSN	15299430
eISSN	18781632
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.spinee.2022.12.011
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Spine Journal. 2023, 23(4), p. 609-620
DC.title	Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft
DC.creator	Matsugaki, Aira
	Ito, Manabu
	Kobayashi, Yoshiya
	Matsuzaka, Tadaaki
	Ozasa, Ryosuke
	Ishimoto, Takuya
	Takahashi, Hiroyuki
	Watanabe, Ryota
	Inoue, Takayuki
	Yokota, Katsuhiko
	Nakashima, Yoshio
	Kaito, Takashi
	Okada, Seiji
	Hanawa, Takao
	Matsuyama, Yukihiro
	Matsumoto, Morio
	Taneichi, Hiroshi
	Nakano, Takayoshi
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-04-01
DC.identifier	https://doi.org/10.1016/j.spinee.2022.12.011
DC.subject	Bone quality
	Collagen and apatite orientation
	Intervertebral spacer
	Push-out strength
	Spinal fusion
	Through-pore grooved surface structure
DC.description	Matsugaki A., Ito M., Kobayashi Y., et al. Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft. Spine Journal 23, 609 (2023); https://doi.org/10.1016/j.spinee.2022.12.011.
DCTERMS.abstract	BACKGROUND CONTEXT: Although autologous bone grafting is widely considered as an ideal source for interbody fusion, it still carries a risk of nonunion. The influence of the intervertebral device should not be overlooked. Requirements for artificial spinal devices are to join the vertebrae together and recover the original function of the spine rapidly. Ordered mineralization of apatite crystals on collagen accelerates bone functionalization during the healing process. Particularly, the stable spinal function requires the ingrowth of an ordered collagen and apatite matrix which mimics the intact intervertebral microstructure. This collagen and apatite ordering is imperative for functional bone regeneration, which has not been achieved using classical autologous grafting. PURPOSE: We developed an intervertebral body device to achieve high stability between the host bone and synthesized bone by controlling the ordered collagen and apatite microstructure. STUDY DESIGN: This was an in vivo animal study. METHODS: Intervertebral spacers with a through-pore grooved surface structure, referred to as a honeycomb tree structure, were produced using metal 3D printing. These spacers were implanted into normal sheep at the L2–L3 or L4–L5 disc levels. As a control group, grafting autologous bone was embedded. The mechanical integrity of the spacer/bone interface was evaluated through push-out tests. RESULTS: The spacer with honeycomb tree structure induced anisotropic trabecular bone growth with textured collagen and apatite orientation in the through-pore and groove directions. The push-out load of the spacer was significantly higher than that of the conventional autologous graft spacer. Moreover, the load was significantly correlated with the anisotropic texture of the newly formed bone matrix. CONCLUSIONS: The developed intervertebral spacer guided the regenerated bone matrix orientation of collagen and apatite, resulting in greater strength at the spacer/host bone interface than that obtained using a conventional gold-standard autologous bone graft. CLINICAL SIGNIFICANCE: Our results provide a foundation for designing future spacers for interbody fusion in human.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93145
citation_title	Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft
citation_author	Matsugaki, Aira
	Ito, Manabu
	Kobayashi, Yoshiya
	Matsuzaka, Tadaaki
	Ozasa, Ryosuke
	Ishimoto, Takuya
	Takahashi, Hiroyuki
	Watanabe, Ryota
	Inoue, Takayuki
	Yokota, Katsuhiko
	Nakashima, Yoshio
	Kaito, Takashi
	Okada, Seiji
	Hanawa, Takao
	Matsuyama, Yukihiro
	Matsumoto, Morio
	Taneichi, Hiroshi
	Nakano, Takayoshi
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2023-04-01
citation_journal_title	Spine Journal
citation_volume	23
citation_issue	4
citation_firstpage	609
citation_lastpage	620
citation_doi	https://doi.org/10.1016/j.spinee.2022.12.011
citation_public_url	https://hdl.handle.net/11094/93145

Innovative design of bone quality-targeted intervertebral spacer: accelerated functional fusion guiding oriented collagen and apatite microstructure without autologous bone graft

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