Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo

Uenaka, Maki; Yamashita, Erika; Kikuta, Junichi; Morimoto, Akito; Ao, Tomoka; Mizuno, Hiroki; Furuya, Masayuki; Hasegawa, Tetsuo; Tsukazaki, Hiroyuki; Sudo, Takao; Nishikawa, Keizo; Okuzaki, Daisuke; Motooka, Daisuke; Kosaka, Nobuyoshi; Sugihara, Fuminori; Boettger, Thomas; Braun, Thomas; Ochiya, Takahiro; Ishii, Masaru

doi:https://doi.org/10.1038/s41467-022-28673-2

Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo

Uenaka, Maki; Yamashita, Erika; Kikuta, Junichi et al.

Nature Communications, 2022, 13(1), 1066

Number of Access:2,940（2025-08-18 14:22 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93153

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
NatCommun_13_1	pdf	None	5.99 MB	115	2023.11.17

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo
Creator	CreatorName Uenaka, Maki
	NRID 1000010880106 1000010880106 ORCID 0000-0002-3973-9529 0000-0002-3973-9529 researchmap permalink erika-ys erika-ys CreatorName Yamashita, Erika
	NRID 1000060710069 1000060710069 researchmap permalink jkikuta jkikuta Scopus Author ID 36637603800 36637603800 CreatorName Kikuta, Junichi
	NRID 1000010881740 1000010881740 Scopus Author ID 57200327474 57200327474 CreatorName Morimoto, Akito
	NRID 1000090880865 1000090880865 researchmap permalink tomoka_ao1203 tomoka_ao1203 CreatorName Ao, Tomoka
	NRID 1000090707655 1000090707655 CreatorName Mizuno, Hiroki
	NRID 1000020987466 1000020987466 researchmap permalink furuya1019 furuya1019 CreatorName Furuya, Masayuki
	NRID 1000030773048 1000030773048 CreatorName Hasegawa, Tetsuo
	CreatorName Tsukazaki, Hiroyuki
	NRID 1000080631184 1000080631184 researchmap permalink kik kik Scopus Author ID 36497716100 36497716100 CreatorName Sudo, Takao
	NRID 1000030516290 1000030516290 CreatorName Nishikawa, Keizo
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 CreatorName Okuzaki, Daisuke
	NRID 1000010636830 1000010636830 researchmap permalink daisukem daisukem Scopus Author ID 35315737900 35315737900 CreatorName Motooka, Daisuke
	CreatorName Kosaka, Nobuyoshi
	NRID 1000050381645 1000050381645 researchmap permalink sugiharaf sugiharaf Scopus Author ID 12809512100 12809512100 CreatorName Sugihara, Fuminori
	CreatorName Boettger, Thomas
	CreatorName Braun, Thomas
	NRID 1000060192530 1000060192530 CreatorName Ochiya, Takahiro
	NRID 1000010324758 1000010324758 researchmap permalink read0076684 read0076684 Scopus Author ID 35074093500 35074093500 CreatorName Ishii, Masaru
Description	Uenaka M., Yamashita E., Kikuta J., et al. Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo. Nature Communications 13, 1066 (2022); https://doi.org/10.1038/s41467-022-28673-2.
Abstract	Bone metabolism is regulated by the cooperative activity between bone-forming osteoblasts and bone-resorbing osteoclasts. However, the mechanisms mediating the switch between the osteoblastic and osteoclastic phases have not been fully elucidated. Here, we identify a specific subset of mature osteoblast-derived extracellular vesicles that inhibit bone formation and enhance osteoclastogenesis. Intravital imaging reveals that mature osteoblasts secrete and capture extracellular vesicles, referred to as small osteoblast vesicles (SOVs). Co-culture experiments demonstrate that SOVs suppress osteoblast differentiation and enhance the expression of receptor activator of NF-κB ligand, thereby inducing osteoclast differentiation. We also elucidate that the SOV-enriched microRNA miR-143 inhibits Runt-related transcription factor 2, a master regulator of osteoblastogenesis, by targeting the mRNA expression of its dimerization partner, core-binding factor β. In summary, we identify SOVs as a mode of cell-to-cell communication, controlling the dynamic transition from bone-forming to bone-resorbing phases in vivo.
Publisher	Nature Research
Source Title	Nature Communications
Volume (Issue)	13(1)
Page	1066
Date of Issued	2022-02-24
Language	English
Handle URL	https://hdl.handle.net/11094/93153
eISSN	20411723
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s41467-022-28673-2
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Nature Communications. 2022, 13(1), p. 1066
DC.title	Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo
DC.creator	Uenaka, Maki
	Yamashita, Erika
	Kikuta, Junichi
	Morimoto, Akito
	Ao, Tomoka
	Mizuno, Hiroki
	Furuya, Masayuki
	Hasegawa, Tetsuo
	Tsukazaki, Hiroyuki
	Sudo, Takao
	Nishikawa, Keizo
	Okuzaki, Daisuke
	Motooka, Daisuke
	Kosaka, Nobuyoshi
	Sugihara, Fuminori
	Boettger, Thomas
	Braun, Thomas
	Ochiya, Takahiro
	Ishii, Masaru
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-02-24
DC.identifier	https://doi.org/10.1038/s41467-022-28673-2
DC.description	Uenaka M., Yamashita E., Kikuta J., et al. Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo. Nature Communications 13, 1066 (2022); https://doi.org/10.1038/s41467-022-28673-2.
DCTERMS.abstract	Bone metabolism is regulated by the cooperative activity between bone-forming osteoblasts and bone-resorbing osteoclasts. However, the mechanisms mediating the switch between the osteoblastic and osteoclastic phases have not been fully elucidated. Here, we identify a specific subset of mature osteoblast-derived extracellular vesicles that inhibit bone formation and enhance osteoclastogenesis. Intravital imaging reveals that mature osteoblasts secrete and capture extracellular vesicles, referred to as small osteoblast vesicles (SOVs). Co-culture experiments demonstrate that SOVs suppress osteoblast differentiation and enhance the expression of receptor activator of NF-κB ligand, thereby inducing osteoclast differentiation. We also elucidate that the SOV-enriched microRNA miR-143 inhibits Runt-related transcription factor 2, a master regulator of osteoblastogenesis, by targeting the mRNA expression of its dimerization partner, core-binding factor β. In summary, we identify SOVs as a mode of cell-to-cell communication, controlling the dynamic transition from bone-forming to bone-resorbing phases in vivo.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93153
citation_title	Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo
citation_author	Uenaka, Maki
	Yamashita, Erika
	Kikuta, Junichi
	Morimoto, Akito
	Ao, Tomoka
	Mizuno, Hiroki
	Furuya, Masayuki
	Hasegawa, Tetsuo
	Tsukazaki, Hiroyuki
	Sudo, Takao
	Nishikawa, Keizo
	Okuzaki, Daisuke
	Motooka, Daisuke
	Kosaka, Nobuyoshi
	Sugihara, Fuminori
	Boettger, Thomas
	Braun, Thomas
	Ochiya, Takahiro
	Ishii, Masaru
citation_publisher	Nature Research
citation_language	English
citation_date	2022-02-24
citation_journal_title	Nature Communications
citation_volume	13
citation_issue	1
citation_firstpage	1066
citation_doi	https://doi.org/10.1038/s41467-022-28673-2
citation_public_url	https://hdl.handle.net/11094/93153

Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo

Link to primary information

Item Information

Show details