In vivo dynamic analysis of BMP-2-induced ectopic bone formation

Hashimoto, Kunihiko; Kaito, Takashi; Furuya, Masayuki; Seno, Shigeto; Okuzaki, Daisuke; Kikuta, Junichi; Tsukazaki, Hiroyuki; Matsuda, Hideo; Yoshikawa, Hideki; Ishii, Masaru

doi:https://doi.org/10.1038/s41598-020-61825-2

In vivo dynamic analysis of BMP-2-induced ectopic bone formation

Hashimoto, Kunihiko; Kaito, Takashi; Furuya, Masayuki et al.

Scientific Reports, 2020, 10(1), 4751

Number of Access:1,774（2025-07-24 08:45 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93167

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Title	In vivo dynamic analysis of BMP-2-induced ectopic bone formation
Creator	CreatorName Hashimoto, Kunihiko
	NRID 1000070623982 1000070623982 ORCID 0000-0003-4882-2997 0000-0003-4882-2997 researchmap permalink 911_0 911_0 Scopus Author ID 25824322100 25824322100 CreatorName Kaito, Takashi
	NRID 1000020987466 1000020987466 researchmap permalink furuya1019 furuya1019 CreatorName Furuya, Masayuki
	NRID 1000030432462 1000030432462 ORCID 0000-0003-3861-6444 0000-0003-3861-6444 researchmap permalink ssenoo ssenoo Scopus Author ID 57214748425 57214748425 CreatorName Seno, Shigeto
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 CreatorName Okuzaki, Daisuke
	NRID 1000060710069 1000060710069 researchmap permalink jkikuta jkikuta Scopus Author ID 36637603800 36637603800 CreatorName Kikuta, Junichi
	CreatorName Tsukazaki, Hiroyuki
	NRID 1000050183950 1000050183950 ORCID 0000-0002-4701-3779 0000-0002-4701-3779 researchmap permalink Hideo_Matsuda Hideo_Matsuda Scopus Author ID 35381793100 35381793100 CreatorName Matsuda, Hideo
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, Hideki
	NRID 1000010324758 1000010324758 researchmap permalink read0076684 read0076684 Scopus Author ID 35074093500 35074093500 CreatorName Ishii, Masaru
Description	Hashimoto K., Kaito T., Furuya M., et al. In vivo dynamic analysis of BMP-2-induced ectopic bone formation. Scientific Reports 10, 4751 (2020); https://doi.org/10.1038/s41598-020-61825-2.
Abstract	Bone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of BMP-2-induced ectopic bone formation. Using multicolor intravital imaging in transgenic mice, we visualized the spatiotemporal process of bone induction, including appearance and motility of osteoblasts and osteoclasts, angiogenesis, collagen-fiber formation, and bone-mineral deposition. Furthermore, we investigated how PTH1-34 affects BMP-2-induced bone formation, which revealed that PTH1-34 administration accelerated differentiation and increased the motility of osteoblasts, whereas it decreased morphological changes in osteoclasts. This is the first report on visualization of the entire process of BMP-2-induced bone formation using intravital imaging techniques, which, we believe, will contribute to our understanding of ectopic bone formation and provide new parameters for evaluating bone-forming activity.
Publisher	Nature Research
Source Title	Scientific Reports
Volume (Issue)	10(1)
Page	4751
Date of Issued	2020-03-16
Language	English
Handle URL	https://hdl.handle.net/11094/93167
eISSN	20452322
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s41598-020-61825-2
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Scientific Reports. 2020, 10(1), p. 4751
DC.title	In vivo dynamic analysis of BMP-2-induced ectopic bone formation
DC.creator	Hashimoto, Kunihiko
	Kaito, Takashi
	Furuya, Masayuki
	Seno, Shigeto
	Okuzaki, Daisuke
	Kikuta, Junichi
	Tsukazaki, Hiroyuki
	Matsuda, Hideo
	Yoshikawa, Hideki
	Ishii, Masaru
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-03-16
DC.identifier	https://doi.org/10.1038/s41598-020-61825-2
DC.description	Hashimoto K., Kaito T., Furuya M., et al. In vivo dynamic analysis of BMP-2-induced ectopic bone formation. Scientific Reports 10, 4751 (2020); https://doi.org/10.1038/s41598-020-61825-2.
DCTERMS.abstract	Bone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of BMP-2-induced ectopic bone formation. Using multicolor intravital imaging in transgenic mice, we visualized the spatiotemporal process of bone induction, including appearance and motility of osteoblasts and osteoclasts, angiogenesis, collagen-fiber formation, and bone-mineral deposition. Furthermore, we investigated how PTH1-34 affects BMP-2-induced bone formation, which revealed that PTH1-34 administration accelerated differentiation and increased the motility of osteoblasts, whereas it decreased morphological changes in osteoclasts. This is the first report on visualization of the entire process of BMP-2-induced bone formation using intravital imaging techniques, which, we believe, will contribute to our understanding of ectopic bone formation and provide new parameters for evaluating bone-forming activity.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93167
citation_title	In vivo dynamic analysis of BMP-2-induced ectopic bone formation
citation_author	Hashimoto, Kunihiko
	Kaito, Takashi
	Furuya, Masayuki
	Seno, Shigeto
	Okuzaki, Daisuke
	Kikuta, Junichi
	Tsukazaki, Hiroyuki
	Matsuda, Hideo
	Yoshikawa, Hideki
	Ishii, Masaru
citation_publisher	Nature Research
citation_language	English
citation_date	2020-03-16
citation_journal_title	Scientific Reports
citation_volume	10
citation_issue	1
citation_firstpage	4751
citation_doi	https://doi.org/10.1038/s41598-020-61825-2
citation_public_url	https://hdl.handle.net/11094/93167

In vivo dynamic analysis of BMP-2-induced ectopic bone formation

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