Imaging the bone-immune cell interaction in bone destruction

Hasegawa, Tetsuo; Kikuta, Junichi; Ishii, Masaru

Frontiers in Immunology, 2019, 10(MAR), 596

Number of Access:494（2025-08-18 12:33 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93172

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
FrontImmunol_10_MAR pdf None 1.21 MB 32 2023.11.21  
Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Imaging the bone-immune cell interaction in bone destruction
Creator
NRID
1000030773048 1000030773048
CreatorName
Hasegawa, Tetsuo
NRID
1000060710069 1000060710069
researchmap permalink
jkikuta jkikuta
Scopus Author ID
36637603800 36637603800
CreatorName
Kikuta, Junichi
NRID
1000010324758 1000010324758
researchmap permalink
read0076684 read0076684
Scopus Author ID
35074093500 35074093500
CreatorName
Ishii, Masaru
Subject
Bone
Cellular dynamics
Intravital imaging
Osteoclast
PH probe
Two-photon microscopy
Description
Hasegawa T., Kikuta J., Ishii M.. Imaging the bone-immune cell interaction in bone destruction. Frontiers in Immunology 10, 596 (2019); https://doi.org/10.3389/fimmu.2019.00596.
Abstract
Bone is a highly dynamic organ that is continuously being remodeled by the reciprocal interactions between bone and immune cells. We have originally established an advanced imaging system for visualizing the in vivo behavior of osteoclasts and their precursors in the bone marrow cavity using two-photon microscopy. Using this system, we found that the blood-enriched lipid mediator, sphingosine-1-phosphate, controlled the migratory behavior of osteoclast precursors. We also developed pH-sensing chemical fluorescent probes to detect localized acidification by bone-resorbing osteoclasts on the bone surface in vivo, and identified two distinct functional states of differentiated osteoclasts, “bone-resorptive” and “non-resorptive.” Here, we summarize our studies on the dynamics and functions of bone and immune cells within the bone marrow. We further discuss how our intravital imaging techniques can be applied to evaluate the mechanisms of action of biological agents in inflammatory bone destruction. Our intravital imaging techniques would be beneficial for studying the cellular dynamics in arthritic inflammation and bone destruction in vivo and would also be useful for evaluating novel therapies in animal models of bone-destroying diseases.
Publisher
Frontiers Media
Source Title
Frontiers in Immunology
Volume (Issue)
10(MAR)
Page
596
Date of Issued
2019-03-26
Language
English
Handle URL
https://hdl.handle.net/11094/93172
eISSN
16643224
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.3389/fimmu.2019.00596
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article