Administration of oxygen ultra-fine bubbles improves nerve dysfunction in a rat sciatic nerve crush injury model

Matsuoka, Hozo; Ebina, Kosuke; Tanaka, Hiroyuki et al.

International Journal of Molecular Sciences, 2018, 19(5), 1395

Number of Access:1,293（2025-09-03 11:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93214

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Title
Administration of oxygen ultra-fine bubbles improves nerve dysfunction in a rat sciatic nerve crush injury model
Creator
CreatorName
Matsuoka, Hozo
NRID
1000070612076 1000070612076
researchmap permalink
k-ebina k-ebina
Scopus Author ID
8415453300 8415453300
ORCID
0000-0002-2426-1024 0000-0002-2426-1024
CreatorName
Ebina, Kosuke
NRID
1000000432542 1000000432542
researchmap permalink
tanahiro-osk tanahiro-osk
Scopus Author ID
55628564591 55628564591
CreatorName
Tanaka, Hiroyuki
NRID
1000010527708 1000010527708
Scopus Author ID
8883255100 8883255100
CreatorName
Hirao, Makoto
NRID
1000040852108 1000040852108
researchmap permalink
tiwa tiwa
CreatorName
Iwahashi, Toru
NRID
1000050812294 1000050812294
researchmap permalink
takaakinoguchi takaakinoguchi
ORCID
0000-0001-9938-6780 0000-0001-9938-6780
CreatorName
Noguchi, Takaaki
CreatorName
Suzuki, Koji
CreatorName
Nishimoto, Shunsuke
NRID
1000050335361 1000050335361
ORCID
0000-0001-5628-5891 0000-0001-5628-5891
researchmap permalink
tmurase-osk tmurase-osk
Scopus Author ID
7202678305 7202678305
CreatorName
Murase, Tsuyoshi
NRID
1000060191558 1000060191558
CreatorName
Yoshikawa, Hideki
Subject
Dorsal root ganglion neurons
Neurite outgrowth
Oxygen ultra-fine bubbles
Peripheral nerve
Proliferation
Regeneration
Schwann cells
Sciatic nerve crush injury
Ultra-fine bubbles
Description
Matsuoka H., Ebina K., Tanaka H., et al. Administration of oxygen ultra-fine bubbles improves nerve dysfunction in a rat sciatic nerve crush injury model. International Journal of Molecular Sciences 19, 1395 (2018); https://doi.org/10.3390/ijms19051395.
Abstract
Ultra-fine bubbles (<200 nm in diameter) have several unique properties and have been tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUBs) on a sciatic nerve crush injury (SNC) model rats. Rats were intraperitoneally injected with 1.5 mL saline, OUBs diluted in saline, or nitrogen ultra-fine bubbles (NUBs) diluted in saline three times per week for 4 weeks in four groups: (1) control, (sham operation + saline); (2) SNC, (crush + saline); (3) SNC+OUB, (crush + OUB-saline); (4) SNC+NUB, (crush + NUB-saline). The effects of the OUBs on dorsal root ganglion (DRG) neurons and Schwann cells (SCs) were examined by serial dilution of OUB medium in vitro. Sciatic functional index, paw withdrawal thresholds, nerve conduction velocity, and myelinated axons were significantly decreased in the SNC group compared to the control group; these parameters were significantly improved in the SNC+OUB group, although NUB treatment did not affect these parameters. In vitro, OUBs significantly promoted neurite outgrowth in DRG neurons by activating AKT signaling and SC proliferation by activating ERK1/2 and JNK/c-JUN signaling. OUBs may improve nerve dysfunction in SNC rats by promoting neurite outgrowth in DRG neurons and SC proliferation.
Publisher
MDPI
Source Title
International Journal of Molecular Sciences
Volume (Issue)
19(5)
Page
1395
Date of Issued
2018-05-07
Language
English
Handle URL
https://hdl.handle.net/11094/93214
PISSN
16616596
eISSN
14220067
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.3390/ijms19051395
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article