Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice

Ebina, Kosuke; Shi, Kenrin; Hirao, Makoto; Hashimoto, Jun; Kawato, Yoshitaka; Kaneshiro, Shoichi; Morimoto, Tokimitsu; Koizumi, Kota; Yoshikawa, Hideki

doi:https://doi.org/10.1371/journal.pone.0065339

Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice

Ebina, Kosuke; Shi, Kenrin; Hirao, Makoto et al.

PLoS ONE, 2013, 8(6), e65339

Number of Access:831（2025-09-03 11:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93217

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Title	Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice
Creator	NRID 1000070612076 1000070612076 researchmap permalink k-ebina k-ebina Scopus Author ID 8415453300 8415453300 ORCID 0000-0002-2426-1024 0000-0002-2426-1024 CreatorName Ebina, Kosuke
	NRID 1000060575893 1000060575893 CreatorName Shi, Kenrin
	NRID 1000010527708 1000010527708 Scopus Author ID 8883255100 8883255100 CreatorName Hirao, Makoto
	NRID 1000040237938 1000040237938 CreatorName Hashimoto, Jun
	CreatorName Kawato, Yoshitaka
	CreatorName Kaneshiro, Shoichi
	CreatorName Morimoto, Tokimitsu
	CreatorName Koizumi, Kota
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, Hideki
Description	Ebina K., Shi K., Hirao M., et al. (2013) Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice. PLoS ONE 8(6): e65339. doi: 10.1371/journal.pone.0065339.
Abstract	Nanobubbles (<200 nm in diameter) have several unique properties such as long lifetime in liquid owing to its negatively charged surface, and its high gas solubility into the liquid owing to its high internal pressure. They are used in variety of fields including diagnostic aids and drug delivery, while there are no reports assessing their effects on the growth of lives. Nanobubbles of air or oxygen gas were generated using a nanobubble aerator (BUVITAS; Ligaric Company Limited, Osaka, Japan). Brassica campestris were cultured hydroponically for 4 weeks within air-nanobubble water or within normal water. Sweetfish (for 3 weeks) and rainbow trout (for 6 weeks) were kept either within air-nanobubble water or within normal water. Finally, 5 week-old male DBA1/J mice were bred with normal free-chaw and free-drinking either of oxygen-nanobubble water or of normal water for 12 weeks. Oxygen-nanobubble significantly increased the dissolved oxygen concentration of water as well as concentration/size of nanobubbles which were relatively stable for 70 days. Air-nanobubble water significantly promoted the height (19.1 vs. 16.7 cm; P<0.05), length of leaves (24.4 vs. 22.4 cm; P<0.01), and aerial fresh weight (27.3 vs. 20.3 g; P<0.01) of Brassica campestris compared to normal water. Total weight of sweetfish increased from 3.0 to 6.4 kg in normal water, whereas it increased from 3.0 to 10.2 kg in air-nanobubble water. In addition, total weight of rainbow trout increased from 50.0 to 129.5 kg in normal water, whereas it increased from 50.0 to 148.0 kg in air-nanobubble water. Free oral intake of oxygen-nanobubble water significantly promoted the weight (23.5 vs. 21.8 g; P<0.01) and the length (17.0 vs. 16.1 cm; P<0.001) of mice compared to that of normal water. We have demonstrated for the first time that oxygen and air-nanobubble water may be potentially effective tools for the growth of lives.
Publisher	Public Library of Scienceplos
Source Title	PLoS ONE
Volume (Issue)	8(6)
Page	e65339
Date of Issued	2013-06-05
Language	English
Handle URL	https://hdl.handle.net/11094/93217
eISSN	19326203
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1371/journal.pone.0065339
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	PLoS ONE. 2013, 8(6), p. e65339
DC.title	Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice
DC.creator	Ebina, Kosuke
	Shi, Kenrin
	Hirao, Makoto
	Hashimoto, Jun
	Kawato, Yoshitaka
	Kaneshiro, Shoichi
	Morimoto, Tokimitsu
	Koizumi, Kota
	Yoshikawa, Hideki
DC.publisher	Public Library of Scienceplos
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2013-06-05
DC.identifier	https://doi.org/10.1371/journal.pone.0065339
DC.description	Ebina K., Shi K., Hirao M., et al. (2013) Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice. PLoS ONE 8(6): e65339. doi: 10.1371/journal.pone.0065339.
DCTERMS.abstract	Nanobubbles (<200 nm in diameter) have several unique properties such as long lifetime in liquid owing to its negatively charged surface, and its high gas solubility into the liquid owing to its high internal pressure. They are used in variety of fields including diagnostic aids and drug delivery, while there are no reports assessing their effects on the growth of lives. Nanobubbles of air or oxygen gas were generated using a nanobubble aerator (BUVITAS; Ligaric Company Limited, Osaka, Japan). Brassica campestris were cultured hydroponically for 4 weeks within air-nanobubble water or within normal water. Sweetfish (for 3 weeks) and rainbow trout (for 6 weeks) were kept either within air-nanobubble water or within normal water. Finally, 5 week-old male DBA1/J mice were bred with normal free-chaw and free-drinking either of oxygen-nanobubble water or of normal water for 12 weeks. Oxygen-nanobubble significantly increased the dissolved oxygen concentration of water as well as concentration/size of nanobubbles which were relatively stable for 70 days. Air-nanobubble water significantly promoted the height (19.1 vs. 16.7 cm; P<0.05), length of leaves (24.4 vs. 22.4 cm; P<0.01), and aerial fresh weight (27.3 vs. 20.3 g; P<0.01) of Brassica campestris compared to normal water. Total weight of sweetfish increased from 3.0 to 6.4 kg in normal water, whereas it increased from 3.0 to 10.2 kg in air-nanobubble water. In addition, total weight of rainbow trout increased from 50.0 to 129.5 kg in normal water, whereas it increased from 50.0 to 148.0 kg in air-nanobubble water. Free oral intake of oxygen-nanobubble water significantly promoted the weight (23.5 vs. 21.8 g; P<0.01) and the length (17.0 vs. 16.1 cm; P<0.001) of mice compared to that of normal water. We have demonstrated for the first time that oxygen and air-nanobubble water may be potentially effective tools for the growth of lives.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93217
citation_title	Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice
citation_author	Ebina, Kosuke
	Shi, Kenrin
	Hirao, Makoto
	Hashimoto, Jun
	Kawato, Yoshitaka
	Kaneshiro, Shoichi
	Morimoto, Tokimitsu
	Koizumi, Kota
	Yoshikawa, Hideki
citation_publisher	Public Library of Scienceplos
citation_language	English
citation_date	2013-06-05
citation_journal_title	PLoS ONE
citation_volume	8
citation_issue	6
citation_firstpage	e65339
citation_doi	https://doi.org/10.1371/journal.pone.0065339
citation_public_url	https://hdl.handle.net/11094/93217

Oxygen and Air Nanobubble Water Solution Promote the Growth of Plants, Fishes, and Mice

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