Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice

Matsumoto, Ayumi; Matsui, Isao; Uchinomiya, Shohei; Katsuma, Yusuke; Yasuda, Seiichi; Okushima, Hiroki; Imai, Atsuhiro; Yamamoto, Takeshi; Ojida, Akio; Inoue, Kazunori; Isaka, Yoshitaka

doi:https://doi.org/10.1152/ajpendo.00147.2023

Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice

Matsumoto, Ayumi; Matsui, Isao; Uchinomiya, Shohei et al.

American Journal of Physiology - Endocrinology and Metabolism, 2023, 325(5), E552-E561

Number of Access:2,013（2025-11-04 11:21 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93228

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Title	Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice
Creator	NRID 1000040794053 1000040794053 CreatorName Matsumoto, Ayumi
	NRID 1000060456986 1000060456986 ORCID 0000-0001-8378-6130 0000-0001-8378-6130 researchmap permalink matsui-handai3857 matsui-handai3857 Scopus Author ID 24488796300 24488796300 CreatorName Matsui, Isao
	NRID 1000010770498 1000010770498 CreatorName Uchinomiya, Shohei
	CreatorName Katsuma, Yusuke
	CreatorName Yasuda, Seiichi
	NRID 1000070974632 1000070974632 CreatorName Okushima, Hiroki
	CreatorName Imai, Atsuhiro
	NRID 1000020756994 1000020756994 researchmap permalink tyamamoto-handai3857 tyamamoto-handai3857 Scopus Author ID 55628566248 55628566248 ORCID 0009-0007-3817-0557 0009-0007-3817-0557 CreatorName Yamamoto, Takeshi
	NRID 1000010343328 1000010343328 CreatorName Ojida, Akio
	NRID 1000010631301 1000010631301 researchmap permalink kznri kznri Scopus Author ID 55124465800 55124465800 ORCID 0000-0002-7149-6651 0000-0002-7149-6651 CreatorName Inoue, Kazunori
	NRID 1000000379166 1000000379166 researchmap permalink isaka-handai3857 isaka-handai3857 Scopus Author ID 16207611100 16207611100 ORCID 0000-0002-0820-7167 0000-0002-0820-7167 CreatorName Isaka, Yoshitaka
Subject	fatty acid b -oxidation
	in vivo imaging
	liver
	spatiotemporal resolution
Description	Matsumoto A., Matsui I., Uchinomiya S., et al. Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice. American Journal of Physiology - Endocrinology and Metabolism 325, E552 (2023); https://doi.org/10.1152/ajpendo.00147.2023.
Abstract	Mitochondrial fatty acid b-oxidation (FAO) plays a key role in energy homeostasis. Several FAO evaluation methods are currently available, but they are not necessarily suitable for capturing the dynamics of FAO in vivo at a cellular-level spatial resolution and seconds-level time resolution. FAOBlue is a coumarin-based probe that undergoes b-oxidation to produce a fluorescent substrate, 7-hydroxycoumarin-3-(N-(2-hydroxyethyl))-carboxamide (7-HC). After confirming that 7-HC could be specifically detected using multiphoton microscopy at excitation/emission wavelength ¼ 820/415–485 nm, wild-type C57BL/6 mice were randomly divided into control, pemafibrate, fasting (24 or 72 h), and etomoxir groups. These mice received a single intravenous injection of FAOBlue. FAO activities in the liver of these mice were visualized using multiphoton microscopy at 4.2 s/frame. These approaches could visualize the difference in FAO activities between periportal and pericentral hepatocytes in the control, pemafibrate, and fasting groups. FAO velocity, which was expressed by the maximum slope of the fluorescence intensity curve, was accelerated in the pemafibrate and 72-h fasting groups both in the periportal and the pericentral hepatocytes in comparison with the control group. Our approach revealed differences in the FAO activation mode by the two stimuli, i.e., pemafibrate and fasting, with pemafibrate accelerating the time of first detection of FAO-derived fluorescence. No increase in the fluorescence was observed in etomoxir-pretreated mice, confirming that FAOBlue specifically detected FAO in vivo. Thus, FAOBlue is useful for visualizing in vivo liver FAO dynamics at the single-cell-level spatial resolution and seconds-level time resolution.
Publisher	American Physiological Society
Source Title	American Journal of Physiology - Endocrinology and Metabolism
Volume (Issue)	325(5)
Page	E552-E561
Date of Issued	2023-11-01
Language	English
Handle URL	https://hdl.handle.net/11094/93228
PISSN	01931849
eISSN	15221555
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1152/ajpendo.00147.2023
Access Rights	open access
CopyRight	© 2023 the American Physiological Society.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	American Journal of Physiology - Endocrinology and Metabolism. 2023, 325(5), p. E552-E561
DC.title	Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice
DC.creator	Matsumoto, Ayumi
	Matsui, Isao
	Uchinomiya, Shohei
	Katsuma, Yusuke
	Yasuda, Seiichi
	Okushima, Hiroki
	Imai, Atsuhiro
	Yamamoto, Takeshi
	Ojida, Akio
	Inoue, Kazunori
	Isaka, Yoshitaka
DC.publisher	American Physiological Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-11-01
DC.identifier	https://doi.org/10.1152/ajpendo.00147.2023
DC.subject	fatty acid b -oxidation
	in vivo imaging
	liver
	spatiotemporal resolution
DC.description	Matsumoto A., Matsui I., Uchinomiya S., et al. Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice. American Journal of Physiology - Endocrinology and Metabolism 325, E552 (2023); https://doi.org/10.1152/ajpendo.00147.2023.
DCTERMS.abstract	Mitochondrial fatty acid b-oxidation (FAO) plays a key role in energy homeostasis. Several FAO evaluation methods are currently available, but they are not necessarily suitable for capturing the dynamics of FAO in vivo at a cellular-level spatial resolution and seconds-level time resolution. FAOBlue is a coumarin-based probe that undergoes b-oxidation to produce a fluorescent substrate, 7-hydroxycoumarin-3-(N-(2-hydroxyethyl))-carboxamide (7-HC). After confirming that 7-HC could be specifically detected using multiphoton microscopy at excitation/emission wavelength ¼ 820/415–485 nm, wild-type C57BL/6 mice were randomly divided into control, pemafibrate, fasting (24 or 72 h), and etomoxir groups. These mice received a single intravenous injection of FAOBlue. FAO activities in the liver of these mice were visualized using multiphoton microscopy at 4.2 s/frame. These approaches could visualize the difference in FAO activities between periportal and pericentral hepatocytes in the control, pemafibrate, and fasting groups. FAO velocity, which was expressed by the maximum slope of the fluorescence intensity curve, was accelerated in the pemafibrate and 72-h fasting groups both in the periportal and the pericentral hepatocytes in comparison with the control group. Our approach revealed differences in the FAO activation mode by the two stimuli, i.e., pemafibrate and fasting, with pemafibrate accelerating the time of first detection of FAO-derived fluorescence. No increase in the fluorescence was observed in etomoxir-pretreated mice, confirming that FAOBlue specifically detected FAO in vivo. Thus, FAOBlue is useful for visualizing in vivo liver FAO dynamics at the single-cell-level spatial resolution and seconds-level time resolution.
DC.rights	© 2023 the American Physiological Society.
DC.format	93228
citation_title	Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice
citation_author	Matsumoto, Ayumi
	Matsui, Isao
	Uchinomiya, Shohei
	Katsuma, Yusuke
	Yasuda, Seiichi
	Okushima, Hiroki
	Imai, Atsuhiro
	Yamamoto, Takeshi
	Ojida, Akio
	Inoue, Kazunori
	Isaka, Yoshitaka
citation_publisher	American Physiological Society
citation_language	English
citation_date	2023-11-01
citation_journal_title	American Journal of Physiology - Endocrinology and Metabolism
citation_volume	325
citation_issue	5
citation_firstpage	E552
citation_lastpage	E561
citation_doi	https://doi.org/10.1152/ajpendo.00147.2023
citation_public_url	https://hdl.handle.net/11094/93228

Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid b-oxidation at cellular-level resolution in mice

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