Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation

Noguchi, T.; Ebina, K.; Hirao, M.; Morimoto, T.; Koizumi, K.; Kitaguchi, K.; Matsuoka, H.; Iwahashi, T.; Yoshikawa, H.

doi:https://doi.org/10.1007/s00198-016-3830-1

Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation

Noguchi, T.; Ebina, K.; Hirao, M. et al.

Osteoporosis International, 2017, 28(3), 1063-1075

Number of Access:774（2025-09-03 11:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93256

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Title	Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation
Creator	CreatorName Noguchi, T.
	NRID 1000070612076 1000070612076 researchmap permalink k-ebina k-ebina Scopus Author ID 8415453300 8415453300 ORCID 0000-0002-2426-1024 0000-0002-2426-1024 CreatorName Ebina, K.
	NRID 1000010527708 1000010527708 CreatorName Hirao, M.
	CreatorName Morimoto, T.
	CreatorName Koizumi, K.
	CreatorName Kitaguchi, K.
	CreatorName Matsuoka, H.
	NRID 1000040852108 1000040852108 researchmap permalink tiwa tiwa CreatorName Iwahashi, T.
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, H.
Subject	c-Fos
	Glucocorticoid-induced osteoporosis (GIO)
	Nuclear factor of activated T-cells 1 (NFATc1)
	Osteoblasts
	Osteoclasts
	Oxygen ultra-fine bubbles (OUB)
Description	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00198-016-3830-1
Description	Noguchi T., Ebina K., Hirao M., et al. Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation. Osteoporosis International 28, 1063 (2017); https://doi.org/10.1007/s00198-016-3830-1.
Abstract	Summary: Oxygen ultra-fine bubbles (OUB) saline injection prevents bone loss of glucocorti\coid-induced osteoporosis in mice, and OUB inhibit osteoclastogenesis via RANK-TRAF6-c-Fos-NFATc1 signaling and RANK-p38 MAPK signaling in vitro. Introduction: Ultra-fine bubbles (<200 nm in diameter) have several unique properties, and they are tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUB) on glucocorticoid-induced osteoporosis (GIO) model mice. Methods: Prednisolone (PSL, 5 mg) was subcutaneously inserted in 6-month-old male C57BL/6J mice, and 200 μl of saline, OUB-diluted saline, or nitrogen ultra-fine bubbles (NUB)-diluted saline was intraperitoneally injected three times per week for 8 weeks the day after operations. Mice were divided into four groups; (1) control, sham-operation + saline; (2) GIO, PSL + saline; (3) GIO + OUB, PSL + OUB saline; (4) GIO + NUB, PSL + NUB saline. The effects of OUB on osteoblasts and osteoclasts were examined by serially diluted OUB medium in vitro. Results: Bone mass was significantly decreased in GIO [bone volume/total volume (%): control vs. GIO 12.6 vs. 7.9; p < 0.01] while significantly preserved in GIO + OUB (GIO vs. GIO + OUB 7.9 vs. 12.9; p < 0.05). In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the distal femur [mean osteoclasts number/bone surface (mm−1)] was significantly increased in GIO (control vs. GIO 6.8 vs. 11.6; p < 0.01) while suppressed in GIO + OUB (GIO vs. GIO + OUB 11.6 vs. 7.5; p < 0.01). NUB did not affect these parameters. In vitro experiments revealed that OUB significantly inhibited osteoclastogenesis by inhibiting RANK-TRAF6-c-Fos-NFATc1 signaling, RANK-p38 MAPK signaling, and TRAP/Cathepsin K/DC-STAMP mRNA expression in a concentration-dependent manner. OUB did not affect osteoblastogenesis in vitro. Conclusions: OUB prevent bone loss in GIO mice by inhibiting osteoclastogenesis.
Publisher	Springer Nature
Source Title	Osteoporosis International
Volume (Issue)	28(3)
Page	1063-1075
Date of Issued	2017-03-01
Language	English
Handle URL	https://hdl.handle.net/11094/93256
PISSN	0937941X
eISSN	14332965
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1007/s00198-016-3830-1
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Osteoporosis International. 2017, 28(3), p. 1063-1075
DC.title	Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation
DC.creator	Noguchi, T.
	Ebina, K.
	Hirao, M.
	Morimoto, T.
	Koizumi, K.
	Kitaguchi, K.
	Matsuoka, H.
	Iwahashi, T.
	Yoshikawa, H.
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-03-01
DC.identifier	https://doi.org/10.1007/s00198-016-3830-1
DC.subject	c-Fos
	Glucocorticoid-induced osteoporosis (GIO)
	Nuclear factor of activated T-cells 1 (NFATc1)
	Osteoblasts
	Osteoclasts
	Oxygen ultra-fine bubbles (OUB)
DC.description	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00198-016-3830-1
DC.description	Noguchi T., Ebina K., Hirao M., et al. Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation. Osteoporosis International 28, 1063 (2017); https://doi.org/10.1007/s00198-016-3830-1.
DCTERMS.abstract	Summary: Oxygen ultra-fine bubbles (OUB) saline injection prevents bone loss of glucocorti\coid-induced osteoporosis in mice, and OUB inhibit osteoclastogenesis via RANK-TRAF6-c-Fos-NFATc1 signaling and RANK-p38 MAPK signaling in vitro. Introduction: Ultra-fine bubbles (<200 nm in diameter) have several unique properties, and they are tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUB) on glucocorticoid-induced osteoporosis (GIO) model mice. Methods: Prednisolone (PSL, 5 mg) was subcutaneously inserted in 6-month-old male C57BL/6J mice, and 200 μl of saline, OUB-diluted saline, or nitrogen ultra-fine bubbles (NUB)-diluted saline was intraperitoneally injected three times per week for 8 weeks the day after operations. Mice were divided into four groups; (1) control, sham-operation + saline; (2) GIO, PSL + saline; (3) GIO + OUB, PSL + OUB saline; (4) GIO + NUB, PSL + NUB saline. The effects of OUB on osteoblasts and osteoclasts were examined by serially diluted OUB medium in vitro. Results: Bone mass was significantly decreased in GIO [bone volume/total volume (%): control vs. GIO 12.6 vs. 7.9; p < 0.01] while significantly preserved in GIO + OUB (GIO vs. GIO + OUB 7.9 vs. 12.9; p < 0.05). In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the distal femur [mean osteoclasts number/bone surface (mm−1)] was significantly increased in GIO (control vs. GIO 6.8 vs. 11.6; p < 0.01) while suppressed in GIO + OUB (GIO vs. GIO + OUB 11.6 vs. 7.5; p < 0.01). NUB did not affect these parameters. In vitro experiments revealed that OUB significantly inhibited osteoclastogenesis by inhibiting RANK-TRAF6-c-Fos-NFATc1 signaling, RANK-p38 MAPK signaling, and TRAP/Cathepsin K/DC-STAMP mRNA expression in a concentration-dependent manner. OUB did not affect osteoblastogenesis in vitro. Conclusions: OUB prevent bone loss in GIO mice by inhibiting osteoclastogenesis.
DC.format	93256
citation_title	Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation
citation_author	Noguchi, T.
	Ebina, K.
	Hirao, M.
	Morimoto, T.
	Koizumi, K.
	Kitaguchi, K.
	Matsuoka, H.
	Iwahashi, T.
	Yoshikawa, H.
citation_publisher	Springer Nature
citation_language	English
citation_date	2017-03-01
citation_journal_title	Osteoporosis International
citation_volume	28
citation_issue	3
citation_firstpage	1063
citation_lastpage	1075
citation_doi	https://doi.org/10.1007/s00198-016-3830-1
citation_public_url	https://hdl.handle.net/11094/93256

Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation

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