Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Matsuzaki, Takahisa; Kawano, Yuma; Horikiri, Momoka; Shimokawa, Yuko; Yamazaki, Takashi; Okuma, Nao; Koike, Hiroyuki; Kimura, Masaki; Kawamura, Ryuzo; Yoneyama, Yosuke; Furuichi, Yasuro; Hakuno, Fumihiko; Takahashi, Shin Ichiro; Nakabayashi, Seiichiro; Okamoto, Satoshi; Nakauchi, Hiromitsu; Taniguchi, Hideki; Takebe, Takanori; Yoshikawa, Hiroshi Y

doi:https://doi.org/10.1016/j.xpro.2023.102471

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Matsuzaki, Takahisa; Kawano, Yuma; Horikiri, Momoka et al.

STAR Protocols, 2023, 4(3), 102471

Number of Access:2,508（2025-07-31 23:24 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93286

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
STARProtoc_4_3_102471	pdf	None	4.12 MB	79	2023.12.11

Creative Commons : Attribution - NonCommercial - NoDerivatives

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells
Creator	NRID 1000050830527 1000050830527 ORCID 0000-0001-9466-7495 0000-0001-9466-7495 researchmap permalink 7000026401 7000026401 Scopus Author ID 55317252000 55317252000 CreatorName Matsuzaki, Takahisa
	CreatorName Kawano, Yuma
	CreatorName Horikiri, Momoka
	CreatorName Shimokawa, Yuko
	CreatorName Yamazaki, Takashi
	CreatorName Okuma, Nao
	CreatorName Koike, Hiroyuki
	CreatorName Kimura, Masaki
	NRID 1000050534591 1000050534591 CreatorName Kawamura, Ryuzo
	NRID 1000010748289 1000010748289 CreatorName Yoneyama, Yosuke
	NRID 1000040733035 1000040733035 CreatorName Furuichi, Yasuro
	NRID 1000030282700 1000030282700 CreatorName Hakuno, Fumihiko
	NRID 1000000197146 1000000197146 CreatorName Takahashi, Shin Ichiro
	NRID 1000070180346 1000070180346 CreatorName Nakabayashi, Seiichiro
	NRID 1000050509106 1000050509106 CreatorName Okamoto, Satoshi
	NRID 1000040175485 1000040175485 CreatorName Nakauchi, Hiromitsu
	NRID 1000070292555 1000070292555 CreatorName Taniguchi, Hideki
	NRID 1000020612625 1000020612625 researchmap permalink ttakebe ttakebe CreatorName Takebe, Takanori
	NRID 1000050551173 1000050551173 researchmap permalink read0108058 read0108058 Scopus Author ID 55708129100 55708129100 ORCID 0000-0003-0624-6039 0000-0003-0624-6039 CreatorName Yoshikawa, Hiroshi Y
Subject	Atomic Force Microscopy (AFM)
	Biophysics
	Biotechnology and Bioengineering
	Health Sciences
	Material Sciences
	Organoids
	Stem Cells
	Tissue Engineering
Description	Matsuzaki T., Kawano Y., Horikiri M., et al. Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells. STAR Protocols 4, 102471 (2023); https://doi.org/10.1016/j.xpro.2023.102471.
Abstract	Synthetic protocols providing mechanical patterns to culture substrate are essential to control the self-condensation of cells for organoid engineering. Here, we present a protocol for preparing hydrogels with mechanical patterns. We describe steps for hydrogel synthesis, mechanical evaluation of the substrate, and time-lapse imaging of cell self-organization. This protocol will facilitate the rational design of culture substrates with mechanical patterns for the engineering of various functional organoids. For complete details on the use and execution of this protocol, please refer to Takebe et al. (2015) and Matsuzaki et al. (2014, 2022).
Publisher	Cell Press
Source Title	STAR Protocols
Volume (Issue)	4(3)
Page	102471
Date of Issued	2023-09-15
Language	English
Handle URL	https://hdl.handle.net/11094/93286
eISSN	26661667
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.xpro.2023.102471
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	STAR Protocols. 2023, 4(3), p. 102471
DC.title	Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells
DC.creator	Matsuzaki, Takahisa
	Kawano, Yuma
	Horikiri, Momoka
	Shimokawa, Yuko
	Yamazaki, Takashi
	Okuma, Nao
	Koike, Hiroyuki
	Kimura, Masaki
	Kawamura, Ryuzo
	Yoneyama, Yosuke
	Furuichi, Yasuro
	Hakuno, Fumihiko
	Takahashi, Shin Ichiro
	Nakabayashi, Seiichiro
	Okamoto, Satoshi
	Nakauchi, Hiromitsu
	Taniguchi, Hideki
	Takebe, Takanori
	Yoshikawa, Hiroshi Y
DC.publisher	Cell Press
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-09-15
DC.identifier	https://doi.org/10.1016/j.xpro.2023.102471
DC.subject	Atomic Force Microscopy (AFM)
	Biophysics
	Biotechnology and Bioengineering
	Health Sciences
	Material Sciences
	Organoids
	Stem Cells
	Tissue Engineering
DC.description	Matsuzaki T., Kawano Y., Horikiri M., et al. Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells. STAR Protocols 4, 102471 (2023); https://doi.org/10.1016/j.xpro.2023.102471.
DCTERMS.abstract	Synthetic protocols providing mechanical patterns to culture substrate are essential to control the self-condensation of cells for organoid engineering. Here, we present a protocol for preparing hydrogels with mechanical patterns. We describe steps for hydrogel synthesis, mechanical evaluation of the substrate, and time-lapse imaging of cell self-organization. This protocol will facilitate the rational design of culture substrates with mechanical patterns for the engineering of various functional organoids. For complete details on the use and execution of this protocol, please refer to Takebe et al. (2015) and Matsuzaki et al. (2014, 2022).
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	93286
citation_title	Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells
citation_author	Matsuzaki, Takahisa
	Kawano, Yuma
	Horikiri, Momoka
	Shimokawa, Yuko
	Yamazaki, Takashi
	Okuma, Nao
	Koike, Hiroyuki
	Kimura, Masaki
	Kawamura, Ryuzo
	Yoneyama, Yosuke
	Furuichi, Yasuro
	Hakuno, Fumihiko
	Takahashi, Shin Ichiro
	Nakabayashi, Seiichiro
	Okamoto, Satoshi
	Nakauchi, Hiromitsu
	Taniguchi, Hideki
	Takebe, Takanori
	Yoshikawa, Hiroshi Y
citation_publisher	Cell Press
citation_language	English
citation_date	2023-09-15
citation_journal_title	STAR Protocols
citation_volume	4
citation_issue	3
citation_firstpage	102471
citation_doi	https://doi.org/10.1016/j.xpro.2023.102471
citation_public_url	https://hdl.handle.net/11094/93286

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Link to primary information

Item Information

Show details