Mechanical guidance of self-condensation patterns of differentiating progeny

Matsuzaki, Takahisa; Shimokawa, Yuko; Koike, Hiroyuki; Kimura, Masaki; Kawano, Yuma; Okuma, Nao; Kawamura, Ryuzo; Yoneyama, Yosuke; Furuichi, Yasuro; Hakuno, Fumihiko; Takahashi, Shin Ichiro; Nakabayashi, Seiichiro; Okamoto, Satoshi; Nakauchi, Hiromitsu; Taniguchi, Hideki; Takebe, Takanori; Yoshikawa, Hiroshi Y

doi:https://doi.org/10.1016/j.isci.2022.105109

Mechanical guidance of self-condensation patterns of differentiating progeny

Matsuzaki, Takahisa; Shimokawa, Yuko; Koike, Hiroyuki et al.

iScience, 2022, 25(10), 105109

Number of Access:2,386（2025-08-14 02:52 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93287

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
iScience_25_10_105109	pdf	None	3.54 MB	78	2023.12.11

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Mechanical guidance of self-condensation patterns of differentiating progeny
Creator	NRID 1000050830527 1000050830527 ORCID 0000-0001-9466-7495 0000-0001-9466-7495 researchmap permalink 7000026401 7000026401 Scopus Author ID 55317252000 55317252000 CreatorName Matsuzaki, Takahisa
	CreatorName Shimokawa, Yuko
	CreatorName Koike, Hiroyuki
	CreatorName Kimura, Masaki
	CreatorName Kawano, Yuma
	CreatorName Okuma, Nao
	NRID 1000050534591 1000050534591 CreatorName Kawamura, Ryuzo
	NRID 1000010748289 1000010748289 CreatorName Yoneyama, Yosuke
	NRID 1000040733035 1000040733035 CreatorName Furuichi, Yasuro
	NRID 1000030282700 1000030282700 CreatorName Hakuno, Fumihiko
	NRID 1000000197146 1000000197146 CreatorName Takahashi, Shin Ichiro
	NRID 1000070180346 1000070180346 CreatorName Nakabayashi, Seiichiro
	NRID 1000050509106 1000050509106 CreatorName Okamoto, Satoshi
	NRID 1000040175485 1000040175485 CreatorName Nakauchi, Hiromitsu
	NRID 1000070292555 1000070292555 CreatorName Taniguchi, Hideki
	NRID 1000020612625 1000020612625 researchmap permalink ttakebe ttakebe CreatorName Takebe, Takanori
	NRID 1000050551173 1000050551173 researchmap permalink read0108058 read0108058 Scopus Author ID 55708129100 55708129100 ORCID 0000-0003-0624-6039 0000-0003-0624-6039 CreatorName Yoshikawa, Hiroshi Y
Subject	Biomaterials
	Biomechanical engineering
	Mechanobiology
Description	Matsuzaki T., Shimokawa Y., Koike H., et al. Mechanical guidance of self-condensation patterns of differentiating progeny. iScience 25, 105109 (2022); https://doi.org/10.1016/j.isci.2022.105109.
Abstract	Spatially controlled self-organization represents a major challenge for organoid engineering. We have developed a mechanically patterned hydrogel for controlling self-condensation process to generate multi-cellular organoids. We first found that local stiffening with intrinsic mechanical gradient (IG > 0.008) induced single condensates of mesenchymal myoblasts, whereas the local softening led to stochastic aggregation. Besides, we revealed the cellular mechanism of two-step self-condensation: (1) cellular adhesion and migration at the mechanical boundary and (2) cell-cell contraction driven by intercellular actin-myosin networks. Finally, human pluripotent stem cell-derived hepatic progenitors with mesenchymal/endothelial cells (i.e., liver bud organoids) experienced collective migration toward locally stiffened regions generating condensates of the concave to spherical shapes. The underlying mechanism can be explained by force competition of cell-cell and cell-hydrogel biomechanical interactions between stiff and soft regions. These insights will facilitate the rational design of culture substrates inducing symmetry breaking in self-condensation of differentiating progeny toward future organoid engineering.
Publisher	Elsevier Inc.
Source Title	iScience
Volume (Issue)	25(10)
Page	105109
Date of Issued	2022-10-21
Language	English
Handle URL	https://hdl.handle.net/11094/93287
eISSN	25890042
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.isci.2022.105109
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	iScience. 2022, 25(10), p. 105109
DC.title	Mechanical guidance of self-condensation patterns of differentiating progeny
DC.creator	Matsuzaki, Takahisa
	Shimokawa, Yuko
	Koike, Hiroyuki
	Kimura, Masaki
	Kawano, Yuma
	Okuma, Nao
	Kawamura, Ryuzo
	Yoneyama, Yosuke
	Furuichi, Yasuro
	Hakuno, Fumihiko
	Takahashi, Shin Ichiro
	Nakabayashi, Seiichiro
	Okamoto, Satoshi
	Nakauchi, Hiromitsu
	Taniguchi, Hideki
	Takebe, Takanori
	Yoshikawa, Hiroshi Y
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-10-21
DC.identifier	https://doi.org/10.1016/j.isci.2022.105109
DC.subject	Biomaterials
	Biomechanical engineering
	Mechanobiology
DC.description	Matsuzaki T., Shimokawa Y., Koike H., et al. Mechanical guidance of self-condensation patterns of differentiating progeny. iScience 25, 105109 (2022); https://doi.org/10.1016/j.isci.2022.105109.
DCTERMS.abstract	Spatially controlled self-organization represents a major challenge for organoid engineering. We have developed a mechanically patterned hydrogel for controlling self-condensation process to generate multi-cellular organoids. We first found that local stiffening with intrinsic mechanical gradient (IG > 0.008) induced single condensates of mesenchymal myoblasts, whereas the local softening led to stochastic aggregation. Besides, we revealed the cellular mechanism of two-step self-condensation: (1) cellular adhesion and migration at the mechanical boundary and (2) cell-cell contraction driven by intercellular actin-myosin networks. Finally, human pluripotent stem cell-derived hepatic progenitors with mesenchymal/endothelial cells (i.e., liver bud organoids) experienced collective migration toward locally stiffened regions generating condensates of the concave to spherical shapes. The underlying mechanism can be explained by force competition of cell-cell and cell-hydrogel biomechanical interactions between stiff and soft regions. These insights will facilitate the rational design of culture substrates inducing symmetry breaking in self-condensation of differentiating progeny toward future organoid engineering.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93287
citation_title	Mechanical guidance of self-condensation patterns of differentiating progeny
citation_author	Matsuzaki, Takahisa
	Shimokawa, Yuko
	Koike, Hiroyuki
	Kimura, Masaki
	Kawano, Yuma
	Okuma, Nao
	Kawamura, Ryuzo
	Yoneyama, Yosuke
	Furuichi, Yasuro
	Hakuno, Fumihiko
	Takahashi, Shin Ichiro
	Nakabayashi, Seiichiro
	Okamoto, Satoshi
	Nakauchi, Hiromitsu
	Taniguchi, Hideki
	Takebe, Takanori
	Yoshikawa, Hiroshi Y
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2022-10-21
citation_journal_title	iScience
citation_volume	25
citation_issue	10
citation_firstpage	105109
citation_doi	https://doi.org/10.1016/j.isci.2022.105109
citation_public_url	https://hdl.handle.net/11094/93287

Mechanical guidance of self-condensation patterns of differentiating progeny

Link to primary information

Item Information

Show details