Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

Matsuzaki, Takahisa; Terutsuki, Daigo; Sato, Shoma; Ikarashi, Kohei; Sato, Kohei; Mitsuno, Hidefumi; Okumura, Ryu; Yoshimura, Yudai; Usami, Shigeyoshi; Mori, Yusuke; Fujii, Mai; Takemi, Shota; Nakabayashi, Seiichiro; Yoshikawa, Hiroshi Y; Kanzaki, Ryohei

doi:https://doi.org/10.1021/acs.jpclett.2c01673

Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

Matsuzaki, Takahisa; Terutsuki, Daigo; Sato, Shoma et al.

Journal of Physical Chemistry Letters, 2022, 13(40), 9494-9500

Number of Access:2,009（2025-07-31 23:24 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93288

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Title	Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature
Creator	NRID 1000050830527 1000050830527 ORCID 0000-0001-9466-7495 0000-0001-9466-7495 researchmap permalink 7000026401 7000026401 Scopus Author ID 55317252000 55317252000 CreatorName Matsuzaki, Takahisa
	NRID 1000040821921 1000040821921 CreatorName Terutsuki, Daigo
	CreatorName Sato, Shoma
	CreatorName Ikarashi, Kohei
	NRID 1000030756705 1000030756705 CreatorName Sato, Kohei
	NRID 1000060511855 1000060511855 CreatorName Mitsuno, Hidefumi
	NRID 1000000793449 1000000793449 researchmap permalink ryoharei301324 ryoharei301324 Scopus Author ID 55176912300 55176912300 CreatorName Okumura, Ryu
	CreatorName Yoshimura, Yudai
	NRID 1000030897947 1000030897947 researchmap permalink s_usami s_usami Scopus Author ID 56770778200 56770778200 ORCID 0000-0002-9710-1718 0000-0002-9710-1718 CreatorName Usami, Shigeyoshi
	NRID 1000090252618 1000090252618 ORCID 0000-0003-2952-7458 0000-0003-2952-7458 researchmap permalink mori_yusuke mori_yusuke Scopus Author ID 7404197134 7404197134 CreatorName Mori, Yusuke
	CreatorName Fujii, Mai
	NRID 1000070871440 1000070871440 CreatorName Takemi, Shota
	NRID 1000070180346 1000070180346 CreatorName Nakabayashi, Seiichiro
	NRID 1000050551173 1000050551173 researchmap permalink read0108058 read0108058 Scopus Author ID 55708129100 55708129100 ORCID 0000-0003-0624-6039 0000-0003-0624-6039 CreatorName Yoshikawa, Hiroshi Y
	NRID 1000040221907 1000040221907 CreatorName Kanzaki, Ryohei
Description	Matsuzaki T., Terutsuki D., Sato S., et al. Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature. Journal of Physical Chemistry Letters 2022 13(40), 9494-9500. DOI: 10.1021/acs.jpclett.2c01673. Copyright © 2022 American Chemical Society.
Abstract	Cell-coupled field-effect transistor (FET) biosensors have attracted considerable attention because of their high sensitivity to biomolecules. The use of insect cells (Sf21) as a core sensor element is advantageous due to their stable adhesion to sensors at room temperature. Although visualization of the insect cell-substrate interface leads to logical amplification of signals, the spatiotemporal processes at the interfaces have not yet been elucidated. We quantitatively monitored the adhesion dynamics of Sf21 using interference reflection microscopy (IRM). Specific adhesion signatures with ring-like patches along the cellular periphery were detected. A combination of zeta potential measurements and lectin staining identified specific glycoconjugates with low electrostatic potentials. The ring-like structures were disrupted after cholesterol depletion, suggesting a raft domain along the cell periphery. Our results indicate dynamic and asymmetric cell adhesion is due to low electrostatic repulsion with fluidic sugar rafts. We envision the logical design of cell-sensor interfaces with an electrical model that accounts for actual adhesion interfaces.
Publisher	American Chemical Society
Source Title	Journal of Physical Chemistry Letters
Volume (Issue)	13(40)
Page	9494-9500
Date of Issued	2022-10-13
Language	English
Handle URL	https://hdl.handle.net/11094/93288
eISSN	19487185
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1021/acs.jpclett.2c01673
Access Rights	open access
CopyRight	© 2022 American Chemical Society.
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Physical Chemistry Letters. 2022, 13(40), p. 9494-9500
DC.title	Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature
DC.creator	Matsuzaki, Takahisa
	Terutsuki, Daigo
	Sato, Shoma
	Ikarashi, Kohei
	Sato, Kohei
	Mitsuno, Hidefumi
	Okumura, Ryu
	Yoshimura, Yudai
	Usami, Shigeyoshi
	Mori, Yusuke
	Fujii, Mai
	Takemi, Shota
	Nakabayashi, Seiichiro
	Yoshikawa, Hiroshi Y
	Kanzaki, Ryohei
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-10-13
DC.identifier	https://doi.org/10.1021/acs.jpclett.2c01673
DC.description	Matsuzaki T., Terutsuki D., Sato S., et al. Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature. Journal of Physical Chemistry Letters 2022 13(40), 9494-9500. DOI: 10.1021/acs.jpclett.2c01673. Copyright © 2022 American Chemical Society.
DCTERMS.abstract	Cell-coupled field-effect transistor (FET) biosensors have attracted considerable attention because of their high sensitivity to biomolecules. The use of insect cells (Sf21) as a core sensor element is advantageous due to their stable adhesion to sensors at room temperature. Although visualization of the insect cell-substrate interface leads to logical amplification of signals, the spatiotemporal processes at the interfaces have not yet been elucidated. We quantitatively monitored the adhesion dynamics of Sf21 using interference reflection microscopy (IRM). Specific adhesion signatures with ring-like patches along the cellular periphery were detected. A combination of zeta potential measurements and lectin staining identified specific glycoconjugates with low electrostatic potentials. The ring-like structures were disrupted after cholesterol depletion, suggesting a raft domain along the cell periphery. Our results indicate dynamic and asymmetric cell adhesion is due to low electrostatic repulsion with fluidic sugar rafts. We envision the logical design of cell-sensor interfaces with an electrical model that accounts for actual adhesion interfaces.
DC.rights	© 2022 American Chemical Society.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93288
citation_title	Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature
citation_author	Matsuzaki, Takahisa
	Terutsuki, Daigo
	Sato, Shoma
	Ikarashi, Kohei
	Sato, Kohei
	Mitsuno, Hidefumi
	Okumura, Ryu
	Yoshimura, Yudai
	Usami, Shigeyoshi
	Mori, Yusuke
	Fujii, Mai
	Takemi, Shota
	Nakabayashi, Seiichiro
	Yoshikawa, Hiroshi Y
	Kanzaki, Ryohei
citation_publisher	American Chemical Society
citation_language	English
citation_date	2022-10-13
citation_journal_title	Journal of Physical Chemistry Letters
citation_volume	13
citation_issue	40
citation_firstpage	9494
citation_lastpage	9500
citation_doi	https://doi.org/10.1021/acs.jpclett.2c01673
citation_public_url	https://hdl.handle.net/11094/93288

Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

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