Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage

Chen, Xike; Yamashita, Akihiro; Morioka, Miho et al.

Tissue Engineering - Part A, 2019, 25(5-6), 437-445

Number of Access:819（2025-09-01 13:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93306

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Title
Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage
Creator
CreatorName
Chen, Xike
NRID
1000000636855 1000000636855
researchmap permalink
Aki_yama26 Aki_yama26
Scopus Author ID
56404044900 56404044900
CreatorName
Yamashita, Akihiro
CreatorName
Morioka, Miho
CreatorName
Senba, Toshika
NRID
1000090645764 1000090645764
CreatorName
Kamatani, Takashi
NRID
1000060506765 1000060506765
CreatorName
Watanabe, Akira
CreatorName
Kosai, Azuma
NRID
1000050303938 1000050303938
ORCID
0000-0002-0520-3654 0000-0002-0520-3654
researchmap permalink
cart cart
Scopus Author ID
7004069586 7004069586
CreatorName
Tsumaki, Noriyuki
Subject
cartilage
chondrocyte
FGF
iPS cells
perichondrium
Description
Chen X., Yamashita A., Morioka M., et al. Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage. Tissue Engineering - Part A 25, 437 (2019); https://doi.org/10.1089/ten.tea.2018.0133.
Abstract
New cell and tissue sources are needed for the regenerative treatment of articular cartilage damage. Human induced pluripotent stem cells (hiPSCs) are an abundant cell source due to their self-renewal capacity. Hyaline cartilage tissue particles derived from hiPSCs (hiPS-Carts), 1–3 mm in diameter, are one candidate source that can be used for transplantation. When transplanted to fill the defects of articular cartilage, hiPS-Carts form a repair tissue by integrating with each other and with adjacent host tissue. In this study, we analyzed the integration capacity using an in vitro model and found that hiPS-Carts spontaneously integrate with each other in vitro. hiPS-Carts consist of cartilage at the center and perichondrium-like membrane that wraps around the cartilage. The integration started at the perichondrium-like membrane at around 1 week. Then, the integration progressed to the cartilage within 4–8 weeks. RNA sequencing analysis identified a higher expression of FGF18 in the perichondrium-like membrane in hiPS-Carts compared with the central cartilage. The addition of FGF18 to the model accelerated the integration of hiPS-Carts, whereas the addition of a FGFR inhibitor inhibited it. These results suggest that FGF18 secreted from the perichondrium-like membrane plays a role in the integration of hiPS-Carts. Understanding the integration mechanism of hiPS-Carts is expected to contribute to the realization of regenerative treatment for patients with articular cartilage damage.
Publisher
Mary Ann Liebert Inc.
Source Title
Tissue Engineering - Part A
Volume (Issue)
25(5-6)
Page
437-445
Date of Issued
2019-04-17
Language
English
Handle URL
https://hdl.handle.net/11094/93306
PISSN
19373341
eISSN
1937335X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1089/ten.tea.2018.0133
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article