Ultrastiff Amyloid-Fibril Network of α-Synuclein Formed by Surface Seeding Reaction Confirmed by Multichannel Electrodeless Quartz-Crystal-Microbalance Biosensor

Zhou, Lianjie; Hajiri, Touko; Nakajima, Kichitaro et al.

ACS Sensors, 2023, 8(7), 2598-2608

Number of Access:1,038（2025-07-06 15:28 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93311

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Title
Ultrastiff Amyloid-Fibril Network of α-Synuclein Formed by Surface Seeding Reaction Confirmed by Multichannel Electrodeless Quartz-Crystal-Microbalance Biosensor
Creator
CreatorName
Zhou, Lianjie
CreatorName
Hajiri, Touko
NRID
1000020867337 1000020867337
ORCID
0000-0003-2644-741X 0000-0003-2644-741X
researchmap permalink
sonochem sonochem
Scopus Author ID
57145904700 57145904700
CreatorName
Nakajima, Kichitaro
NRID
1000020865597 1000020865597
researchmap permalink
cesarderkatter cesarderkatter
CreatorName
Aguirre, César
NRID
1000070774058 1000070774058
researchmap permalink
kensukeikenaka kensukeikenaka
Scopus Author ID
8588484300 8588484300
CreatorName
Ikenaka, Kensuke
NRID
1000090230044 1000090230044
ORCID
0000-0002-0874-7542 0000-0002-0874-7542
researchmap permalink
read0207787 read0207787
Scopus Author ID
7202006099 7202006099
CreatorName
Mochizuki, Hideki
NRID
1000090432187 1000090432187
researchmap permalink
read0105908 read0105908
Scopus Author ID
8731908100 8731908100
CreatorName
Yamaguchi, Keiichi
NRID
1000040153770 1000040153770
researchmap permalink
read0014440 read0014440
CreatorName
Goto, Yuji
NRID
1000090252626 1000090252626
researchmap permalink
read0042771 read0042771
Scopus Author ID
35563683500 35563683500
CreatorName
Ogi, Hirotsugu
Subject
Fibril Network
Multichannel QCM
Percolation
Stiffness
Wireless
α-Synuclein
Description
Zhou L., Hajiri T., Nakajima K., et al. Ultrastiff Amyloid-Fibril Network of α-Synuclein Formed by Surface Seeding Reaction Confirmed by Multichannel Electrodeless Quartz-Crystal-Microbalance Biosensor. ACS Sensors 8, 2598 (2023); https://doi.org/10.1021/acssensors.3c00331.
Abstract
We developed a multichannel wireless quartz-crystal-microbalance (QCM) biosensor for mechanically studying the on-surface aggregation reaction of α-synuclein (α-syn). We find a quite unusual change in the resonant frequency that eventually exceeds the baseline, which has never been observed during seeding aggregation reaction. By incorporating a growth-to-percolation theory for fibril elongation reaction, we have favorably reproduced this unusual response and found that it can be explained only with formation of an ultrastiff fibril network. We also find that the stiffness of the fibril network grown from artificially prepared twist-type seeds is significantly higher than that from rod-type seeds. Furthermore, the stiffnesses of fibril networks grown from seeds derived from brain tissues of Parkinson’s disease (PD) and multiple system atrophy (MSA) patients show a very similar trend to those of rod and twist seeds, respectively, indicating that fibrils from MSA patients are stiffer than those from PD.
Publisher
American Chemical Society
Source Title
ACS Sensors
Volume (Issue)
8(7)
Page
2598-2608
Date of Issued
2023-07-28
Language
English
Handle URL
https://hdl.handle.net/11094/93311
eISSN
23793694
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1021/acssensors.3c00331
Access Rights
open access
CopyRight
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sensors, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acssensors.3c00331.
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article