Development of HANABI, an ultrasonication-forced amyloid fibril inducer

Goto, Yuji; Nakajima, Kichitaro; Yamaguchi, Keiichi et al.

Neurochemistry International, 2022, 153, 105270

Number of Access:1,083（2025-08-16 09:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93312

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Title
Development of HANABI, an ultrasonication-forced amyloid fibril inducer
Creator
NRID
1000040153770 1000040153770
researchmap permalink
read0014440 read0014440
CreatorName
Goto, Yuji
NRID
1000020867337 1000020867337
ORCID
0000-0003-2644-741X 0000-0003-2644-741X
researchmap permalink
sonochem sonochem
Scopus Author ID
57145904700 57145904700
CreatorName
Nakajima, Kichitaro
NRID
1000090432187 1000090432187
researchmap permalink
read0105908 read0105908
Scopus Author ID
8731908100 8731908100
CreatorName
Yamaguchi, Keiichi
NRID
1000040746335 1000040746335
CreatorName
So, Masatomo
NRID
1000070774058 1000070774058
researchmap permalink
kensukeikenaka kensukeikenaka
Scopus Author ID
8588484300 8588484300
CreatorName
Ikenaka, Kensuke
NRID
1000090230044 1000090230044
ORCID
0000-0002-0874-7542 0000-0002-0874-7542
researchmap permalink
read0207787 read0207787
Scopus Author ID
7202006099 7202006099
CreatorName
Mochizuki, Hideki
NRID
1000090252626 1000090252626
researchmap permalink
read0042771 read0042771
Scopus Author ID
35563683500 35563683500
CreatorName
Ogi, Hirotsugu
Subject
Amyloid fibril
Solubility
Supersaturation
Ultrasonication
α-synuclein
β2-microglobulin
Description
Goto Y., Nakajima K., Yamaguchi K., et al. Development of HANABI, an ultrasonication-forced amyloid fibril inducer. Neurochemistry International 153, 105270 (2022); https://doi.org/10.1016/j.neuint.2021.105270.
Abstract
Amyloid fibrils involved in amyloidoses are crystal-like aggregates, which are formed by breaking supersaturation of denatured proteins. Ultrasonication is an efficient method of agitation for breaking supersaturation and thus inducing amyloid fibrils. By combining an ultrasonicator and a microplate reader, we developed the HANABI (HANdai Amyloid Burst Inducer) system that enables high-throughput analysis of amyloid fibril formation. Among high-throughput approaches of amyloid fibril assays, the HANABI system has advantages in accelerating and detecting spontaneous amyloid fibril formation. HANABI is also powerful for amplifying a tiny amount of preformed amyloid fibrils by seeding. Thus, HANABI will contribute to creating therapeutic strategies against amyloidoses by identifying their biomarkers.
Publisher
Elsevier Ltd
Source Title
Neurochemistry International
Volume (Issue)
153
Page
105270
Date of Issued
2022-02-01
Language
English
Handle URL
https://hdl.handle.net/11094/93312
PISSN
01970186
eISSN
18729754
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.neuint.2021.105270
Access Rights
open access
CopyRight
© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article