Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration

Terada, Eisaku; Bamba, Yohei; Takagaki, Masatoshi; Kawabata, Shuhei; Tachi, Tetsuro; Nakamura, Hajime; Nishida, Takeo; Kishima, Haruhiko

doi:https://doi.org/10.1093/stcltm/szad066

Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration

Terada, Eisaku; Bamba, Yohei; Takagaki, Masatoshi et al.

Stem Cells Translational Medicine, 2023, 12(12), 825-837

Number of Access:1,024（2025-08-16 09:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93317

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Title	Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration
Creator	NRID 1000040869541 1000040869541 researchmap permalink teradaeisaku teradaeisaku CreatorName Terada, Eisaku
	NRID 1000020577465 1000020577465 CreatorName Bamba, Yohei
	NRID 1000070724433 1000070724433 researchmap permalink osakansmt osakansmt Scopus Author ID 46261664000 46261664000 CreatorName Takagaki, Masatoshi
	NRID 1000020764062 1000020764062 researchmap permalink shuheiK shuheiK CreatorName Kawabata, Shuhei
	CreatorName Tachi, Tetsuro
	NRID 1000080533794 1000080533794 researchmap permalink hnns-osaka hnns-osaka Scopus Author ID 55624478238 55624478238 CreatorName Nakamura, Hajime
	NRID 1000030533806 1000030533806 CreatorName Nishida, Takeo
	NRID 1000010332743 1000010332743 researchmap permalink hkishima hkishima Scopus Author ID 6603783358 6603783358 ORCID 0000-0002-9041-2337 0000-0002-9041-2337 CreatorName Kishima, Haruhiko
Description	Terada Eisaku, Bamba Yohei, Takagaki Masatoshi, et al. Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration. Stem Cells Translational Medicine 9, 519 (2023); https://doi.org/10.1093/stcltm/szad066.
Abstract	Cerebral organoids (COs) are derived from human-induced pluripotent stem cells in vitro and mimic the features of the human fetal brain. The development of COs is largely dependent on “self-organization” mechanisms, in which differentiating cells committed to cortical cells autonomously organize into the cerebral cortex-like tissue. However, extrinsic manipulation of their morphology, including size and thickness, remains challenging. In this study, we discovered that silicate microfiber scaffolds could support the formation of cortical neuronal layers and successfully generated cortical neuronal layers, which are 9 times thicker than conventional COs, in 70 days. These cortical neurons in the silicate microfiber layer were differentiated in a fetal brain-like lamination pattern. While these cellular characteristics such as cortical neurons and neural stem/progenitor cells were like those of conventional COs, the cortical neuronal layers were greatly thickened in sheet-like configuration. Moreover, the cortical neurons in the scaffolds showed spontaneous electrical activity. We concluded that silicate microfiber scaffolds support the formation of the cortical neuronal layers of COs without disturbing self-organization-driven corticogenesis. The extrinsic manipulation of the formation of the cortical neuronal layers of COs may be useful for the research of developmental mechanisms or pathogenesis of the human cerebral cortex, particularly for the development of regenerative therapy and bioengineering.
Publisher	Oxford University Press (OUP)
Source Title	Stem Cells Translational Medicine
Volume (Issue)	12(12)
Page	825-837
Date of Issued	2023-10-16
Language	English
Handle URL	https://hdl.handle.net/11094/93317
PISSN	21576564
eISSN	21576580
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1093/stcltm/szad066
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Stem Cells Translational Medicine. 2023, 12(12), p. 825-837
DC.title	Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration
DC.creator	Terada, Eisaku
	Bamba, Yohei
	Takagaki, Masatoshi
	Kawabata, Shuhei
	Tachi, Tetsuro
	Nakamura, Hajime
	Nishida, Takeo
	Kishima, Haruhiko
DC.publisher	Oxford University Press (OUP)
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-10-16
DC.identifier	https://doi.org/10.1093/stcltm/szad066
DC.description	Terada Eisaku, Bamba Yohei, Takagaki Masatoshi, et al. Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration. Stem Cells Translational Medicine 9, 519 (2023); https://doi.org/10.1093/stcltm/szad066.
DCTERMS.abstract	Cerebral organoids (COs) are derived from human-induced pluripotent stem cells in vitro and mimic the features of the human fetal brain. The development of COs is largely dependent on “self-organization” mechanisms, in which differentiating cells committed to cortical cells autonomously organize into the cerebral cortex-like tissue. However, extrinsic manipulation of their morphology, including size and thickness, remains challenging. In this study, we discovered that silicate microfiber scaffolds could support the formation of cortical neuronal layers and successfully generated cortical neuronal layers, which are 9 times thicker than conventional COs, in 70 days. These cortical neurons in the silicate microfiber layer were differentiated in a fetal brain-like lamination pattern. While these cellular characteristics such as cortical neurons and neural stem/progenitor cells were like those of conventional COs, the cortical neuronal layers were greatly thickened in sheet-like configuration. Moreover, the cortical neurons in the scaffolds showed spontaneous electrical activity. We concluded that silicate microfiber scaffolds support the formation of the cortical neuronal layers of COs without disturbing self-organization-driven corticogenesis. The extrinsic manipulation of the formation of the cortical neuronal layers of COs may be useful for the research of developmental mechanisms or pathogenesis of the human cerebral cortex, particularly for the development of regenerative therapy and bioengineering.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93317
citation_title	Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration
citation_author	Terada, Eisaku
	Bamba, Yohei
	Takagaki, Masatoshi
	Kawabata, Shuhei
	Tachi, Tetsuro
	Nakamura, Hajime
	Nishida, Takeo
	Kishima, Haruhiko
citation_publisher	Oxford University Press (OUP)
citation_language	English
citation_date	2023-10-16
citation_journal_title	Stem Cells Translational Medicine
citation_volume	12
citation_issue	12
citation_firstpage	825
citation_lastpage	837
citation_doi	https://doi.org/10.1093/stcltm/szad066
citation_public_url	https://hdl.handle.net/11094/93317

Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration

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