A rapid and simple electrochemical detection of the free drug concentration in human serum using boron-doped diamond electrodes

Moriyama, Hideto; Ogata, Genki; Nashimoto, Haruma; Sawamura, Seishiro; Furukawa, Yoshiaki; Hibino, Hiroshi; Kusuhara, Hiroyuki; Einaga, Yasuaki

doi:https://doi.org/10.1039/d2an01037b

A rapid and simple electrochemical detection of the free drug concentration in human serum using boron-doped diamond electrodes

Moriyama, Hideto; Ogata, Genki; Nashimoto, Haruma et al.

Analyst, 2022, 147(20), 4442-4449

Number of Access:691（2025-08-15 06:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93342

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Title	A rapid and simple electrochemical detection of the free drug concentration in human serum using boron-doped diamond electrodes
Creator	CreatorName Moriyama, Hideto
	NRID 1000080452829 1000080452829 CreatorName Ogata, Genki
	CreatorName Nashimoto, Haruma
	NRID 1000010781974 1000010781974 Scopus Author ID 57190878168 57190878168 CreatorName Sawamura, Seishiro
	NRID 1000040415287 1000040415287 CreatorName Furukawa, Yoshiaki
	NRID 1000070314317 1000070314317 researchmap permalink hiroshi_hibino hiroshi_hibino Scopus Author ID 7007020846 7007020846 CreatorName Hibino, Hiroshi
	NRID 1000000302612 1000000302612 CreatorName Kusuhara, Hiroyuki
	NRID 1000000322066 1000000322066 CreatorName Einaga, Yasuaki
Description	Reproduced from Analyst., 2022, 147, 4442-4449 with permission from the Royal Society of Chemistry.
Description	https://doi.org/10.1039/d2an01037b
Abstract	Monitoring drug concentration in blood and reflecting this in the dosage are crucial for safe and effective drug treatment. Most drug assays are based on total concentrations of bound and unbound proteins in the serum, although only the unbound concentration causes beneficial and adverse events. Monitoring the unbound concentration alone is expected to provide a means for further optimisation of drug treatment. However, unbound concentration monitoring has not been routinely used for drug treatment due to the long analysis time and the high cost of conventional methods. Here, we have developed a rapid electrochemical method to determine the unbound concentration in ultrafiltered human serum using boron-doped diamond (BDD) electrodes. When the anticancer drug doxorubicin was used as the test drug, the catalytic doxorubicin-mediated reduction of dissolved oxygen provided a sensitive electrochemical signal, with a detection limit of 0.14 nM. In contrast, the sensitivity of glassy carbon (GC) was inferior under the same conditions due to interference from the dissolved oxygen reduction current. The signal background ratio (S/B) of BDD and GC was 11.5 (10 nM doxorubicin) and 1.1 (50 nM), respectively. The results show that a fast measurement time within ten seconds is possible in the clinical concentration range. Additionally, in the ultrafiltered human serum, the obtained values of unbound doxorubicin concentration showed good agreement with those quantified by conventional liquid chromatography-mass spectrometry. This approach has the potential for application in clinical settings where rapid and simple analysis methods would be beneficial.
Publisher	Royal Society of Chemistry
Source Title	Analyst
Volume (Issue)	147(20)
Page	4442-4449
Date of Issued	2022-09-13
Language	English
Handle URL	https://hdl.handle.net/11094/93342
PISSN	00032654
eISSN	13645528
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1039/d2an01037b
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analyst. 2022, 147(20), p. 4442-4449
DC.title	A rapid and simple electrochemical detection of the free drug concentration in human serum using boron-doped diamond electrodes
DC.creator	Moriyama, Hideto
	Ogata, Genki
	Nashimoto, Haruma
	Sawamura, Seishiro
	Furukawa, Yoshiaki
	Hibino, Hiroshi
	Kusuhara, Hiroyuki
	Einaga, Yasuaki
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-09-13
DC.identifier	https://doi.org/10.1039/d2an01037b
DC.description	Reproduced from Analyst., 2022, 147, 4442-4449 with permission from the Royal Society of Chemistry.
DC.description	https://doi.org/10.1039/d2an01037b
DCTERMS.abstract	Monitoring drug concentration in blood and reflecting this in the dosage are crucial for safe and effective drug treatment. Most drug assays are based on total concentrations of bound and unbound proteins in the serum, although only the unbound concentration causes beneficial and adverse events. Monitoring the unbound concentration alone is expected to provide a means for further optimisation of drug treatment. However, unbound concentration monitoring has not been routinely used for drug treatment due to the long analysis time and the high cost of conventional methods. Here, we have developed a rapid electrochemical method to determine the unbound concentration in ultrafiltered human serum using boron-doped diamond (BDD) electrodes. When the anticancer drug doxorubicin was used as the test drug, the catalytic doxorubicin-mediated reduction of dissolved oxygen provided a sensitive electrochemical signal, with a detection limit of 0.14 nM. In contrast, the sensitivity of glassy carbon (GC) was inferior under the same conditions due to interference from the dissolved oxygen reduction current. The signal background ratio (S/B) of BDD and GC was 11.5 (10 nM doxorubicin) and 1.1 (50 nM), respectively. The results show that a fast measurement time within ten seconds is possible in the clinical concentration range. Additionally, in the ultrafiltered human serum, the obtained values of unbound doxorubicin concentration showed good agreement with those quantified by conventional liquid chromatography-mass spectrometry. This approach has the potential for application in clinical settings where rapid and simple analysis methods would be beneficial.
DC.format	93342
citation_title	A rapid and simple electrochemical detection of the free drug concentration in human serum using boron-doped diamond electrodes
citation_author	Moriyama, Hideto
	Ogata, Genki
	Nashimoto, Haruma
	Sawamura, Seishiro
	Furukawa, Yoshiaki
	Hibino, Hiroshi
	Kusuhara, Hiroyuki
	Einaga, Yasuaki
citation_publisher	Royal Society of Chemistry
citation_language	English
citation_date	2022-09-13
citation_journal_title	Analyst
citation_volume	147
citation_issue	20
citation_firstpage	4442
citation_lastpage	4449
citation_doi	https://doi.org/10.1039/d2an01037b
citation_public_url	https://hdl.handle.net/11094/93342

A rapid and simple electrochemical detection of the free drug concentration in human serum using boron-doped diamond electrodes

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