In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes

Hanawa, Ai; Ogata, Genki; Sawamura, Seishiro; Asai, Kai; Kanzaki, Sho; Hibino, Hiroshi; Einaga, Yasuaki

doi:https://doi.org/10.1021/acs.analchem.0c01707

In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes

Hanawa, Ai; Ogata, Genki; Sawamura, Seishiro et al.

Analytical Chemistry, 2020, 92(20), 13742-13749

Number of Access:656（2025-08-13 21:17 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93343

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Title	In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes
Creator	CreatorName Hanawa, Ai
	NRID 1000080452829 1000080452829 CreatorName Ogata, Genki
	NRID 1000010781974 1000010781974 Scopus Author ID 57190878168 57190878168 CreatorName Sawamura, Seishiro
	CreatorName Asai, Kai
	NRID 1000050286556 1000050286556 CreatorName Kanzaki, Sho
	NRID 1000070314317 1000070314317 researchmap permalink hiroshi_hibino hiroshi_hibino Scopus Author ID 7007020846 7007020846 CreatorName Hibino, Hiroshi
	NRID 1000000322066 1000000322066 CreatorName Einaga, Yasuaki
Description	Hanawa A., Ogata G., Sawamura S., et al. In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes. Analytical Chemistry 92(20), 13742 (2020); https://doi.org/10.1021/acs.analchem.0c01707.
Abstract	Methylcobalamin, which is used for the clinical treatment of patients with neuropathy, can have an impact on the sensorineural components associated with the cochlea, and it is possible that the auditory threshold in a certain population of patients with deafness may be recovered. Nonetheless, it remains uncertain whether the action site of methylcobalamin is localized inside or outside the cochlea and which cellular or tissue element is targeted by the drug. In the present work, we developed a method to realize in vivo real-time simultaneous examination of the drug kinetics in two separate locations using boron-doped diamond microelectrodes. First, the analytical performance of methylcobalamin was studied and the measurement protocol was optimized in vitro. Then, the optimized protocol was applied to carry out real-time measurements inside the cochlea and the leg muscle in live guinea pigs while systemically administering methylcobalamin. The results showed that the methylcobalamin concentration in the cochlea was below the limit of detection for the microelectrodes or the drug did not reach the cochlea, whereas the compound clearly reached the leg muscle.
Publisher	American Chemical Society
Source Title	Analytical Chemistry
Volume (Issue)	92(20)
Page	13742-13749
Date of Issued	2020-10-20
Language	English
Handle URL	https://hdl.handle.net/11094/93343
PISSN	00032700
eISSN	15206882
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1021/acs.analchem.0c01707
Access Rights	open access
CopyRight	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.0c01707.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analytical Chemistry. 2020, 92(20), p. 13742-13749
DC.title	In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes
DC.creator	Hanawa, Ai
	Ogata, Genki
	Sawamura, Seishiro
	Asai, Kai
	Kanzaki, Sho
	Hibino, Hiroshi
	Einaga, Yasuaki
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-10-20
DC.identifier	https://doi.org/10.1021/acs.analchem.0c01707
DC.description	Hanawa A., Ogata G., Sawamura S., et al. In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes. Analytical Chemistry 92(20), 13742 (2020); https://doi.org/10.1021/acs.analchem.0c01707.
DCTERMS.abstract	Methylcobalamin, which is used for the clinical treatment of patients with neuropathy, can have an impact on the sensorineural components associated with the cochlea, and it is possible that the auditory threshold in a certain population of patients with deafness may be recovered. Nonetheless, it remains uncertain whether the action site of methylcobalamin is localized inside or outside the cochlea and which cellular or tissue element is targeted by the drug. In the present work, we developed a method to realize in vivo real-time simultaneous examination of the drug kinetics in two separate locations using boron-doped diamond microelectrodes. First, the analytical performance of methylcobalamin was studied and the measurement protocol was optimized in vitro. Then, the optimized protocol was applied to carry out real-time measurements inside the cochlea and the leg muscle in live guinea pigs while systemically administering methylcobalamin. The results showed that the methylcobalamin concentration in the cochlea was below the limit of detection for the microelectrodes or the drug did not reach the cochlea, whereas the compound clearly reached the leg muscle.
DC.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.0c01707.
DC.format	93343
citation_title	In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes
citation_author	Hanawa, Ai
	Ogata, Genki
	Sawamura, Seishiro
	Asai, Kai
	Kanzaki, Sho
	Hibino, Hiroshi
	Einaga, Yasuaki
citation_publisher	American Chemical Society
citation_language	English
citation_date	2020-10-20
citation_journal_title	Analytical Chemistry
citation_volume	92
citation_issue	20
citation_firstpage	13742
citation_lastpage	13749
citation_doi	https://doi.org/10.1021/acs.analchem.0c01707
citation_public_url	https://hdl.handle.net/11094/93343

In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes

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