A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain

Ino, Daisuke; Tanaka, Yudai; Hibino, Hiroshi; Nishiyama, Masaaki

doi:https://doi.org/10.1038/s41592-022-01597-x

A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain

Ino, Daisuke; Tanaka, Yudai; Hibino, Hiroshi et al.

Nature Methods, 2022, 19(10), 1286-1294

Number of Access:551（2025-07-27 01:59 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93409

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Title	A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
Creator	NRID 1000040721981 1000040721981 ORCID 0000-0002-8112-0746 0000-0002-8112-0746 researchmap permalink daisukeino daisukeino CreatorName Ino, Daisuke
	CreatorName Tanaka, Yudai
	NRID 1000070314317 1000070314317 researchmap permalink hiroshi_hibino hiroshi_hibino Scopus Author ID 7007020846 7007020846 CreatorName Hibino, Hiroshi
	NRID 1000050423562 1000050423562 CreatorName Nishiyama, Masaaki
Description	Ino D., Tanaka Y., Hibino H., et al. A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Nature Methods 19, 1286 (2022); https://doi.org/10.1038/s41592-022-01597-x.
Abstract	Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIAOT, a G-protein-coupled receptor-based green fluorescent OT sensor that has a large dynamic range, suitable affinity, ligand specificity for OT orthologs, minimal effects on downstream signaling and long-term fluorescence stability. By combining viral gene delivery and fiber photometry-mediated fluorescence measurements, we demonstrate the utility of MTRIAOT for real-time detection of brain OT dynamics in living mice. MTRIAOT-mediated measurements indicate variability of OT dynamics depending on the behavioral context and physical condition of an animal. MTRIAOT will likely enable the analysis of OT dynamics in a variety of physiological and pathological processes.
Publisher	Nature Research
Source Title	Nature Methods
Volume (Issue)	19(10)
Page	1286-1294
Date of Issued	2022-10-01
Language	English
Handle URL	https://hdl.handle.net/11094/93409
PISSN	15487091
eISSN	15487105
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s41592-022-01597-x
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Nature Methods. 2022, 19(10), p. 1286-1294
DC.title	A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
DC.creator	Ino, Daisuke
	Tanaka, Yudai
	Hibino, Hiroshi
	Nishiyama, Masaaki
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-10-01
DC.identifier	https://doi.org/10.1038/s41592-022-01597-x
DC.description	Ino D., Tanaka Y., Hibino H., et al. A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Nature Methods 19, 1286 (2022); https://doi.org/10.1038/s41592-022-01597-x.
DCTERMS.abstract	Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIAOT, a G-protein-coupled receptor-based green fluorescent OT sensor that has a large dynamic range, suitable affinity, ligand specificity for OT orthologs, minimal effects on downstream signaling and long-term fluorescence stability. By combining viral gene delivery and fiber photometry-mediated fluorescence measurements, we demonstrate the utility of MTRIAOT for real-time detection of brain OT dynamics in living mice. MTRIAOT-mediated measurements indicate variability of OT dynamics depending on the behavioral context and physical condition of an animal. MTRIAOT will likely enable the analysis of OT dynamics in a variety of physiological and pathological processes.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93409
citation_title	A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
citation_author	Ino, Daisuke
	Tanaka, Yudai
	Hibino, Hiroshi
	Nishiyama, Masaaki
citation_publisher	Nature Research
citation_language	English
citation_date	2022-10-01
citation_journal_title	Nature Methods
citation_volume	19
citation_issue	10
citation_firstpage	1286
citation_lastpage	1294
citation_doi	https://doi.org/10.1038/s41592-022-01597-x
citation_public_url	https://hdl.handle.net/11094/93409

A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain

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