Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae

Nin, Fumiaki; Choi, Samuel; Ota, Takeru et al.

Optical Review, 2021, 28(2), 239-254

Number of Access:650（2025-08-15 09:42 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93413

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Title
Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae
Creator
NRID
1000080644905 1000080644905
CreatorName
Nin, Fumiaki
NRID
1000060568418 1000060568418
CreatorName
Choi, Samuel
NRID
1000030790571 1000030790571
ORCID
0000-0003-0011-8925 0000-0003-0011-8925
researchmap permalink
otatakeru otatakeru
Scopus Author ID
26642091900 26642091900
CreatorName
Ota, Takeru
CreatorName
Qi, Zhang
NRID
1000070314317 1000070314317
researchmap permalink
hiroshi_hibino hiroshi_hibino
Scopus Author ID
7007020846 7007020846
CreatorName
Hibino, Hiroshi
Subject
Cochlear partition
In vivo vibrometry
Optical coherence tomography
Organ of Corti
Outer hair cells
Supercontinuum
Description
The version of record of this article, first published in Optical Review, is available online at Publisher’s website: https://doi.org/10.1007/s10043-021-00654-8.
Abstract
Sound evokes sub-nanoscale vibration within the sensory epithelium. The epithelium contains not only immotile cells but also contractile outer hair cells (OHCs) that actively shrink and elongate synchronously with the sound. However, the in vivo motion of OHCs has remained undetermined. The aim of this work is to perform high-resolution and -accuracy vibrometry in live guinea pigs with an SC-introduced spectral-domain optical coherence tomography system (SD-OCT). In this study, to reveal the effective contribution of SC source in the recording of the low reflective materials with the short total acquisition time, we compare the performances of the SC-introduced SD-OCT (SCSD-OCT) to that of the conventional SD-OCT. As inanimate comparison objects, we record a mirror, a piezo actuator, and glass windows. For the measurements in biological materials, we use in/ex vivo guinea pig cochleae. Our study achieved the optimization of a SD-OCT system for high-resolution in vivo vibrometry in the cochlear sensory epithelium, termed the organ of Corti, in mammalian cochlea. By introducing a supercontinuum (SC) light source and reducing the total acquisition time, we improve the axial resolution and overcome the difficulty in recording the low reflective material in the presence of biological noise. The high power of the SC source enables the system to achieve a spatial resolution of 1.72 ± 0.00 μm on a mirror and reducing the total acquisition time contributes to the high spatial accuracy of sub-nanoscale vibrometry. Our findings reveal the vibrations at the apical/basal region of OHCs and the extracellular matrix, basilar membrane.
Publisher
Springer Science and Business Media LLC
Source Title
Optical Review
Volume (Issue)
28(2)
Page
239-254
Date of Issued
2021-04-01
Language
English
Handle URL
https://hdl.handle.net/11094/93413
PISSN
13406000
eISSN
13499432
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s10043-021-00654-8
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article