PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells

Matsugaki, Aira; Matsuzaka, Tadaaki; Mori, Toko; Saito, Mitsuka; Funaoku, Kazuma; Yamano, Riku; Gokcekaya, Ozkan; Ozasa, Ryosuke; Nakano, Takayoshi

doi:https://doi.org/10.36922/ijb.1425

PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells

Matsugaki, Aira; Matsuzaka, Tadaaki; Mori, Toko et al.

International Journal of Bioprinting, 2024, 10(1), 1425

Number of Access:656（2025-07-27 21:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93524

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Title	PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells
Creator	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	CreatorName Matsuzaka, Tadaaki
	CreatorName Mori, Toko
	CreatorName Saito, Mitsuka
	CreatorName Funaoku, Kazuma
	CreatorName Yamano, Riku
	CreatorName Gokcekaya, Ozkan
	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Additive manufacturing
	Laser powder bed fusion
	Mesenchymal stem
	Osteogenic differentiation
	Bone microstructure
Description	Matsugaki Aira, Matsuzaka Tadaaki, Mori Toko, et al. PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells. International Journal of Bioprinting 10, 1425 (2024); https://doi.org/10.36922/ijb.1425.
Abstract	Stem cell differentiation has important implications for biomedical device design and tissue engineering. Recently, inherent material properties, including surface chemistry, stiffness, and topography, have been found to influence stem cell fate. Among these, surface topography is a key regulator of stem cells in contact with materials. The most important aspect of ideal bone tissue engineering is to control the organization of the bone extracellular matrix with fully differentiated osteoblasts. Here, we found that laser powder bed fusion (PBF-LB)-fabricated grooved surface inspired by the microstructure of bone, which induced human mesenchymal stem cell (hMSC) differentiation into the osteogenic lineage without any differentiation supplements. The periodic grooved structure was fabricated by PBF-LB which induced cell elongation facilitated by cytoskeletal tension along the grooves. This resulted in the upregulation of osteogenesis via Runx2 expression. The aligned hMSCs successfully differentiated into osteoblasts and further organized the bone mimetic-oriented extracellular matrix microstructure. Our results indicate that metal additive manufacturing technology has a great advantage in controlling stem cell fate into the osteogenic lineage, and in the construction of bone-mimetic microstructural organization. Our findings on material-induced stem cell differentiation under standard cell culture conditions open new avenues for the development of medical devices that realize the desired tissue regeneration mediated by regulated stem cell functions.
Publisher	AccScience Publishing
Source Title	International Journal of Bioprinting
Volume (Issue)	10(1)
Page	1425
Date of Issued	2024-01-09
Language	English
Handle URL	https://hdl.handle.net/11094/93524
PISSN	24247723
eISSN	24248002
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.36922/ijb.1425
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	International Journal of Bioprinting. 2024, 10(1), p. 1425
DC.title	PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells
DC.creator	Matsugaki, Aira
	Matsuzaka, Tadaaki
	Mori, Toko
	Saito, Mitsuka
	Funaoku, Kazuma
	Yamano, Riku
	Gokcekaya, Ozkan
	Ozasa, Ryosuke
	Nakano, Takayoshi
DC.publisher	AccScience Publishing
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-01-09
DC.identifier	https://doi.org/10.36922/ijb.1425
DC.subject	Additive manufacturing
	Laser powder bed fusion
	Mesenchymal stem
	Osteogenic differentiation
	Bone microstructure
DC.description	Matsugaki Aira, Matsuzaka Tadaaki, Mori Toko, et al. PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells. International Journal of Bioprinting 10, 1425 (2024); https://doi.org/10.36922/ijb.1425.
DCTERMS.abstract	Stem cell differentiation has important implications for biomedical device design and tissue engineering. Recently, inherent material properties, including surface chemistry, stiffness, and topography, have been found to influence stem cell fate. Among these, surface topography is a key regulator of stem cells in contact with materials. The most important aspect of ideal bone tissue engineering is to control the organization of the bone extracellular matrix with fully differentiated osteoblasts. Here, we found that laser powder bed fusion (PBF-LB)-fabricated grooved surface inspired by the microstructure of bone, which induced human mesenchymal stem cell (hMSC) differentiation into the osteogenic lineage without any differentiation supplements. The periodic grooved structure was fabricated by PBF-LB which induced cell elongation facilitated by cytoskeletal tension along the grooves. This resulted in the upregulation of osteogenesis via Runx2 expression. The aligned hMSCs successfully differentiated into osteoblasts and further organized the bone mimetic-oriented extracellular matrix microstructure. Our results indicate that metal additive manufacturing technology has a great advantage in controlling stem cell fate into the osteogenic lineage, and in the construction of bone-mimetic microstructural organization. Our findings on material-induced stem cell differentiation under standard cell culture conditions open new avenues for the development of medical devices that realize the desired tissue regeneration mediated by regulated stem cell functions.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93524
citation_title	PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells
citation_author	Matsugaki, Aira
	Matsuzaka, Tadaaki
	Mori, Toko
	Saito, Mitsuka
	Funaoku, Kazuma
	Yamano, Riku
	Gokcekaya, Ozkan
	Ozasa, Ryosuke
	Nakano, Takayoshi
citation_publisher	AccScience Publishing
citation_language	English
citation_date	2024-01-09
citation_journal_title	International Journal of Bioprinting
citation_volume	10
citation_issue	1
citation_firstpage	1425
citation_doi	https://doi.org/10.36922/ijb.1425
citation_public_url	https://hdl.handle.net/11094/93524

PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells

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