Disruption of a licorice cellulose synthase-derived glycosyltransferase gene demonstrates its in planta role in soyasaponin biosynthesis

Sakanishi, Manami; Chung, Soo Yeon; Fujiwara, Kentaro et al.

Plant Cell Reports, 2024, 43(15)

Number of Access:645（2025-10-19 13:32 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93546

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Title
Disruption of a licorice cellulose synthase-derived glycosyltransferase gene demonstrates its in planta role in soyasaponin biosynthesis
Creator
CreatorName
Sakanishi, Manami
CreatorName
Chung, Soo Yeon
CreatorName
Fujiwara, Kentaro
NRID
1000080342781 1000080342781
CreatorName
Kojoma, Mareshige
NRID
1000060342862 1000060342862
ORCID
0000-0003-1058-2473 0000-0003-1058-2473
researchmap permalink
bio bio
Scopus Author ID
7006488017 7006488017
CreatorName
Muranaka, Toshiya
NRID
1000030392004 1000030392004
ORCID
0000-0002-6426-0368 0000-0002-6426-0368
researchmap permalink
read0152753 read0152753
Scopus Author ID
7202492827 7202492827
CreatorName
Seki, Hikaru
Subject
Biosynthesis
Cellulose synthase-like
Genome editing
Glycosyltransferase
Licorice
Soyasaponin
Description
The version of record of this article, first published in Plant Cell Reports, is available online at Publisher’s website: https://doi.org/10.1007/s00299-023-03095-6
Abstract
Key message: CRISPR–Cas9-mediated disruption of a licorice cellulose synthase-derived glycosyltransferase gene, GuCSyGT, demonstrated the in planta role of GuCSyGT as the enzyme catalyzing 3-O-glucuronosylation of triterpenoid aglycones in soyasaponin biosynthesis. Abstract: Triterpenoid glycosides (saponins) are a large, structurally diverse group of specialized metabolites in plants, including the sweet saponin glycyrrhizin produced by licorice (Glycyrrhiza uralensis) and soyasaponins that occur widely in legumes, with various bioactivities. The triterpenoid saponin biosynthetic pathway involves the glycosylation of triterpenoid sapogenins (the non-sugar part of triterpenoid saponins) by glycosyltransferases (GTs), leading to diverse saponin structures. Previously, we identified a cellulose synthase-derived GT (CSyGT), as a newly discovered class of triterpenoid GT from G. uralensis. GuCSyGT expressed in yeast, which could transfer the sugar glucuronic acid to the C3 position of glycyrrhetinic acid and soyasapogenol B, which are the sapogenins of glycyrrhizin and soyasaponin I, respectively. This suggested that GuCSyGT is involved in the biosynthesis of glycyrrhizin and soyasaponin I. However, the in planta role of GuCSyGT in saponin biosynthesis remains unclear. In this study, we generated GuCSyGT-disrupted licorice hairy roots using CRISPR–Cas9-mediated genome editing and analyzed the saponin content. This revealed that soyasaponin I was completely absent in GuCSyGT-disrupted lines, demonstrating the in planta role of GuCSyGT in saponin biosynthesis.
Publisher
Springer Science and Business Media Deutschland GmbH
Source Title
Plant Cell Reports
Volume (Issue)
43(15)
Date of Issued
2024-01-01
Language
English
Handle URL
https://hdl.handle.net/11094/93546
PISSN
07217714
eISSN
1432203X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s00299-023-03095-6
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article