Effect of chronic intermittent hypoxia on ocular and intraoral mechanical allodynia mediated via the calcitonin gene-related peptide in a rat

Katagiri, Ayano; Kishimoto, Saki; Okamoto, Yoshie et al.

Sleep, 2024, 47(3), zsad332

Number of Access:702（2025-08-16 02:40 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93617

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Title
Effect of chronic intermittent hypoxia on ocular and intraoral mechanical allodynia mediated via the calcitonin gene-related peptide in a rat
Creator
NRID
1000040731899 1000040731899
ORCID
0000-0001-7381-8557 0000-0001-7381-8557
researchmap permalink
katagiri-ayano katagiri-ayano
Scopus Author ID
47161239400 47161239400
CreatorName
Katagiri, Ayano
CreatorName
Kishimoto, Saki
CreatorName
Okamoto, Yoshie
CreatorName
Yamada, Masaharu
NRID
1000030218250 1000030218250
researchmap permalink
19580710 19580710
Scopus Author ID
24351577000 24351577000
CreatorName
Niwa, Hitoshi
CreatorName
Bereiter, David A
NRID
1000050367520 1000050367520
ORCID
0000-0003-2452-7328 0000-0003-2452-7328
researchmap permalink
read0111598_hrfc15 read0111598_hrfc15
Scopus Author ID
57210579311 57210579311
CreatorName
Kato, Takafumi
Subject
rats
calcitonin gene-related peptide
neurons
orofacial pain
hypoxia
obstructive sleep apnea
Description
Katagiri Ayano, Kishimoto Saki, Okamoto Yoshie, et al. Effect of chronic intermittent hypoxia on ocular and intraoral mechanical allodynia mediated via the calcitonin gene-related peptide in a rat. SLEEP 7, 687 (2024); https://doi.org/10.1093/sleep/zsad332.
Abstract
Study Objectives: Obstructive sleep apnea, a significant hypoxic condition, may exacerbate several orofacial pain conditions. The study aims to define the involvement of calcitonin gene-related peptide (CGRP) in peripheral and central sensitization and in evoking orofacial mechanical allodynia under chronic intermittent hypoxia (CIH). Methods: Male rats were exposed to CIH. Orofacial mechanical allodynia was assessed using the eyeblink test and the two-bottle preference drinking test. The CGRP-immunoreactive neurons in the trigeminal ganglion (TG), CGRP-positive primary afferents projecting to laminae I–II of the trigeminal spinal subnucleus caudalis (Vc), and neural responses in the second-order neurons of the Vc were determined by immunohistochemistry. CGRP receptor antagonist was administrated in the TG. Results: CIH-induced ocular and intraoral mechanical allodynia. CGRP-immunoreactive neurons and activated satellite glial cells (SGCs) were significantly increased in the TG and the number of cFos-immunoreactive cells in laminae I–II of the Vc were significantly higher in CIH rats compared to normoxic rats. Local administration of the CGRP receptor antagonist in the TG of CIH rats attenuated orofacial mechanical allodynia; the number of CGRP-immunoreactive neurons and activated SGCs in the TG, and the density of CGRP-positive primary afferent terminals and the number of cFos-immunoreactive cells in laminae I–II of the Vc were significantly lower compared to vehicle-administrated CIH rats. Conclusions: An increase in CGRP in the TG induced by CIH, as well as orofacial mechanical allodynia and central sensitization of second-order neurons in the Vc, supported the notion that CGRP plays a critical role in CIH-induced orofacial mechanical allodynia.
Publisher
Oxford University Press (OUP)
Source Title
Sleep
Volume (Issue)
47(3)
Page
zsad332
Date of Issued
2024-01-02
Language
English
Handle URL
https://hdl.handle.net/11094/93617
PISSN
01618105
eISSN
15509109
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1093/sleep/zsad332
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
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Category
学術雑誌論文 Journal Article