Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia

Nakatani, Ayaka; Okumura, Ryu; Ishibashi, Airi; Okamoto, Shota; Sakaki, Kei; Ito, Yuki; Okuzaki, Daisuke; Inohara, Hidenori; Takeda, Kiyoshi

doi:https://doi.org/10.1111/gtc.13065

Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia

Nakatani, Ayaka; Okumura, Ryu; Ishibashi, Airi et al.

Genes to Cells, 2023, 28(11), 776-788

Number of Access:1,385（2025-11-02 06:23 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93980

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Title	Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia
Creator	NRID 1000080846395 1000080846395 researchmap permalink anakatani anakatani CreatorName Nakatani, Ayaka
	NRID 1000000793449 1000000793449 researchmap permalink ryoharei301324 ryoharei301324 Scopus Author ID 55176912300 55176912300 CreatorName Okumura, Ryu
	CreatorName Ishibashi, Airi
	CreatorName Okamoto, Shota
	CreatorName Sakaki, Kei
	CreatorName Ito, Yuki
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 CreatorName Okuzaki, Daisuke
	NRID 1000000273657 1000000273657 researchmap permalink read0046473 read0046473 Scopus Author ID 7004501837 7004501837 CreatorName Inohara, Hidenori
	NRID 1000020309446 1000020309446 ORCID 0000-0002-1778-6332 0000-0002-1778-6332 researchmap permalink read0118278 read0118278 Scopus Author ID 7404335442 7404335442 CreatorName Takeda, Kiyoshi
Subject	cell proliferation
	gut microbiota
	IL-23/IL-22 axis
	intestinal epithelial cells
	lipid metabolism
Description	This is the peer reviewed version of the following article: Nakatani A., Okumura R., Ishibashi A., et al. Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia. Genes to Cells 28, 776 (2023), which has been published in final form at https://doi.org/10.1111/gtc.13065. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Abstract	In the intestine, interleukin (IL)-23 and IL-22 from immune cells in the lamina propria contribute to maintenance of the gut epithelial barrier through the induction of antimicrobial production and the promotion of epithelial cell proliferation. Several previous studies suggested that some of the functions of the IL-23/IL-22 axis on intestinal epithelial cells are shared between the small and large intestines. However, the similarities and differences of the IL-23/IL-22 axis on epithelial cells between these two anatomical sites remain unclear. Here, we comprehensively analyzed the gene expression of intestinal epithelial cells in the ileum and colon of germ-free, Il23−/−, and Il22−/− mice by RNA-sequencing. We found that while the IL-23/IL-22 axis is largely dependent on gut microbiota in the small intestine, it is much less dependent on it in the large intestine. In addition, the negative regulation of lipid metabolism in the epithelial cells by IL-23 and IL-22 in the small intestine was revealed, whereas the positive regulation of epithelial cell proliferation by IL-23 and IL-22 in the large intestine was highlighted. These findings shed light on the intestinal site-specific role of the IL-23/IL-22 axis in maintaining the physiological functions of intestinal epithelial cells.
Publisher	John Wiley and Sons Inc
Source Title	Genes to Cells
Volume (Issue)	28(11)
Page	776-788
Date of Issued	2023-11-01
Language	English
Handle URL	https://hdl.handle.net/11094/93980
PISSN	13569597
eISSN	13652443
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1111/gtc.13065
Access Rights	open access
CopyRight	© 2023 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Genes to Cells. 2023, 28(11), p. 776-788
DC.title	Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia
DC.creator	Nakatani, Ayaka
	Okumura, Ryu
	Ishibashi, Airi
	Okamoto, Shota
	Sakaki, Kei
	Ito, Yuki
	Okuzaki, Daisuke
	Inohara, Hidenori
	Takeda, Kiyoshi
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-11-01
DC.identifier	https://doi.org/10.1111/gtc.13065
DC.subject	cell proliferation
	gut microbiota
	IL-23/IL-22 axis
	intestinal epithelial cells
	lipid metabolism
DC.description	This is the peer reviewed version of the following article: Nakatani A., Okumura R., Ishibashi A., et al. Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia. Genes to Cells 28, 776 (2023), which has been published in final form at https://doi.org/10.1111/gtc.13065. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
DCTERMS.abstract	In the intestine, interleukin (IL)-23 and IL-22 from immune cells in the lamina propria contribute to maintenance of the gut epithelial barrier through the induction of antimicrobial production and the promotion of epithelial cell proliferation. Several previous studies suggested that some of the functions of the IL-23/IL-22 axis on intestinal epithelial cells are shared between the small and large intestines. However, the similarities and differences of the IL-23/IL-22 axis on epithelial cells between these two anatomical sites remain unclear. Here, we comprehensively analyzed the gene expression of intestinal epithelial cells in the ileum and colon of germ-free, Il23−/−, and Il22−/− mice by RNA-sequencing. We found that while the IL-23/IL-22 axis is largely dependent on gut microbiota in the small intestine, it is much less dependent on it in the large intestine. In addition, the negative regulation of lipid metabolism in the epithelial cells by IL-23 and IL-22 in the small intestine was revealed, whereas the positive regulation of epithelial cell proliferation by IL-23 and IL-22 in the large intestine was highlighted. These findings shed light on the intestinal site-specific role of the IL-23/IL-22 axis in maintaining the physiological functions of intestinal epithelial cells.
DC.rights	© 2023 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
DC.format	93980
citation_title	Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia
citation_author	Nakatani, Ayaka
	Okumura, Ryu
	Ishibashi, Airi
	Okamoto, Shota
	Sakaki, Kei
	Ito, Yuki
	Okuzaki, Daisuke
	Inohara, Hidenori
	Takeda, Kiyoshi
citation_publisher	John Wiley and Sons Inc
citation_language	English
citation_date	2023-11-01
citation_journal_title	Genes to Cells
citation_volume	28
citation_issue	11
citation_firstpage	776
citation_lastpage	788
citation_doi	https://doi.org/10.1111/gtc.13065
citation_public_url	https://hdl.handle.net/11094/93980

Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia

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