Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts

Zhang, Jingbo; Li, Junjun; Qu, Xiang et al.

Stem Cell Research and Therapy, 2023, 14, 380

Number of Access:1,181（2025-08-15 10:59 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/94588

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Title
Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts
Creator
CreatorName
Zhang, Jingbo
NRID
1000010723786 1000010723786
ORCID
0000-0002-7473-4918 0000-0002-7473-4918
researchmap permalink
liJJ liJJ
Scopus Author ID
52464043600 52464043600
CreatorName
Li, Junjun
CreatorName
Qu, Xiang
CreatorName
Liu, Yuting
NRID
1000070535426 1000070535426
CreatorName
Harada, Akima
CreatorName
Hua, Ying
CreatorName
Yoshida, Noriko
CreatorName
Ishida, Masako
CreatorName
Tabata, Akiko
CreatorName
Sun, Lifu
NRID
1000050380093 1000050380093
researchmap permalink
7000008694 7000008694
Scopus Author ID
55628530013 55628530013
CreatorName
Liu, Li
NRID
1000070544237 1000070544237
ORCID
0000-0003-0015-6569 0000-0003-0015-6569
researchmap permalink
s-miyagawa3154 s-miyagawa3154
Scopus Author ID
55560624700 55560624700
CreatorName
Miyagawa, Shigeru
Subject
Adipose-derived mesenchymal stem cell
Angiogenesis
Myocardial infarction
Regenerative medicine
Tissue engineering
Description
Zhang J., Li J., Qu X., et al. Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts. Stem Cell Research and Therapy 14, 380 (2023); https://doi.org/10.1186/s13287-023-03560-9.
Abstract
Background: Heart failure (HF) is a major cause of death worldwide. The most effective treatment for HF is heart transplantation, but its use is limited by the scarcity of donor hearts. Recently, stem cell-based therapy has emerged as a promising approach for treating myocardial infarction. Our research group has been investigating the use of human induced pluripotent stem cell-derived cardiomyocyte patches as a potential therapeutic candidate. We have successfully conducted eight cases of clinical trials and demonstrated the safety and effectiveness of this approach. However, further advancements are necessary to overcome immune rejection and enhance therapeutic efficacy. In this study, we propose a novel and efficient technique for constructing mesenchymal stem cell (MSC) tissue sheets, which can be transplanted effectively for treating myocardial infarction repair. Methods: We applied a one-step method to construct the human adipose-derived mesenchymal stem cell (hADSC) tissue sheet on a poly(lactic-co-glycolic acid) fiber scaffold. Histology, immunofluorescence, and paracrine profile assessment were used to determine the organization and function of the hADSC tissue sheet. Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function, fibrosis area, angiogenesis, and left ventricular remodeling. Results: In vitro, the hADSC tissue sheet showed great organization, abundant ECM expression, and increased paracrine secretion than single cells. In vivo, the hADSC tissue sheet group demonstrated improved cardiac functional recovery, less ventricular remodeling, decreased fibrosis, and enhanced angiogenesis than the MI group. Conclusions: We developed thick and functional hADSC tissue sheets via the one-step strategy. The hADSC tissue sheet showed excellent performance in treating myocardial infarction in the rat model.
Publisher
BioMed Central Ltd
Source Title
Stem Cell Research and Therapy
Volume (Issue)
14
Page
380
Date of Issued
2023-12-20
Language
English
Handle URL
https://hdl.handle.net/11094/94588
eISSN
17576512
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1186/s13287-023-03560-9
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article