Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

Li, Junjun; Hua, Ying; Liu, Yuting; Qu, Xiang; Zhang, Jingbo; Ishida, Masako; Yoshida, Noriko; Tabata, Akiko; Miyoshi, Hayato; Shiba, Mikio; Higo, Shuichiro; Sougawa, Nagako; Takeda, Maki; Kawamura, Takuji; Matsuura, Ryohei; Okuzaki, Daisuke; Toyofuku, Toshihiko; Sawa, Yoshiki; Liu, Li; Miyagawa, Shigeru

doi:https://doi.org/10.1016/j.isci.2024.108992

Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

Li, Junjun; Hua, Ying; Liu, Yuting et al.

iScience, 2024, 27(2), 108992

Number of Access:2,444（2025-07-18 09:48 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/94598

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Title	Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
Creator	NRID 1000010723786 1000010723786 ORCID 0000-0002-7473-4918 0000-0002-7473-4918 researchmap permalink liJJ liJJ Scopus Author ID 52464043600 52464043600 CreatorName Li, Junjun
	CreatorName Hua, Ying
	CreatorName Liu, Yuting
	CreatorName Qu, Xiang
	CreatorName Zhang, Jingbo
	CreatorName Ishida, Masako
	CreatorName Yoshida, Noriko
	CreatorName Tabata, Akiko
	CreatorName Miyoshi, Hayato
	CreatorName Shiba, Mikio
	NRID 1000000604034 1000000604034 researchmap permalink s.higo s.higo Scopus Author ID 23018633200 23018633200 CreatorName Higo, Shuichiro
	NRID 1000030432579 1000030432579 researchmap permalink SougawaNagako SougawaNagako CreatorName Sougawa, Nagako
	CreatorName Takeda, Maki
	NRID 1000060839398 1000060839398 researchmap permalink takuji-kawamura takuji-kawamura Scopus Author ID 55331768500 55331768500 ORCID 0000-0003-2985-0036 0000-0003-2985-0036 CreatorName Kawamura, Takuji
	NRID 1000010768922 1000010768922 researchmap permalink ryoheimatsuura ryoheimatsuura Scopus Author ID 57195988109 57195988109 CreatorName Matsuura, Ryohei
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 CreatorName Okuzaki, Daisuke
	NRID 1000060322179 1000060322179 CreatorName Toyofuku, Toshihiko
	NRID 1000000243220 1000000243220 researchmap permalink y-sawa0703 y-sawa0703 Scopus Author ID 55428162700 55428162700 CreatorName Sawa, Yoshiki
	NRID 1000050380093 1000050380093 researchmap permalink 7000008694 7000008694 Scopus Author ID 55628530013 55628530013 CreatorName Liu, Li
	NRID 1000070544237 1000070544237 ORCID 0000-0003-0015-6569 0000-0003-0015-6569 researchmap permalink s-miyagawa3154 s-miyagawa3154 Scopus Author ID 55560624700 55560624700 CreatorName Miyagawa, Shigeru
Subject	Bioengineering
	Biotechnology
	Cardiovascular medicine
	Stem cells research
Description	Li J., Hua Y., Liu Y., et al. Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment. iScience 27, 108992 (2024); https://doi.org/10.1016/j.isci.2024.108992.
Abstract	Human iPSC-derived cardiomyocytes (hiPSC-CMs) exhibit functional immaturity, potentially impacting their suitability for assessing drug proarrhythmic potential. We previously devised a traveling wave (TW) system to promote maturation in 3D cardiac tissue. To align with current drug assessment paradigms (CiPA and JiCSA), necessitating a 2D monolayer cardiac tissue, we integrated the TW system with a multi-electrode array. This gave rise to a hiPSC-derived closed-loop cardiac tissue (iCT), enabling spontaneous TW initiation and swift pacing of cardiomyocytes from various cell lines. The TW-paced cardiomyocytes demonstrated heightened sarcomeric and functional maturation, exhibiting enhanced response to isoproterenol. Moreover, these cells showcased diminished sensitivity to verapamil and maintained low arrhythmia rates with ranolazine—two drugs associated with a low risk of torsades de pointes (TdP). Notably, the TW group displayed increased arrhythmia rates with high and intermediate risk TdP drugs (quinidine and pimozide), underscoring the potential utility of this system in drug assessment applications.
Publisher	Elsevier Inc.
Source Title	iScience
Volume (Issue)	27(2)
Page	108992
Date of Issued	2024-02-16
Language	English
Handle URL	https://hdl.handle.net/11094/94598
eISSN	25890042
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.isci.2024.108992
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	iScience. 2024, 27(2), p. 108992
DC.title	Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
DC.creator	Li, Junjun
	Hua, Ying
	Liu, Yuting
	Qu, Xiang
	Zhang, Jingbo
	Ishida, Masako
	Yoshida, Noriko
	Tabata, Akiko
	Miyoshi, Hayato
	Shiba, Mikio
	Higo, Shuichiro
	Sougawa, Nagako
	Takeda, Maki
	Kawamura, Takuji
	Matsuura, Ryohei
	Okuzaki, Daisuke
	Toyofuku, Toshihiko
	Sawa, Yoshiki
	Liu, Li
	Miyagawa, Shigeru
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-02-16
DC.identifier	https://doi.org/10.1016/j.isci.2024.108992
DC.subject	Bioengineering
	Biotechnology
	Cardiovascular medicine
	Stem cells research
DC.description	Li J., Hua Y., Liu Y., et al. Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment. iScience 27, 108992 (2024); https://doi.org/10.1016/j.isci.2024.108992.
DCTERMS.abstract	Human iPSC-derived cardiomyocytes (hiPSC-CMs) exhibit functional immaturity, potentially impacting their suitability for assessing drug proarrhythmic potential. We previously devised a traveling wave (TW) system to promote maturation in 3D cardiac tissue. To align with current drug assessment paradigms (CiPA and JiCSA), necessitating a 2D monolayer cardiac tissue, we integrated the TW system with a multi-electrode array. This gave rise to a hiPSC-derived closed-loop cardiac tissue (iCT), enabling spontaneous TW initiation and swift pacing of cardiomyocytes from various cell lines. The TW-paced cardiomyocytes demonstrated heightened sarcomeric and functional maturation, exhibiting enhanced response to isoproterenol. Moreover, these cells showcased diminished sensitivity to verapamil and maintained low arrhythmia rates with ranolazine—two drugs associated with a low risk of torsades de pointes (TdP). Notably, the TW group displayed increased arrhythmia rates with high and intermediate risk TdP drugs (quinidine and pimozide), underscoring the potential utility of this system in drug assessment applications.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	94598
citation_title	Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
citation_author	Li, Junjun
	Hua, Ying
	Liu, Yuting
	Qu, Xiang
	Zhang, Jingbo
	Ishida, Masako
	Yoshida, Noriko
	Tabata, Akiko
	Miyoshi, Hayato
	Shiba, Mikio
	Higo, Shuichiro
	Sougawa, Nagako
	Takeda, Maki
	Kawamura, Takuji
	Matsuura, Ryohei
	Okuzaki, Daisuke
	Toyofuku, Toshihiko
	Sawa, Yoshiki
	Liu, Li
	Miyagawa, Shigeru
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2024-02-16
citation_journal_title	iScience
citation_volume	27
citation_issue	2
citation_firstpage	108992
citation_doi	https://doi.org/10.1016/j.isci.2024.108992
citation_public_url	https://hdl.handle.net/11094/94598

Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

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