Transcriptomic analysis of Porphyromonas gingivalis-infected head and neck cancer cells: Identification of PLAU as a candidate prognostic biomarker

Hamada, Masakazu; Inaba, Hiroaki; Nishiyama, Kyoko et al.

Journal of Cellular and Molecular Medicine, 2024, 28(4), JCMM18167

Number of Access:716（2025-07-24 20:36 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/95670

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Title
Transcriptomic analysis of Porphyromonas gingivalis-infected head and neck cancer cells: Identification of PLAU as a candidate prognostic biomarker
Creator
NRID
1000080506361 1000080506361
researchmap permalink
6361 6361
Scopus Author ID
36616148000 36616148000
CreatorName
Hamada, Masakazu
NRID
1000070359850 1000070359850
CreatorName
Inaba, Hiroaki
NRID
1000070846542 1000070846542
researchmap permalink
02205 02205
CreatorName
Nishiyama, Kyoko
NRID
1000040801048 1000040801048
CreatorName
Yoshida, Sho
NRID
1000000136277 1000000136277
CreatorName
Yura, Yoshiaki
NRID
1000030359848 1000030359848
CreatorName
Matsumoto-Nakano, Michiyo
NRID
1000030345285 1000030345285
researchmap permalink
read0212957 read0212957
Scopus Author ID
6603144046 6603144046
CreatorName
Uzawa, Narikazu
Subject
head and neck squamous cell carcinoma
migration
P. gingivalis
PLAU
RNA sequencing
TCGA
Description
Hamada M., Inaba H., Nishiyama K., et al. Transcriptomic analysis of Porphyromonas gingivalis-infected head and neck cancer cells: Identification of PLAU as a candidate prognostic biomarker. Journal of Cellular and Molecular Medicine 28, JCMM18167 (2024); https://doi.org/10.1111/jcmm.18167.
Abstract
Periodontal disease is a risk factor for head and neck squamous cell carcinoma (HNSCC), and Porphyromonas gingivalis, a major periodontal pathogen, has been identified as a specific and potentially independent microbial factor that increases the risk of cancer mortality. Gene expression in HNSCC due to P. gingivalis infection and how changes in gene expression affect the prognosis of HNSCC patients are not clarified. When P. gingivalis was cultured with HNSCC cells, it efficiently adhered to these cells and enhanced their invasive ability. A transcriptome analysis of P. gingivalis -infected HNSCC cells showed that genes related to migration, including CCL20, CITED2, CTGF, C8orf44-SGK3, DUSP10, EGR3, FUZ, HBEGF, IL1B, IL24, JUN, PLAU, PTGS2, P2RY1, SEMA7A, SGK1 and SIX2, were highly up- or down-regulated. The expression of up-regulated genes was examined using the expression data of HNSCC patients obtained from The Cancer Genome Atlas (TCGA) database, and the expression of 5 genes, including PLAU, was found to be higher in cancer tissue than in solid normal tissue. An analysis of protein–protein interactions revealed that these 5 genes formed a dense network. A Cox regression analysis showed that high PLAU expression levels were associated with a poor prognosis in patients with TCGA-HNSCC. Furthermore, the prognostic impact correlated with tumour size and the presence or absence of lymph node metastasis. Collectively, these results suggest the potential of PLAU as a molecular prognostic marker in HNSCC patients. Further in vivo and in vitro studies are needed to verify the findings of this study.
Publisher
John Wiley and Sons Inc
Source Title
Journal of Cellular and Molecular Medicine
Volume (Issue)
28(4)
Page
JCMM18167
Date of Issued
2024-02-16
Language
English
Handle URL
https://hdl.handle.net/11094/95670
PISSN
15821838
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1111/jcmm.18167
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
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Version of Record
Category
学術雑誌論文 Journal Article