Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo

Ichimura, Taro; Kakizuka, Taishi; Sato, Yuki; Itano, Keiko; Seiriki, Kaoru; Hashimoto, Hitoshi; Itoga, Hiroya; Onami, Shuichi; Nagai, Takeharu

doi:https://doi.org/10.7554/eLife.93633

Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo

Ichimura, Taro; Kakizuka, Taishi; Sato, Yuki et al.

eLife, 2024, 493(6), 318

Number of Access:973（2025-07-27 03:40 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/95676

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Title	Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Creator	NRID 1000050600748 1000050600748 ORCID 0000-0002-3740-3634 0000-0002-3740-3634 researchmap permalink taroichimura taroichimura Scopus Author ID 7103390896 7103390896 CreatorName Ichimura, Taro
	CreatorName Kakizuka, Taishi
	NRID 1000090508186 1000090508186 CreatorName Sato, Yuki
	CreatorName Itano, Keiko
	NRID 1000090802918 1000090802918 researchmap permalink kaoru-seiriki kaoru-seiriki Scopus Author ID 55318807200 55318807200 CreatorName Seiriki, Kaoru
	NRID 1000030240849 1000030240849 researchmap permalink hitoshi-hashimoto hitoshi-hashimoto Scopus Author ID 35352982100 35352982100 ORCID 0000-0001-6548-4016 0000-0001-6548-4016 CreatorName Hashimoto, Hitoshi
	NRID 1000000373100 1000000373100 CreatorName Itoga, Hiroya
	NRID 1000050348843 1000050348843 CreatorName Onami, Shuichi
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 CreatorName Nagai, Takeharu
Description	Ichimura Taro, Kakizuka Taishi, Sato Yuki, et al. Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo. eLife 493, 318 (2024); https://doi.org/10.7554/eLife.93633.
Abstract	We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of 2× and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a lateral spatial resolution of approximately 1.2 µm across an FOV of approximately 1.5 × 1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hours, visualizing the movement of over 4.0 × 105 vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.
Publisher	eLife Sciences Publications, Ltd
Source Title	eLife
Volume (Issue)	493(6)
Page	318
Date of Issued	2024-02-28
Language	English
Handle URL	https://hdl.handle.net/11094/95676
eISSN	2050084X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.7554/eLife.93633
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	eLife. 2024, 493(6), p. 318
DC.title	Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
DC.creator	Ichimura, Taro
	Kakizuka, Taishi
	Sato, Yuki
	Itano, Keiko
	Seiriki, Kaoru
	Hashimoto, Hitoshi
	Itoga, Hiroya
	Onami, Shuichi
	Nagai, Takeharu
DC.publisher	eLife Sciences Publications, Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-02-28
DC.identifier	https://doi.org/10.7554/eLife.93633
DC.description	Ichimura Taro, Kakizuka Taishi, Sato Yuki, et al. Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo. eLife 493, 318 (2024); https://doi.org/10.7554/eLife.93633.
DCTERMS.abstract	We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of 2× and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a lateral spatial resolution of approximately 1.2 µm across an FOV of approximately 1.5 × 1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hours, visualizing the movement of over 4.0 × 105 vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	95676
citation_title	Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
citation_author	Ichimura, Taro
	Kakizuka, Taishi
	Sato, Yuki
	Itano, Keiko
	Seiriki, Kaoru
	Hashimoto, Hitoshi
	Itoga, Hiroya
	Onami, Shuichi
	Nagai, Takeharu
citation_publisher	eLife Sciences Publications, Ltd
citation_language	English
citation_date	2024-02-28
citation_journal_title	eLife
citation_volume	493
citation_issue	6
citation_firstpage	318
citation_doi	https://doi.org/10.7554/eLife.93633
citation_public_url	https://hdl.handle.net/11094/95676

Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo

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