Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches

Ikuno, Yudai; Tsujino, Hirofumi; Haga, Yuya; Manabe, Sota; Idehara, Wakaba; Hokaku, Mii; Asahara, Haruyasu; Higashisaka, Kazuma; Tsutsumi, Yasuo

doi:https://doi.org/10.1016/j.ecoenv.2024.116346

Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches

Ikuno, Yudai; Tsujino, Hirofumi; Haga, Yuya et al.

Ecotoxicology and Environmental Safety, 2024, 277, 116346

Number of Access:1,034（2025-07-23 00:53 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/95779

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
EcotoxicolEnvironSaf_277_116346	pdf	None	7.65 MB	189	2024.05.31

Creative Commons : Attribution - NonCommercial - NoDerivatives

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches
Creator	CreatorName Ikuno, Yudai
	NRID 1000010707144 1000010707144 researchmap permalink htsujino htsujino Scopus Author ID 36667726200 36667726200 CreatorName Tsujino, Hirofumi
	NRID 1000030888863 1000030888863 ORCID 0000-0003-4148-2256 0000-0003-4148-2256 researchmap permalink 30888863 30888863 Scopus Author ID 57211023415 57211023415 CreatorName Haga, Yuya
	CreatorName Manabe, Sota
	CreatorName Idehara, Wakaba
	CreatorName Hokaku, Mii
	NRID 1000020632318 1000020632318 ORCID 0000-0002-1808-7373 0000-0002-1808-7373 researchmap permalink pikurin3 pikurin3 Scopus Author ID 8856988200 8856988200 CreatorName Asahara, Haruyasu
	NRID 1000020646757 1000020646757 researchmap permalink read646757 read646757 Scopus Author ID 35603386200 35603386200 CreatorName Higashisaka, Kazuma
	NRID 1000050263306 1000050263306 researchmap permalink ytsutsumi ytsutsumi Scopus Author ID 7202474093 7202474093 CreatorName Tsutsumi, Yasuo
Subject	Cytotoxicity
	Microplastic degradation
	Microplastics
	Polyethylene
	Vacuum ultraviolet irradiation
Description	Ikuno Y., Tsujino H., Haga Y., et al. Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches. Ecotoxicology and Environmental Safety 277, 116346 (2024); https://doi.org/10.1016/j.ecoenv.2024.116346.
Abstract	Microplastics, plastic particles 5 mm or less in size, are abundant in the environment; hence, the exposure of humans to microplastics is a great concern. Usually, the surface of microplastics found in the environment has undergone degradation by external factors such as ultraviolet rays and water waves. One of the characteristics of changes caused by surface degradation of microplastics is the introduction of oxygen-containing functional groups. Surface degradation alters the physicochemical properties of plastics, suggesting that the biological effects of environmentally degraded plastics may differ from those of pure plastics. However, the biological effects of plastics introduced with oxygen-containing functional groups through degradation are poorly elucidated owing to the lack of a plastic sample that imitates the degradation state of plastics found in the environment. In this study, we investigated the degradation state of microplastics collected from a beach. Next, we degraded a commercially available polyethylene (PE) particles via vacuum ultraviolet (VUV) irradiation and showed that chemical surface state of PE imitates that of microplastics in the environment. We evaluated the cytotoxic effects of degraded PE samples on immune and epithelial cell lines. We found that VUV irradiation was effective in degrading PE within a short period, and concentration-dependent cytotoxicity was induced by degraded PE in all cell lines. Our results indicate that the cytotoxic effect of PE on different cell types depends on the degree of microplastic degradation, which contributes to our understanding of the effects of PE microplastics on humans.
Publisher	Elsevier
Source Title	Ecotoxicology and Environmental Safety
Volume (Issue)	277
Page	116346
Date of Issued	2024-06-01
Language	English
Handle URL	https://hdl.handle.net/11094/95779
PISSN	01476513
eISSN	10902414
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.ecoenv.2024.116346
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Ecotoxicology and Environmental Safety. 2024, 277, p. 116346
DC.title	Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches
DC.creator	Ikuno, Yudai
	Tsujino, Hirofumi
	Haga, Yuya
	Manabe, Sota
	Idehara, Wakaba
	Hokaku, Mii
	Asahara, Haruyasu
	Higashisaka, Kazuma
	Tsutsumi, Yasuo
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-06-01
DC.identifier	https://doi.org/10.1016/j.ecoenv.2024.116346
DC.subject	Cytotoxicity
	Microplastic degradation
	Microplastics
	Polyethylene
	Vacuum ultraviolet irradiation
DC.description	Ikuno Y., Tsujino H., Haga Y., et al. Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches. Ecotoxicology and Environmental Safety 277, 116346 (2024); https://doi.org/10.1016/j.ecoenv.2024.116346.
DCTERMS.abstract	Microplastics, plastic particles 5 mm or less in size, are abundant in the environment; hence, the exposure of humans to microplastics is a great concern. Usually, the surface of microplastics found in the environment has undergone degradation by external factors such as ultraviolet rays and water waves. One of the characteristics of changes caused by surface degradation of microplastics is the introduction of oxygen-containing functional groups. Surface degradation alters the physicochemical properties of plastics, suggesting that the biological effects of environmentally degraded plastics may differ from those of pure plastics. However, the biological effects of plastics introduced with oxygen-containing functional groups through degradation are poorly elucidated owing to the lack of a plastic sample that imitates the degradation state of plastics found in the environment. In this study, we investigated the degradation state of microplastics collected from a beach. Next, we degraded a commercially available polyethylene (PE) particles via vacuum ultraviolet (VUV) irradiation and showed that chemical surface state of PE imitates that of microplastics in the environment. We evaluated the cytotoxic effects of degraded PE samples on immune and epithelial cell lines. We found that VUV irradiation was effective in degrading PE within a short period, and concentration-dependent cytotoxicity was induced by degraded PE in all cell lines. Our results indicate that the cytotoxic effect of PE on different cell types depends on the degree of microplastic degradation, which contributes to our understanding of the effects of PE microplastics on humans.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	95779
citation_title	Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches
citation_author	Ikuno, Yudai
	Tsujino, Hirofumi
	Haga, Yuya
	Manabe, Sota
	Idehara, Wakaba
	Hokaku, Mii
	Asahara, Haruyasu
	Higashisaka, Kazuma
	Tsutsumi, Yasuo
citation_publisher	Elsevier
citation_language	English
citation_date	2024-06-01
citation_journal_title	Ecotoxicology and Environmental Safety
citation_volume	277
citation_firstpage	116346
citation_doi	https://doi.org/10.1016/j.ecoenv.2024.116346
citation_public_url	https://hdl.handle.net/11094/95779

Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches

Link to primary information

Item Information

Show details