Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens

Matsuzaki, Takahisa; Kawamura, Ryuzo; Yamamoto, Akihisa; Takahashi, Hozumi; Fujii, Mai; Togo, Shodai; Yoneyama, Yosuke; Hakuno, Fumihiko; Takahashi, Shin-Ichiro; Suganuma, Masami; Nakabayashi, Seiichiro; Sharma, Shivani; Gimzewski, James K.; Yoshikawa, Hiroshi Y.

doi:https://doi.org/10.1021/acs.jpclett.3c02767

Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens

Matsuzaki, Takahisa; Kawamura, Ryuzo; Yamamoto, Akihisa et al.

Journal of Physical Chemistry Letters, 2024, 15(4), 1097-1104

Number of Access:1,756（2025-07-31 23:24 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/96425

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Title	Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens
Creator	NRID 1000050830527 1000050830527 ORCID 0000-0001-9466-7495 0000-0001-9466-7495 researchmap permalink 7000026401 7000026401 Scopus Author ID 55317252000 55317252000 CreatorName Matsuzaki, Takahisa
	NRID 1000050534591 1000050534591 CreatorName Kawamura, Ryuzo
	NRID 1000090706805 1000090706805 CreatorName Yamamoto, Akihisa
	CreatorName Takahashi, Hozumi
	CreatorName Fujii, Mai
	CreatorName Togo, Shodai
	NRID 1000010748289 1000010748289 researchmap permalink yyoneyama yyoneyama CreatorName Yoneyama, Yosuke
	NRID 1000030282700 1000030282700 CreatorName Hakuno, Fumihiko
	NRID 1000000197146 1000000197146 CreatorName Takahashi, Shin-Ichiro
	NRID 1000020196695 1000020196695 CreatorName Suganuma, Masami
	NRID 1000070180346 1000070180346 CreatorName Nakabayashi, Seiichiro
	CreatorName Sharma, Shivani
	CreatorName Gimzewski, James K.
	NRID 1000050551173 1000050551173 ORCID 0000-0003-0624-6039 0000-0003-0624-6039 researchmap permalink read0108058 read0108058 Scopus Author ID 55708129100 55708129100 CreatorName Yoshikawa, Hiroshi Y.
Subject	Interference reflection microscopy
	Structured illumination
	Crystal growth
	Microtubule fiber network
	Cell Adhesion
Description	Matsuzaki T., Kawamura R., Yamamoto A., et al. Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens. Journal of Physical Chemistry Letters 15, 1097 (2024); https://doi.org/10.1021/acs.jpclett.3c02767.
Abstract	Interference reflection microscopy (IRM) is a powerful, label-free technique to visualize the surface structure of biospecimens. However, stray light outside a focal plane obscures the surface fine structures beyond the diffraction limit (dxy ≈ 200 nm). Here, we developed an advanced interferometry approach to visualize the surface fine structure of complex biospecimens, ranging from protein assemblies to single cells. Compared to 2-D, our unique 3-D structure illumination introduced to IRM enabled successful visualization of fine structures and the dynamics of protein crystal growth under lateral (dx-y ≈ 110 nm) and axial (dx-z ≤ 5 nm) resolutions and dynamical adhesion of microtubule fiber networks with lateral resolution (dx-y ≈ 120 nm), 10 times greater than unstructured IRM (dx-y ≈ 1000 nm). Simultaneous reflection/fluorescence imaging provides new physical fingerprints for studying complex biospecimens and biological processes such as myogenic differentiation and highlights the potential use of advanced interferometry to study key nanostructures of complex biospecimens.
Publisher	American Chemical Society
Source Title	Journal of Physical Chemistry Letters
Volume (Issue)	15(4)
Page	1097-1104
Date of Issued	2024-02-01
Language	English
Handle URL	https://hdl.handle.net/11094/96425
eISSN	19487185
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1021/acs.jpclett.3c02767
Access Rights	open access
CopyRight	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry Letters, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpclett.3c02767.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Journal of Physical Chemistry Letters. 2024, 15(4), p. 1097-1104
DC.title	Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens
DC.creator	Matsuzaki, Takahisa
	Kawamura, Ryuzo
	Yamamoto, Akihisa
	Takahashi, Hozumi
	Fujii, Mai
	Togo, Shodai
	Yoneyama, Yosuke
	Hakuno, Fumihiko
	Takahashi, Shin-Ichiro
	Suganuma, Masami
	Nakabayashi, Seiichiro
	Sharma, Shivani
	Gimzewski, James K.
	Yoshikawa, Hiroshi Y.
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-02-01
DC.identifier	https://doi.org/10.1021/acs.jpclett.3c02767
DC.subject	Interference reflection microscopy
	Structured illumination
	Crystal growth
	Microtubule fiber network
	Cell Adhesion
DC.description	Matsuzaki T., Kawamura R., Yamamoto A., et al. Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens. Journal of Physical Chemistry Letters 15, 1097 (2024); https://doi.org/10.1021/acs.jpclett.3c02767.
DCTERMS.abstract	Interference reflection microscopy (IRM) is a powerful, label-free technique to visualize the surface structure of biospecimens. However, stray light outside a focal plane obscures the surface fine structures beyond the diffraction limit (dxy ≈ 200 nm). Here, we developed an advanced interferometry approach to visualize the surface fine structure of complex biospecimens, ranging from protein assemblies to single cells. Compared to 2-D, our unique 3-D structure illumination introduced to IRM enabled successful visualization of fine structures and the dynamics of protein crystal growth under lateral (dx-y ≈ 110 nm) and axial (dx-z ≤ 5 nm) resolutions and dynamical adhesion of microtubule fiber networks with lateral resolution (dx-y ≈ 120 nm), 10 times greater than unstructured IRM (dx-y ≈ 1000 nm). Simultaneous reflection/fluorescence imaging provides new physical fingerprints for studying complex biospecimens and biological processes such as myogenic differentiation and highlights the potential use of advanced interferometry to study key nanostructures of complex biospecimens.
DC.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry Letters, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpclett.3c02767.
DC.format	96425
citation_title	Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens
citation_author	Matsuzaki, Takahisa
	Kawamura, Ryuzo
	Yamamoto, Akihisa
	Takahashi, Hozumi
	Fujii, Mai
	Togo, Shodai
	Yoneyama, Yosuke
	Hakuno, Fumihiko
	Takahashi, Shin-Ichiro
	Suganuma, Masami
	Nakabayashi, Seiichiro
	Sharma, Shivani
	Gimzewski, James K.
	Yoshikawa, Hiroshi Y.
citation_publisher	American Chemical Society
citation_language	English
citation_date	2024-02-01
citation_journal_title	Journal of Physical Chemistry Letters
citation_volume	15
citation_issue	4
citation_firstpage	1097
citation_lastpage	1104
citation_doi	https://doi.org/10.1021/acs.jpclett.3c02767
citation_public_url	https://hdl.handle.net/11094/96425

Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens

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