Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

Ren, Yu; Inoue, Daisuke; Ike, Michihiko

Journal of Material Cycles and Waste Management, 2024, 26, 2355-2365

Number of Access:299（2025-07-31 22:33 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97121

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Title
Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates
Creator
CreatorName
Ren, Yu
NRID
1000070448091 1000070448091
ORCID
0000-0002-9216-8198 0000-0002-9216-8198
researchmap permalink
DaisukeINOUE DaisukeINOUE
Scopus Author ID
55723196300 55723196300
CreatorName
Inoue, Daisuke
NRID
1000040222856 1000040222856
ORCID
0000-0001-6237-8103 0000-0001-6237-8103
researchmap permalink
ikemichi ikemichi
Scopus Author ID
55551254800 55551254800
CreatorName
Ike, Michihiko
Subject
Activated sludge
Aerobic dynamic discharge process
Mixed microbial culture
Polyhydroxyalkanoates
Substrate versatility
Description
The version of record of this article, first published in Journal of Material Cycles and Waste Management, is available online at Publisher’s website: https://doi.org/10.1007/s10163-024-01974-y.
Abstract
The use of waste activated sludge (WAS) as a biocatalyst to produce polyhydroxyalkanoates (PHA) from waste streams may help promote the beneficial use of WAS for low-carbon, sustainable wastewater treatment. However, it remains unclear which types of substrates can be used for efficient PHA production, and how the PHA production can be maximized. This study aimed to assess the substrate versatility of mixed microbial cultures (MMCs) constructed from WAS by enriching PHA-accumulating bacteria using an aerobic dynamic discharge (ADD) process fed with acetate. Twelve different substrates, including organic acids, saccharides, and alcohols, were selected as the test substrates. In single-batch assays, the highest PHA production (583–680 mg/L) was achieved using butyrate, acetate, and pyruvate. In fed-batch assays, > 30 wt% PHA content was achieved using acetate, butyrate, propionate, lactate, and ethanol, with the highest content (60.3 wt%) using acetate. These results indicate that acetate-fed MMC by the ADD process could efficiently produce PHA from volatile fatty acids, lactate, pyruvate, and ethanol. Polyhydroxybutyrate was preferentially produced from acetate, butyrate, pyruvate, lactate, and ethanol, whereas polyhydroxyvalerate was notably produced from propionate. The results suggest that PHA can be efficiently produced from a wide range of substrates using MMCs enriched on a single substrate.
Publisher
Springer
Source Title
Journal of Material Cycles and Waste Management
Volume (Issue)
26
Page
2355-2365
Date of Issued
2024-01-01
Language
English
Handle URL
https://hdl.handle.net/11094/97121
PISSN
14384957
eISSN
16118227
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s10163-024-01974-y
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article