Phototuning of Hyaluronic-Acid-Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells

Elvitigala, Kelum Chamara Manoj Lakmal; Mohan, Lakshmi; Mubarok, Wildan et al.

Advanced Healthcare Materials, 2024, 13(17), 2303787

Number of Access:478（2025-09-17 08:09 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97143

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Title
Phototuning of Hyaluronic-Acid-Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells
Creator
CreatorName
Elvitigala, Kelum Chamara Manoj Lakmal
CreatorName
Mohan, Lakshmi
CreatorName
Mubarok, Wildan
NRID
1000020359938 1000020359938
ORCID
0000-0002-1041-4798 0000-0002-1041-4798
researchmap permalink
sakaishinji sakaishinji
Scopus Author ID
35450082900 35450082900
CreatorName
Sakai, Shinji
Subject
composite hydrogel
degradation
HUVEC
hyaluronic acid
photo-crosslinking
Description
Elvitigala K.C.M.L., Mohan L., Mubarok W., et al. Phototuning of Hyaluronic-Acid-Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells. Advanced Healthcare Materials , (2024); https://doi.org/10.1002/adhm.202303787.
Abstract
In vitro network formation by endothelial cells serves as a fundamental model for studies aimed at understanding angiogenesis. The morphogenesis of these cells to form a network is intricately regulated by the mechanical and biochemical properties of the extracellular matrix. Here the effects of modulating these properties in hydrogels derived from phenolated hyaluronic acid (HA-Ph) and phenolated gelatin (Gelatin-Ph) are presented. Visible-light irradiation in the presence of tris(2,2′-bipyridyl)ruthenium(II) chloride hexahydrate and sodium persulfate induces the crosslinking of these polymers, thereby forming a hydrogel and degrading HA-Ph. Human vascular endothelial cells form networks on the hydrogel prepared by visible-light irradiation for 45 min (42 W cm−2 at 450 nm) but not on the hydrogels prepared by irradiation for 15, 30, or 60 min. The irradiation time-dependent degradation of HA-Ph and the changes in the mechanical stiffness of the hydrogels, coupled with the expressions of RhoA and β-actin genes and CD44 receptors in the cells, reveal that the network formation is synergistically influenced by the hydrogel stiffness and HA-Ph degradation. These findings highlight the potential of tailoring HA-based hydrogel properties to modulate human vascular endothelial cell responses, which is critical for advancing their application in vascular tissue engineering.
Publisher
John Wiley and Sons Inc
Source Title
Advanced Healthcare Materials
Volume (Issue)
13(17)
Page
2303787
Date of Issued
2024-07-01
Language
English
Handle URL
https://hdl.handle.net/11094/97143
PISSN
21922640
eISSN
21922659
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1002/adhm.202303787
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article