Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells

Li, Aonan; Sasaki, Jun-Ichi; Huang, Hailing; Abe, Gabriela L.; Inubushi, Toshihiro; Takahashi, Yusuke; Hayashi, Mikako; Imazato, Satoshi

doi:https://doi.org/10.1016/j.joen.2024.04.015

Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells

Li, Aonan; Sasaki, Jun-Ichi; Huang, Hailing et al.

Journal of Endodontics, 2024, 50(8), 1108-1116

Number of Access:1,375（2025-10-24 11:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97165

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Title	Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells
Creator	CreatorName Li, Aonan
	NRID 1000050530490 1000050530490 researchmap permalink Sasaki_JI Sasaki_JI Scopus Author ID 36138008500 36138008500 ORCID 0000-0002-0330-0397 0000-0002-0330-0397 CreatorName Sasaki, Jun-Ichi
	CreatorName Huang, Hailing
	NRID 1000030964952 1000030964952 ORCID 0000-0001-8843-1697 0000-0001-8843-1697 researchmap permalink gabriela_abe gabriela_abe CreatorName Abe, Gabriela L.
	NRID 1000030550941 1000030550941 researchmap permalink opathone0141 opathone0141 Scopus Author ID 23097933700 23097933700 ORCID 0000-0001-5303-4763 0000-0001-5303-4763 CreatorName Inubushi, Toshihiro
	NRID 1000060397693 1000060397693 ORCID 0000-0002-7974-1252 0000-0002-7974-1252 researchmap permalink read0210127 read0210127 Scopus Author ID 7408219812 7408219812 CreatorName Takahashi, Yusuke
	NRID 1000040271027 1000040271027 researchmap permalink read0185244 read0185244 Scopus Author ID 26021225700 26021225700 CreatorName Hayashi, Mikako
	NRID 1000080243244 1000080243244 ORCID 0000-0003-3655-4809 0000-0003-3655-4809 researchmap permalink read0014210 read0014210 Scopus Author ID 55994423600 55994423600 CreatorName Imazato, Satoshi
Subject	Dental pulp stem cells
	dentinogenesis
	heparan sulfate
	pulp capping
	vasculogenesis
Description	Li A., Sasaki J.I., Huang H., et al. Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells. Journal of Endodontics , (2024); https://doi.org/10.1016/j.joen.2024.04.015.
Abstract	Introduction: Heparan sulfate (HS) is a major component of dental pulp tissue. We previously reported that inhibiting HS biosynthesis impedes endothelial differentiation of dental pulp stem cells (DPSCs). However, the underlying mechanisms by which exogenous HS induces DPSC differentiation and pulp tissue regeneration remain unknown. This study explores the impact of exogenous HS on vasculogenesis and dentinogenesis of DPSCs both in vitro and in vivo. Methods: Human-derived DPSCs were cultured in endothelial and odontogenic differentiation media and treated with HS. Endothelial differentiation of DPSCs was investigated by real-time polymerase chain reaction and capillary sprouting assay. Odontogenic differentiation was assessed through real-time polymerase chain reaction and detection of mineralized dentin-like deposition. Additionally, the influence of HS on pulp tissue was assessed with a direct pulp capping model, in which HS was delivered to exposed pulp tissue in rats. Gelatin sponges were loaded with either phosphate-buffered saline or 101–102 μg/mL HS and placed onto the pulp tissue. Following a 28-day period, tissues were investigated by histological analysis and micro–computed tomography imaging. Results: HS treatment markedly increased expression levels of key endothelial and odontogenic genes, enhanced the formation of capillary-like structures, and promoted the deposition of mineralized matrices. Treatment of exposed pulp tissue with HS in the in vivo pulp capping study induced formation of capillaries and reparative dentin. Conclusions: Exogenous HS effectively promoted vasculogenesis and dentinogenesis of DPSCs in vitro and induced reparative dentin formation in vivo, highlighting its therapeutic potential for pulp capping treatment.
Publisher	Elsevier Inc.
Source Title	Journal of Endodontics
Volume (Issue)	50(8)
Page	1108-1116
Date of Issued	2024-05-07
Language	English
Handle URL	https://hdl.handle.net/11094/97165
PISSN	00992399
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.joen.2024.04.015
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Journal of Endodontics. 2024, 50(8), p. 1108-1116
DC.title	Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells
DC.creator	Li, Aonan
	Sasaki, Jun-Ichi
	Huang, Hailing
	Abe, Gabriela L.
	Inubushi, Toshihiro
	Takahashi, Yusuke
	Hayashi, Mikako
	Imazato, Satoshi
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-05-07
DC.identifier	https://doi.org/10.1016/j.joen.2024.04.015
DC.subject	Dental pulp stem cells
	dentinogenesis
	heparan sulfate
	pulp capping
	vasculogenesis
DC.description	Li A., Sasaki J.I., Huang H., et al. Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells. Journal of Endodontics , (2024); https://doi.org/10.1016/j.joen.2024.04.015.
DCTERMS.abstract	Introduction: Heparan sulfate (HS) is a major component of dental pulp tissue. We previously reported that inhibiting HS biosynthesis impedes endothelial differentiation of dental pulp stem cells (DPSCs). However, the underlying mechanisms by which exogenous HS induces DPSC differentiation and pulp tissue regeneration remain unknown. This study explores the impact of exogenous HS on vasculogenesis and dentinogenesis of DPSCs both in vitro and in vivo. Methods: Human-derived DPSCs were cultured in endothelial and odontogenic differentiation media and treated with HS. Endothelial differentiation of DPSCs was investigated by real-time polymerase chain reaction and capillary sprouting assay. Odontogenic differentiation was assessed through real-time polymerase chain reaction and detection of mineralized dentin-like deposition. Additionally, the influence of HS on pulp tissue was assessed with a direct pulp capping model, in which HS was delivered to exposed pulp tissue in rats. Gelatin sponges were loaded with either phosphate-buffered saline or 101–102 μg/mL HS and placed onto the pulp tissue. Following a 28-day period, tissues were investigated by histological analysis and micro–computed tomography imaging. Results: HS treatment markedly increased expression levels of key endothelial and odontogenic genes, enhanced the formation of capillary-like structures, and promoted the deposition of mineralized matrices. Treatment of exposed pulp tissue with HS in the in vivo pulp capping study induced formation of capillaries and reparative dentin. Conclusions: Exogenous HS effectively promoted vasculogenesis and dentinogenesis of DPSCs in vitro and induced reparative dentin formation in vivo, highlighting its therapeutic potential for pulp capping treatment.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	97165
citation_title	Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells
citation_author	Li, Aonan
	Sasaki, Jun-Ichi
	Huang, Hailing
	Abe, Gabriela L.
	Inubushi, Toshihiro
	Takahashi, Yusuke
	Hayashi, Mikako
	Imazato, Satoshi
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2024-05-07
citation_journal_title	Journal of Endodontics
citation_volume	50
citation_issue	8
citation_firstpage	1108
citation_lastpage	1116
citation_doi	https://doi.org/10.1016/j.joen.2024.04.015
citation_public_url	https://hdl.handle.net/11094/97165

Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells

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