Asporin and CD109, expressed in the injured neonatal spinal cord, attenuate axonal re-growth in vitro

Hosen, Sakura; Ikeda-Yorifuji, Iyo; Yamashita, Toshihide

Neuroscience Letters, 2024, 833, 137832

Number of Access:446（2025-08-26 02:04 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97175

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Title
Asporin and CD109, expressed in the injured neonatal spinal cord, attenuate axonal re-growth in vitro
Creator
CreatorName
Hosen, Sakura
NRID
1000030963342 1000030963342
researchmap permalink
iyoyorifuji iyoyorifuji
CreatorName
Ikeda-Yorifuji, Iyo
NRID
1000010301269 1000010301269
ORCID
0000-0003-4559-7018 0000-0003-4559-7018
researchmap permalink
ToshihideYamashita ToshihideYamashita
Scopus Author ID
35479670200 35479670200
CreatorName
Yamashita, Toshihide
Subject
Asporin: Cd109
Axonal regeneration
snRNA-seq
Spinal cord injury
TGF-β1
Description
Hosen S., Ikeda-Yorifuji I., Yamashita T.. Asporin and CD109, expressed in the injured neonatal spinal cord, attenuate axonal re-growth in vitro. Neuroscience Letters 833, 137832 (2024); https://doi.org/10.1016/j.neulet.2024.137832.
Abstract
Axonal regeneration is restricted in adults and causes irreversible motor dysfunction following spinal cord injury (SCI). In contrast, neonates have prominent regenerative potential and can restore their neural function. Although the distinct cellular responses in neonates have been studied, how they contribute to neural recovery remains unclear. To assess whether the secreted molecules in neonatal SCI can enhance neural regeneration, we re-analyzed the previously performed single-nucleus RNA-seq (snRNA-seq) and focused on Asporin and Cd109, the highly expressed genes in the injured neonatal spinal cord. In the present study, we showed that both these molecules were expressed in the injured spinal cords of adults and neonates. We treated the cortical neurons with recombinant Asporin or CD109 to observe their direct effects on neurons in vitro. We demonstrated that these molecules enhance neurite outgrowth in neurons. However, these molecules did not enhance re-growth of severed axons. Our results suggest that Asporin and CD109 influence neurites at the lesion site, rather than promoting axon regeneration, to restore neural function in neonates after SCI.
Publisher
Elsevier Ireland Ltd
Source Title
Neuroscience Letters
Volume (Issue)
833
Page
137832
Date of Issued
2024-06-11
Language
English
Handle URL
https://hdl.handle.net/11094/97175
PISSN
03043940
eISSN
18727972
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.neulet.2024.137832
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article