Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

Mwaniki, Stephen; Sawant, Priyanka; Osemwenkhae, Osaretin P. et al.

Genes to Cells, 2024, 29(8), 650-666

Number of Access:694（2025-08-14 12:12 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97194

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Title
Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis
Creator
CreatorName
Mwaniki, Stephen
CreatorName
Sawant, Priyanka
CreatorName
Osemwenkhae, Osaretin P.
NRID
1000050893913 1000050893913
ORCID
0000-0002-4350-7229 0000-0002-4350-7229
researchmap permalink
yurikafujita yurikafujita
Scopus Author ID
57146062800 57146062800
CreatorName
Fujita, Yurika
NRID
1000030869061 1000030869061
researchmap permalink
msrito msrito
Scopus Author ID
35278000500 35278000500
CreatorName
Ito, Masaru
NRID
1000090546293 1000090546293
researchmap permalink
afurukohri afurukohri
Scopus Author ID
25228605000 25228605000
CreatorName
Furukohri, Asako
NRID
1000000252578 1000000252578
ORCID
0000-0003-4207-8247 0000-0003-4207-8247
researchmap permalink
akirashinohara13 akirashinohara13
Scopus Author ID
7102342573 7102342573
CreatorName
Shinohara, Akira
Subject
cleavage
Dmc1
Mei5-Sae3
meiotic recombination
Rad51
Description
This is the peer reviewed version of the following article: Mwaniki S., Sawant P., Osemwenkhae O.P., et al. Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis. Genes to Cells, (2024) which has been published in final form at https://doi.org/10.1111/gtc.13138. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Abstract
Interhomolog recombination in meiosis is mediated by the Dmc1 recombinase. The Mei5-Sae3 complex of Saccharomyces cerevisiae promotes Dmc1 assembly and functions with Dmc1 for homology-mediated repair of meiotic DNA double-strand breaks. How Mei5-Sae3 facilitates Dmc1 assembly remains poorly understood. In this study, we created and characterized several mei5 mutants featuring the amino acid substitutions of basic residues. We found that Arg97 of Mei5, conserved in its ortholog, SFR1 (complex with SWI5), RAD51 mediator, in humans and other organisms, is critical for complex formation with Sae3 for Dmc1 assembly. Moreover, the substitution of either Arg117 or Lys133 with Ala in Mei5 resulted in the production of a C-terminal truncated Mei5 protein during yeast meiosis. Notably, the shorter Mei5-R117A protein was observed in meiotic cells but not in mitotic cells when expressed, suggesting a unique regulation of Dmc1-mediated recombination by posttranslational processing of Mei5-Sae3.
Publisher
John Wiley and Sons Inc
Source Title
Genes to Cells
Volume (Issue)
29(8)
Page
650-666
Date of Issued
2024-06-25
Language
English
Handle URL
https://hdl.handle.net/11094/97194
PISSN
13569597
eISSN
13652443
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1111/gtc.13138
Access Rights
open access
CopyRight
© 2024 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article