Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis

Sampathkumar, Arivarasan; Zhong, Chen; Tang, Yuting et al.

Scientific Reports, 2024, 14, 9550

Number of Access:573（2025-08-14 00:41 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97217

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Title
Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis
Creator
CreatorName
Sampathkumar, Arivarasan
CreatorName
Zhong, Chen
CreatorName
Tang, Yuting
NRID
1000050893913 1000050893913
ORCID
0000-0002-4350-7229 0000-0002-4350-7229
researchmap permalink
yurikafujita yurikafujita
Scopus Author ID
57146062800 57146062800
CreatorName
Fujita, Yurika
NRID
1000030869061 1000030869061
researchmap permalink
msrito msrito
Scopus Author ID
35278000500 35278000500
CreatorName
Ito, Masaru
NRID
1000000252578 1000000252578
ORCID
0000-0003-4207-8247 0000-0003-4207-8247
researchmap permalink
akirashinohara13 akirashinohara13
Scopus Author ID
7102342573 7102342573
CreatorName
Shinohara, Akira
Subject
Auxin degron
Homologous recombination
Meiosis
Recombination checkpoint
Rfa1
RPA
Synapsis checkpoint
Description
Sampathkumar A., Zhong C., Tang Y., et al. Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis. Scientific Reports 14, 9550 (2024); https://doi.org/10.1038/s41598-024-60082-x.
Abstract
DNA double-strand breaks (DSBs) activate DNA damage responses (DDRs) in both mitotic and meiotic cells. A single-stranded DNA (ssDNA) binding protein, Replication protein-A (RPA) binds to the ssDNA formed at DSBs to activate ATR/Mec1 kinase for the response. Meiotic DSBs induce homologous recombination monitored by a meiotic DDR called the recombination checkpoint that blocks the pachytene exit in meiotic prophase I. In this study, we further characterized the essential role of RPA in the maintenance of the recombination checkpoint during Saccharomyces cerevisiae meiosis. The depletion of an RPA subunit, Rfa1, in a recombination-defective dmc1 mutant, fully alleviates the pachytene arrest with the persistent unrepaired DSBs. RPA depletion decreases the activity of a meiosis-specific CHK2 homolog, Mek1 kinase, which in turn activates the Ndt80 transcriptional regulator for pachytene exit. These support the idea that RPA is a sensor of ssDNAs for the activation of meiotic DDR. Rfa1 depletion also accelerates the prophase I delay in the zip1 mutant defective in both chromosome synapsis and the recombination, consistent with the notion that the accumulation of ssDNAs rather than defective synapsis triggers prophase I delay in the zip1 mutant.
Publisher
Nature Research
Source Title
Scientific Reports
Volume (Issue)
14
Page
9550
Date of Issued
2024-04-25
Language
English
Handle URL
https://hdl.handle.net/11094/97217
eISSN
20452322
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1038/s41598-024-60082-x
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article