Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag

Lu, Kai; Wazawa, Tetsuichi; Matsuda, Tomoki; Shcherbakova, Daria M.; Verkhusha, Vladislav V.; Nagai, Takeharu

doi:https://doi.org/10.1038/s42003-024-06169-7

Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag

Lu, Kai; Wazawa, Tetsuichi; Matsuda, Tomoki et al.

Communications Biology, 2024, 7, 473

Number of Access:209（2025-06-08 13:00 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97218

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Title	Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag
Creator	NRID 1000050647980 1000050647980 researchmap permalink ka1lu ka1lu CreatorName Lu, Kai
	NRID 1000080359851 1000080359851 researchmap permalink wazawa wazawa CreatorName Wazawa, Tetsuichi
	NRID 1000050419206 1000050419206 researchmap permalink tikimaz tikimaz Scopus Author ID 7404349300 7404349300 CreatorName Matsuda, Tomoki
	CreatorName Shcherbakova, Daria M.
	CreatorName Verkhusha, Vladislav V.
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 CreatorName Nagai, Takeharu
Description	Lu K., Wazawa T., Matsuda T., et al. Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag. Communications Biology 7, 473 (2024); https://doi.org/10.1038/s42003-024-06169-7.
Abstract	Bacterial phytochromes are attractive molecular templates for engineering fluorescent proteins (FPs) because their near-infrared (NIR) emission significantly extends the spectral coverage of GFP-like FPs. Existing phytochrome-based FPs covalently bind heme-derived tetrapyrrole chromophores and exhibit constitutive fluorescence. Here we introduce Rep-miRFP, an NIR imaging probe derived from bacterial phytochrome, which interacts non-covalently and reversibly with biliverdin chromophore. In Rep-miRFP, the photobleached non-covalent adduct can be replenished with fresh biliverdin, restoring fluorescence. By exploiting this chromophore renewal capability, we demonstrate NIR PAINT nanoscopy in mammalian cells using Rep-miRFP.
Publisher	Nature Research
Source Title	Communications Biology
Volume (Issue)	7
Page	473
Date of Issued	2024-04-18
Language	English
Handle URL	https://hdl.handle.net/11094/97218
eISSN	23993642
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s42003-024-06169-7
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Communications Biology. 2024, 7, p. 473
DC.title	Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag
DC.creator	Lu, Kai
	Wazawa, Tetsuichi
	Matsuda, Tomoki
	Shcherbakova, Daria M.
	Verkhusha, Vladislav V.
	Nagai, Takeharu
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-04-18
DC.identifier	https://doi.org/10.1038/s42003-024-06169-7
DC.description	Lu K., Wazawa T., Matsuda T., et al. Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag. Communications Biology 7, 473 (2024); https://doi.org/10.1038/s42003-024-06169-7.
DCTERMS.abstract	Bacterial phytochromes are attractive molecular templates for engineering fluorescent proteins (FPs) because their near-infrared (NIR) emission significantly extends the spectral coverage of GFP-like FPs. Existing phytochrome-based FPs covalently bind heme-derived tetrapyrrole chromophores and exhibit constitutive fluorescence. Here we introduce Rep-miRFP, an NIR imaging probe derived from bacterial phytochrome, which interacts non-covalently and reversibly with biliverdin chromophore. In Rep-miRFP, the photobleached non-covalent adduct can be replenished with fresh biliverdin, restoring fluorescence. By exploiting this chromophore renewal capability, we demonstrate NIR PAINT nanoscopy in mammalian cells using Rep-miRFP.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	97218
citation_title	Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag
citation_author	Lu, Kai
	Wazawa, Tetsuichi
	Matsuda, Tomoki
	Shcherbakova, Daria M.
	Verkhusha, Vladislav V.
	Nagai, Takeharu
citation_publisher	Nature Research
citation_language	English
citation_date	2024-04-18
citation_journal_title	Communications Biology
citation_volume	7
citation_firstpage	473
citation_doi	https://doi.org/10.1038/s42003-024-06169-7
citation_public_url	https://hdl.handle.net/11094/97218

Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag

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