Metabolomic characterization of monoclonal antibody-producing Chinese hamster lung (CHL)-YN cells in glucose-controlled serum-free fed-batch operation

Sukwattananipaat, Puriwat; Kuroda, Hirotaka; Yamano-Adachi, Noriko et al.

Biotechnology and Bioengineering, 2024, 121(9), 2848-2867

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Identifier to cite or link to this item: https://hdl.handle.net/11094/97616

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Title
Metabolomic characterization of monoclonal antibody-producing Chinese hamster lung (CHL)-YN cells in glucose-controlled serum-free fed-batch operation
Creator
CreatorName
Sukwattananipaat, Puriwat
CreatorName
Kuroda, Hirotaka
NRID
1000020582795 1000020582795
ORCID
0000-0002-2167-5379 0000-0002-2167-5379
researchmap permalink
kibou kibou
Scopus Author ID
15137739600 15137739600
CreatorName
Yamano-Adachi, Noriko
NRID
1000000252586 1000000252586
ORCID
0000-0003-0635-3862 0000-0003-0635-3862
researchmap permalink
omasa omasa
Scopus Author ID
7003926741 7003926741
CreatorName
Omasa, Takeshi
Subject
Chinese hamster lung
CHL-YN cells
CHO cells
fed-batch
glucose feeding development
metabolomics
Description
Sukwattananipaat P., Kuroda H., Yamano-Adachi N., et al. Metabolomic characterization of monoclonal antibody-producing Chinese hamster lung (CHL)-YN cells in glucose-controlled serum-free fed-batch operation. Biotechnology and Bioengineering, (2024); https://doi.org/10.1002/bit.28777.
Abstract
The fast-growing Chinese hamster lung (CHL)-YN cell line was recently developed for monoclonal antibody production. In this study, we applied a serum-free fed-batch cultivation process to immunoglobulin (Ig)G1-producing CHL-YN cells, which were then used to design a dynamic glucose supply system to stabilize the extracellular glucose concentration based on glucose consumption. Glucose consumption of the cultures rapidly oscillated following three phases of glutamine metabolism: consumption, production, and re-consumption. Use of the dynamic glucose supply prolonged the viability of the CHL-YN-IgG1 cell cultures and increased IgG1 production. Liquid chromatography with tandem mass spectrometry-based target metabolomics analysis of the extracellular metabolites during the first glutamine shift was conducted to search for depleted compounds. The results suggest that the levels of four amino acids, namely arginine, aspartate, methionine, and serine, were sharply decreased in CHL-YN cells during glutamine production. Supporting evidence from metabolic and gene expression analyses also suggest that CHL-YN cells acquired ornithine- and cystathionine-production abilities that differed from those in Chinese hamster ovary-K1 cells, potentially leading to proline and cysteine biosynthesis.
Publisher
John Wiley and Sons Inc
Source Title
Biotechnology and Bioengineering
Volume (Issue)
121(9)
Page
2848-2867
Date of Issued
2024-06-22
Language
English
Handle URL
https://hdl.handle.net/11094/97616
PISSN
00063592
eISSN
10970290
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1002/bit.28777
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
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Version of Record
Category
学術雑誌論文 Journal Article