Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis

Harada, Shotaro; Koyama, Yoshihisa; Yoshioka, Yoshichika; Inohara, Hidenori; Shimada, Shoichi

doi:https://doi.org/10.1016/j.bbrc.2024.150153

Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis

Harada, Shotaro; Koyama, Yoshihisa; Yoshioka, Yoshichika et al.

Biochemical and Biophysical Research Communications, 2024, 723, 150153

Number of Access:647（2025-08-16 03:18 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97648

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Title	Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis
Creator	NRID 1000010824740 1000010824740 CreatorName Harada, Shotaro
	NRID 1000040397667 1000040397667 researchmap permalink koyamayoshihisa koyamayoshihisa Scopus Author ID 49761644100 49761644100 CreatorName Koyama, Yoshihisa
	NRID 1000000174897 1000000174897 CreatorName Yoshioka, Yoshichika
	NRID 1000000273657 1000000273657 researchmap permalink read0046473 read0046473 Scopus Author ID 7004501837 7004501837 CreatorName Inohara, Hidenori
	NRID 1000020216063 1000020216063 researchmap permalink 556shimada 556shimada Scopus Author ID 35452177800 35452177800 ORCID 0000-0001-5369-1069 0000-0001-5369-1069 CreatorName Shimada, Shoichi
Subject	ex vivo MRI
	In vivo MRI
	inner ear
	Magnetic resonance imaging
	Reissner's membrane
Description	Harada S., Koyama Y., Yoshioka Y., et al. Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis. Biochemical and Biophysical Research Communications 723, 150153 (2024); https://doi.org/10.1016/j.bbrc.2024.150153.
Abstract	Although research on hearing loss, including the identification of causative genes, has become increasingly active, the pathogenic mechanism of hearing loss remains unclear. One of the reasons for this is that the structure of the inner ear of mice, which is commonly used as a genetically modified animal model, is too small and complex, making it difficult to accurately capture abnormalities and dynamic changes in vivo. Especially, Reissner's membrane is a very important structure that separates the perilymph and endolymph of the inner ear. This malformation or damage induces abnormalities in hearing and balance. Until now, imaging analyses, such as magnetic resonance imaging (MRI) and computed tomography, are performed to investigate the inner ear structure in vivo; however, it has been difficult to analyze the small inner ear structure of mice owing to resolution. Therefore, there is an urgent need to develop an image analysis method that can accurately capture the structure of the inner ear of mice including Reissner's membrane, both dynamically and statically. This study aimed to investigate whether it is possible to accurately capture the structure (e.g., Reissner's membrane) and abnormalities of the inner ear of mice using an 11.7 T MRI. By combining two types of MRI methods, in vivo and ex vivo, we succeeded for the first time in capturing the fine structure of the normal mouse inner ear, such as the Reissner's membrane, and inflammatory lesions of otitis media mouse models in detail and accurately. In the future, we believe that understanding the state of Reissner's membrane during living conditions will greatly contribute to the development of research on inner ear issues, such as hearing loss.
Publisher	Elsevier B.V.
Source Title	Biochemical and Biophysical Research Communications
Volume (Issue)	723
Page	150153
Date of Issued	2024-09-03
Language	English
Handle URL	https://hdl.handle.net/11094/97648
PISSN	0006291X
eISSN	10902104
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bbrc.2024.150153
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2024, 723, p. 150153
DC.title	Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis
DC.creator	Harada, Shotaro
	Koyama, Yoshihisa
	Yoshioka, Yoshichika
	Inohara, Hidenori
	Shimada, Shoichi
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-03
DC.identifier	https://doi.org/10.1016/j.bbrc.2024.150153
DC.subject	ex vivo MRI
	In vivo MRI
	inner ear
	Magnetic resonance imaging
	Reissner's membrane
DC.description	Harada S., Koyama Y., Yoshioka Y., et al. Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis. Biochemical and Biophysical Research Communications 723, 150153 (2024); https://doi.org/10.1016/j.bbrc.2024.150153.
DCTERMS.abstract	Although research on hearing loss, including the identification of causative genes, has become increasingly active, the pathogenic mechanism of hearing loss remains unclear. One of the reasons for this is that the structure of the inner ear of mice, which is commonly used as a genetically modified animal model, is too small and complex, making it difficult to accurately capture abnormalities and dynamic changes in vivo. Especially, Reissner's membrane is a very important structure that separates the perilymph and endolymph of the inner ear. This malformation or damage induces abnormalities in hearing and balance. Until now, imaging analyses, such as magnetic resonance imaging (MRI) and computed tomography, are performed to investigate the inner ear structure in vivo; however, it has been difficult to analyze the small inner ear structure of mice owing to resolution. Therefore, there is an urgent need to develop an image analysis method that can accurately capture the structure of the inner ear of mice including Reissner's membrane, both dynamically and statically. This study aimed to investigate whether it is possible to accurately capture the structure (e.g., Reissner's membrane) and abnormalities of the inner ear of mice using an 11.7 T MRI. By combining two types of MRI methods, in vivo and ex vivo, we succeeded for the first time in capturing the fine structure of the normal mouse inner ear, such as the Reissner's membrane, and inflammatory lesions of otitis media mouse models in detail and accurately. In the future, we believe that understanding the state of Reissner's membrane during living conditions will greatly contribute to the development of research on inner ear issues, such as hearing loss.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	97648
citation_title	Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis
citation_author	Harada, Shotaro
	Koyama, Yoshihisa
	Yoshioka, Yoshichika
	Inohara, Hidenori
	Shimada, Shoichi
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2024-09-03
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	723
citation_firstpage	150153
citation_doi	https://doi.org/10.1016/j.bbrc.2024.150153
citation_public_url	https://hdl.handle.net/11094/97648

Visualization of Reissner's membrane in the mouse inner ear using highly sensitive magnetic resonance imaging analysis

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