journal article

Journal Article

Nucleic Acids Research. 2024, gkae622

The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA

Oxford University Press (OUP)

English

2024-07-16

https://doi.org/10.1093/nar/gkae622

Taniguchi Ichiro, Hirose Tetsuro, Ohno Mutsuhito. The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA. Nucleic Acids Research 12, 505 (2024); https://doi.org/10.1093/nar/gkae622.

RNA helicases are involved in RNA metabolism in an ATP-dependent manner. Although many RNA helicases unwind the RNA structure and/or remove proteins from the RNA, some can load their interacting proteins onto RNAs. Here, we developed an in vitro strategy to identify the ATP-dependent factors involved in spliceosomal uridine-rich small nuclear RNA (U snRNA) export. We identified the RNA helicase UAP56/DDX39B, a component of the mRNA export complex named the transcription-export (TREX) complex, and its closely related RNA helicase URH49/DDX39A as the factors that stimulated RNA binding of PHAX, an adapter protein for U snRNA export. ALYREF, another TREX component, acted as a bridge between PHAX and UAP56/DDX39B. We also showed that UAP56/DDX39B and ALYREF participate in U snRNA export through a mechanism distinct from that of mRNA export. This study describes a novel aspect of the TREX components for U snRNP biogenesis and highlights the loading activity of RNA helicases.

This article is licensed under a Creative Commons Attribution 4.0 International License.

97838

The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA

Oxford University Press (OUP)

English

2024-07-16

Nucleic Acids Research

gkae622

https://doi.org/10.1093/nar/gkae622

https://hdl.handle.net/11094/97838