Time-course study of genetic changes in periodontal ligament regeneration after tooth replantation in a mouse model

Ohshima, Jun; Abe, Shotaro; Morita, Masayoshi et al.

Scientific Reports, 2024, 14(1), 15502

Number of Access:678（2025-10-24 11:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97839

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Title
Time-course study of genetic changes in periodontal ligament regeneration after tooth replantation in a mouse model
Creator
NRID
1000030755450 1000030755450
researchmap permalink
johshima johshima
Scopus Author ID
6701723797 6701723797
CreatorName
Ohshima, Jun
CreatorName
Abe, Shotaro
CreatorName
Morita, Masayoshi
CreatorName
Tanaka, Nobutake
NRID
1000000714536 1000000714536
ORCID
0000-0001-6218-7112 0000-0001-6218-7112
researchmap permalink
MasayaYamaguchi MasayaYamaguchi
Scopus Author ID
16074710900 16074710900
CreatorName
Yamaguchi, Masaya
NRID
1000040271027 1000040271027
researchmap permalink
read0185244 read0185244
Scopus Author ID
26021225700 26021225700
CreatorName
Hayashi, Mikako
Description
Ohshima J., Abe S., Morita M., et al. Time-course study of genetic changes in periodontal ligament regeneration after tooth replantation in a mouse model. Scientific Reports 14, 15502 (2024); https://doi.org/10.1038/s41598-024-66542-8.
Abstract
This research focused on analyzing gene expression changes in the periodontal ligament (PDL) after tooth re-plantation to identify key genes and pathways involved in healing and regeneration. Utilizing a mouse model, mRNA was extracted from the PDL at various intervals post-replantation for RNA sequencing analysis, spanning from 3 to 56 days. The results revealed significant shifts in gene expression, particularly notable on day 28, supported by hierarchical clustering and principal component analysis. Gene ontology (GO) enrichment analysis highlighted an upregulation in olfactory receptor and G protein-coupled receptor signaling pathways at this time point. These findings were validated through reverse transcription-quantitative PCR (RT-qPCR), with immunochemical staining localizing olfactory receptor gene expression to the PDL and surrounding tissues. Moreover, a scratch assay indicated that olfactory receptor genes might facilitate wound healing in human PDL fibroblasts. These results underscore the importance of the 28-day post-transplant phase as a potential “tipping point” in PDL healing and regeneration. In conclusion, this research sheds light on the potential role of olfactory receptor genes in PDL regeneration, providing a foundation for developing new therapeutic approaches in tooth replantation and transplantation, with broader implications for regenerative medicine in oral health.
Publisher
Nature Research
Source Title
Scientific Reports
Volume (Issue)
14(1)
Page
15502
Date of Issued
2024-07-05
Language
English
Handle URL
https://hdl.handle.net/11094/97839
eISSN
20452322
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1038/s41598-024-66542-8
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
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Category
学術雑誌論文 Journal Article