Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging

Rui, Xuehui; Okamoto, Yukihiro; Fukushima, Shuichiro et al.

Scientific Reports, 2024, 14(1), 15831

Number of Access:61（2025-05-01 13:42 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97844

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Title
Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging
Creator
CreatorName
Rui, Xuehui
NRID
1000050503918 1000050503918
researchmap permalink
read1-okamoto read1-okamoto
Scopus Author ID
35269706600 35269706600
CreatorName
Okamoto, Yukihiro
NRID
1000040362644 1000040362644
researchmap permalink
read0107422 read0107422
Scopus Author ID
7202543214 7202543214
CreatorName
Fukushima, Shuichiro
NRID
1000040892683 1000040892683
ORCID
0000-0002-3461-3660 0000-0002-3461-3660
researchmap permalink
nozomiwatanabe nozomiwatanabe
Scopus Author ID
57190045741 57190045741
CreatorName
Morishita Watanabe, Nozomi
NRID
1000020311772 1000020311772
researchmap permalink
read0191167 read0191167
Scopus Author ID
6701925853 6701925853
CreatorName
Umakoshi, Hiroshi
Description
Rui X., Okamoto Y., Fukushima S., et al. Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging. Scientific Reports 14, 15831 (2024); https://doi.org/10.1038/s41598-024-65812-9.
Abstract
2-Hydroxyoleic acid (2-OHOA) has gained attention as a membrane lipid therapy (MLT) anti-cancer drug. However, in the viewpoint of anti-cancer drug, 2-OHOA shows poor water solubility and its effectiveness still has space for improvement. Thus, this study aimed to overcome the problems by formulating 2-OHOA into liposome dosage form. Furthermore, in the context of MLT reagents, the influence of 2-OHOA on the biophysical properties of the cytoplasmic membrane remains largely unexplored. To bridge this gap, our study specifically focused the alterations in cancer cell membrane fluidity and lipid packing characteristics before and after treatment. By using a two-photon microscope and the Laurdan fluorescence probe, we noted that liposomes incorporating 2-OHOA induced a more significant reduction in cancer cell membrane fluidity, accompanied by a heightened rate of cellular apoptosis when compared to the non-formulated 2-OHOA. Importantly, the enhanced efficacy of 2-OHOA within the liposomal formulation demonstrated a correlation with its endocytic uptake mechanism. In conclusion, our findings underscore the significant influence of 2-OHOA on the biophysical properties of cancer plasma membranes, emphasizing the potential of liposomes as an optimized delivery system for 2-OHOA in anti-cancer therapy.
Publisher
Nature Research
Source Title
Scientific Reports
Volume (Issue)
14(1)
Page
15831
Date of Issued
2024-07-09
Language
English
Handle URL
https://hdl.handle.net/11094/97844
eISSN
20452322
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1038/s41598-024-65812-9
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
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Category
学術雑誌論文 Journal Article