CRISPR screens identify genes essential for in vivo virulence among proteins of hyperLOPIT-unassigned subcellular localization in Toxoplasma

Tachibana, Yuta; Sasai, Miwa; Yamamoto, Masahiro

mBio, 2024, 15(9), e01728-24

Number of Access:404（2025-08-18 14:07 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97845

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Title
CRISPR screens identify genes essential for in vivo virulence among proteins of hyperLOPIT-unassigned subcellular localization in Toxoplasma
Creator
CreatorName
Tachibana, Yuta
NRID
1000030631551 1000030631551
ORCID
0000-0001-6440-3958 0000-0001-6440-3958
researchmap permalink
miwa-s miwa-s
Scopus Author ID
16319941500 16319941500
CreatorName
Sasai, Miwa
NRID
1000000444521 1000000444521
researchmap permalink
read0151116 read0151116
Scopus Author ID
55552856200 55552856200
CreatorName
Yamamoto, Masahiro
Subject
Toxoplasma
CRISPR screen
hyperLOPIT-unassigned virulence factors
Description
Tachibana Yuta, Sasai Miwa, Yamamoto Masahiro, et al. CRISPR screens identify genes essential for in vivo virulence among proteins of hyperLOPIT-unassigned subcellular localization in Toxoplasma. mBio , e01728-24 (2024); https://doi.org/10.1128/mbio.01728-24.
Abstract
The research field to identify and characterize genes essential for in vivo virulence in Toxoplasma gondii has been dramatically advanced by a series of in vivo clustered regularly interspaced short palindromic repeats (CRISPR) screens. Although subcellular localizations of thousands of proteins were predicted by the spatial proteomic method called hyperLOPIT, those of more than 1,000 proteins remained unassigned, and their essentiality in virulence was also unknown. In this study, we generated two small-scale gRNA libraries targeting approximately 600 hyperLOPIT-unassigned proteins and performed in vivo CRISPR screens. As a result, we identified several genes essential for in vivo virulence that were previously unreported. We further characterized two candidates, TgGTPase and TgRimM, which are localized in the cytoplasm and the apicoplast, respectively. Both genes are essential for parasite virulence and widely conserved in the phylum Apicomplexa. Collectively, our current study provides a resource for estimating the in vivo essentiality of Toxoplasma proteins with previously unknown localizations.
Publisher
American Society for Microbiology
Source Title
mBio
Volume (Issue)
15(9)
Page
e01728-24
Date of Issued
2024-07-31
Language
English
Handle URL
https://hdl.handle.net/11094/97845
eISSN
21507511
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1128/mbio.01728-24
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article