Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues

Watanabe, Kenichi; Takayama, Sayo; Yamada, Toichiro; Hashimoto, Masayo; Tadano, Jun; Nakagawa, Tetsuya; Watanabe, Takao; Fukusaki, Eiichiro; Miyawaki, Izuru; Shimma, Shuichi

doi:https://doi.org/10.1007/s00216-024-05477-5

Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues

Watanabe, Kenichi; Takayama, Sayo; Yamada, Toichiro et al.

Analytical and Bioanalytical Chemistry, 2024, 416(26), 5579-5593

Number of Access:894（2025-08-13 23:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98111

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Title	Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
Creator	CreatorName Watanabe, Kenichi
	CreatorName Takayama, Sayo
	CreatorName Yamada, Toichiro
	CreatorName Hashimoto, Masayo
	CreatorName Tadano, Jun
	CreatorName Nakagawa, Tetsuya
	CreatorName Watanabe, Takao
	NRID 1000040273594 1000040273594 ORCID 0000-0003-3069-5547 0000-0003-3069-5547 researchmap permalink fukusaki-eiichiro fukusaki-eiichiro Scopus Author ID 35473325000 35473325000 CreatorName Fukusaki, Eiichiro
	CreatorName Miyawaki, Izuru
	NRID 1000030515896 1000030515896 researchmap permalink shimmax shimmax Scopus Author ID 23486936900 23486936900 CreatorName Shimma, Shuichi
Subject	Brain
	Clozapine
	Matrix-assisted laser desorption/ionization
	Neurotransmitters
	Quantitative mass spectrometry imaging
Description	The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05477-5.
Abstract	Understanding the relationship between the concentration of a drug and its therapeutic efficacy or side effects is crucial in drug development, especially to understand therapeutic efficacy in central nervous system drug, quantifying drug-induced site-specific changes in the levels of endogenous metabolites, such as neurotransmitters. In recent times, evaluation of quantitative distribution of drugs and endogenous metabolites using matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) has attracted much attention in drug discovery research. However, MALDI-MSI quantification (quantitative mass spectrometry imaging, QMSI) is an emerging technique, and needs to be further developed for practicable and convenient use in drug discovery research. In this study, we developed a reliable QMSI method for quantification of clozapine (antipsychotic drug) and dopamine and its metabolites in the rat brain using MALDI-MSI. An improved mimetic tissue model using powdered frozen tissue for QMSI was established as an alternative method, enabling the accurate quantification of clozapine levels in the rat brain. Furthermore, we used the improved method to evaluate drug-induced fluctuations in the concentrations of dopamine and its metabolites. This method can quantitatively evaluate drug localization in the brain and drug-induced changes in the concentration of endogenous metabolites, demonstrating the usefulness of QMSI.
Publisher	Springer Science and Business Media Deutschland GmbH
Source Title	Analytical and Bioanalytical Chemistry
Volume (Issue)	416(26)
Page	5579-5593
Date of Issued	2024-08-10
Language	English
Handle URL	https://hdl.handle.net/11094/98111
PISSN	16182642
eISSN	16182650
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1007/s00216-024-05477-5
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Analytical and Bioanalytical Chemistry. 2024, 416(26), p. 5579-5593
DC.title	Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
DC.creator	Watanabe, Kenichi
	Takayama, Sayo
	Yamada, Toichiro
	Hashimoto, Masayo
	Tadano, Jun
	Nakagawa, Tetsuya
	Watanabe, Takao
	Fukusaki, Eiichiro
	Miyawaki, Izuru
	Shimma, Shuichi
DC.publisher	Springer Science and Business Media Deutschland GmbH
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-08-10
DC.identifier	https://doi.org/10.1007/s00216-024-05477-5
DC.subject	Brain
	Clozapine
	Matrix-assisted laser desorption/ionization
	Neurotransmitters
	Quantitative mass spectrometry imaging
DC.description	The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05477-5.
DCTERMS.abstract	Understanding the relationship between the concentration of a drug and its therapeutic efficacy or side effects is crucial in drug development, especially to understand therapeutic efficacy in central nervous system drug, quantifying drug-induced site-specific changes in the levels of endogenous metabolites, such as neurotransmitters. In recent times, evaluation of quantitative distribution of drugs and endogenous metabolites using matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) has attracted much attention in drug discovery research. However, MALDI-MSI quantification (quantitative mass spectrometry imaging, QMSI) is an emerging technique, and needs to be further developed for practicable and convenient use in drug discovery research. In this study, we developed a reliable QMSI method for quantification of clozapine (antipsychotic drug) and dopamine and its metabolites in the rat brain using MALDI-MSI. An improved mimetic tissue model using powdered frozen tissue for QMSI was established as an alternative method, enabling the accurate quantification of clozapine levels in the rat brain. Furthermore, we used the improved method to evaluate drug-induced fluctuations in the concentrations of dopamine and its metabolites. This method can quantitatively evaluate drug localization in the brain and drug-induced changes in the concentration of endogenous metabolites, demonstrating the usefulness of QMSI.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98111
citation_title	Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
citation_author	Watanabe, Kenichi
	Takayama, Sayo
	Yamada, Toichiro
	Hashimoto, Masayo
	Tadano, Jun
	Nakagawa, Tetsuya
	Watanabe, Takao
	Fukusaki, Eiichiro
	Miyawaki, Izuru
	Shimma, Shuichi
citation_publisher	Springer Science and Business Media Deutschland GmbH
citation_language	English
citation_date	2024-08-10
citation_journal_title	Analytical and Bioanalytical Chemistry
citation_volume	416
citation_issue	26
citation_firstpage	5579
citation_lastpage	5593
citation_doi	https://doi.org/10.1007/s00216-024-05477-5
citation_public_url	https://hdl.handle.net/11094/98111

Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues

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