Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers

Sasaki, Naoko; Asano, Yoshiya; Sorayama, Yukiko; Kamimura, Chihiro; Kitano, Shiro; Irie, Shinji; Katayama, Ryohei; Shimoda, Hiroshi; Matsusaki, Michiya

doi:https://doi.org/10.1016/j.actbio.2024.07.001

Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers

Sasaki, Naoko; Asano, Yoshiya; Sorayama, Yukiko et al.

Acta Biomaterialia, 2024, 185, 161-172

Number of Access:794（2025-10-31 17:25 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98163

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Title	Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers
Creator	NRID 1000010388762 1000010388762 researchmap permalink sasaki_n sasaki_n CreatorName Sasaki, Naoko
	NRID 1000050359494 1000050359494 CreatorName Asano, Yoshiya
	CreatorName Sorayama, Yukiko
	CreatorName Kamimura, Chihiro
	CreatorName Kitano, Shiro
	CreatorName Irie, Shinji
	NRID 1000060435542 1000060435542 CreatorName Katayama, Ryohei
	NRID 1000020274748 1000020274748 CreatorName Shimoda, Hiroshi
	NRID 1000000419467 1000000419467 researchmap permalink read0093431 read0093431 Scopus Author ID 6701561701 6701561701 ORCID 0000-0003-4294-9313 0000-0003-4294-9313 CreatorName Matsusaki, Michiya
Subject	3D cancer-stromal tissue
	Cancer stem cell marker
	Collagen microfiber (CMF)
	Stiffness
Description	Sasaki N., Asano Y., Sorayama Y., et al. Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers. Acta Biomaterialia 185, 161 (2024); https://doi.org/10.1016/j.actbio.2024.07.001.
Abstract	The extracellular matrix (ECM) of cancer tissues is rich in dense collagen, contributing to the stiffening of these tissues. Increased stiffness has been reported to promote cancer cell proliferation, invasion, metastasis, and prevent drug delivery. Replicating the structure and mechanical properties of cancer tissue in vitro is essential for developing cancer treatment drugs that target these properties. In this study, we recreated specific characteristics of cancer tissue, such as collagen density and high elastic modulus, using a colorectal cancer cell line as a model. Using our original material, collagen microfibers (CMFs), and a constructed three-dimensional (3D) cancer-stromal tissue model, we successfully reproduced an ECM highly similar to in vivo conditions. Furthermore, our research demonstrated that cancer stem cell markers expressed in the 3D cancer-stromal tissue model more closely mimic in vivo conditions than traditional two-dimensional cell cultures. We also found that CMFs might affect an impact on how cancer cells express these markers. Our 3D CMF-based model holds promise for enhancing our understanding of colorectal cancer and advancing therapeutic approaches. Statement of significance: Reproducing the collagen content and stiffness of cancer tissue is crucial in comprehending the properties of cancer and advancing anticancer drug development. Nonetheless, the use of collagen as a scaffold material has posed challenges due to its poor solubility, hindering the replication of a cancer microenvironment. In this study, we have successfully recreated cancer tissue-specific characteristics such as collagen density, stiffness, and the expression of cancer stem cell markers in three-dimensional (3D) colorectal cancer stromal tissue, utilizing a proprietary material known as collagen microfiber (CMF). CMF proves to be an ideal scaffold material for replicating cancer stromal tissue, and these 3D tissues constructed with CMFs hold promise in contributing to our understanding of cancer and the development of therapeutic drugs.
Publisher	Elsevier Ltd
Source Title	Acta Biomaterialia
Volume (Issue)	185
Page	161-172
Date of Issued	2024-09-01
Language	English
Handle URL	https://hdl.handle.net/11094/98163
PISSN	17427061
eISSN	18787568
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.actbio.2024.07.001
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Acta Biomaterialia. 2024, 185, p. 161-172
DC.title	Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers
DC.creator	Sasaki, Naoko
	Asano, Yoshiya
	Sorayama, Yukiko
	Kamimura, Chihiro
	Kitano, Shiro
	Irie, Shinji
	Katayama, Ryohei
	Shimoda, Hiroshi
	Matsusaki, Michiya
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-01
DC.identifier	https://doi.org/10.1016/j.actbio.2024.07.001
DC.subject	3D cancer-stromal tissue
	Cancer stem cell marker
	Collagen microfiber (CMF)
	Stiffness
DC.description	Sasaki N., Asano Y., Sorayama Y., et al. Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers. Acta Biomaterialia 185, 161 (2024); https://doi.org/10.1016/j.actbio.2024.07.001.
DCTERMS.abstract	The extracellular matrix (ECM) of cancer tissues is rich in dense collagen, contributing to the stiffening of these tissues. Increased stiffness has been reported to promote cancer cell proliferation, invasion, metastasis, and prevent drug delivery. Replicating the structure and mechanical properties of cancer tissue in vitro is essential for developing cancer treatment drugs that target these properties. In this study, we recreated specific characteristics of cancer tissue, such as collagen density and high elastic modulus, using a colorectal cancer cell line as a model. Using our original material, collagen microfibers (CMFs), and a constructed three-dimensional (3D) cancer-stromal tissue model, we successfully reproduced an ECM highly similar to in vivo conditions. Furthermore, our research demonstrated that cancer stem cell markers expressed in the 3D cancer-stromal tissue model more closely mimic in vivo conditions than traditional two-dimensional cell cultures. We also found that CMFs might affect an impact on how cancer cells express these markers. Our 3D CMF-based model holds promise for enhancing our understanding of colorectal cancer and advancing therapeutic approaches. Statement of significance: Reproducing the collagen content and stiffness of cancer tissue is crucial in comprehending the properties of cancer and advancing anticancer drug development. Nonetheless, the use of collagen as a scaffold material has posed challenges due to its poor solubility, hindering the replication of a cancer microenvironment. In this study, we have successfully recreated cancer tissue-specific characteristics such as collagen density, stiffness, and the expression of cancer stem cell markers in three-dimensional (3D) colorectal cancer stromal tissue, utilizing a proprietary material known as collagen microfiber (CMF). CMF proves to be an ideal scaffold material for replicating cancer stromal tissue, and these 3D tissues constructed with CMFs hold promise in contributing to our understanding of cancer and the development of therapeutic drugs.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98163
citation_title	Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers
citation_author	Sasaki, Naoko
	Asano, Yoshiya
	Sorayama, Yukiko
	Kamimura, Chihiro
	Kitano, Shiro
	Irie, Shinji
	Katayama, Ryohei
	Shimoda, Hiroshi
	Matsusaki, Michiya
citation_publisher	Elsevier Ltd
citation_language	English
citation_date	2024-09-01
citation_journal_title	Acta Biomaterialia
citation_volume	185
citation_firstpage	161
citation_lastpage	172
citation_doi	https://doi.org/10.1016/j.actbio.2024.07.001
citation_public_url	https://hdl.handle.net/11094/98163

Promoting biological similarity by collagen microfibers in 3D colorectal cancer-stromal tissue: Replicating mechanical properties and cancer stem cell markers

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