Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells

Kobayashi, Jundai; Higashisaka, Kazuma; Muranaka, Mizuki; Xie, Yankun; Okuno, Wakako; Haga, Yuya; Tsutsumi, Yasuo

doi:https://doi.org/10.1016/j.bbrc.2024.150488

Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells

Kobayashi, Jundai; Higashisaka, Kazuma; Muranaka, Mizuki et al.

Biochemical and Biophysical Research Communications, 2024, 736, 150488

Number of Access:676（2025-07-23 00:52 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98176

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Title	Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells
Creator	CreatorName Kobayashi, Jundai
	NRID 1000020646757 1000020646757 researchmap permalink read646757 read646757 Scopus Author ID 35603386200 35603386200 CreatorName Higashisaka, Kazuma
	CreatorName Muranaka, Mizuki
	CreatorName Xie, Yankun
	CreatorName Okuno, Wakako
	NRID 1000030888863 1000030888863 ORCID 0000-0003-4148-2256 0000-0003-4148-2256 researchmap permalink 30888863 30888863 Scopus Author ID 57211023415 57211023415 CreatorName Haga, Yuya
	NRID 1000050263306 1000050263306 researchmap permalink ytsutsumi ytsutsumi Scopus Author ID 7202474093 7202474093 CreatorName Tsutsumi, Yasuo
Subject	Lysosome
	Placental toxicity
	Silica nanoparticle
	Trophoblast
Description	Kobayashi J., Higashisaka K., Muranaka M., et al. Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells. Biochemical and Biophysical Research Communications 736, 150488 (2024); https://doi.org/10.1016/j.bbrc.2024.150488.
Abstract	Nanoparticles have useful functions due to the characteristics conferred on them by an increase in their specific surface area, and they have already been put into practical use in products in various industrial fields. Although exposure to nanoparticles in daily life is unavoidable for pregnant women, studies that evaluate the toxicity of nanoparticles in pregnant women are lacking. To redress this, we have focused on the placenta and have previously revealed that nanoparticles can show placental toxicity. However, there is still little knowledge regarding the behavior of nanoparticles within placental cells, which would enable us to understand their mode of action. Here, we tried to clarify the intracellular localization of silica nanoparticles in placental cells and how this affects placental toxicity. We analyzed the uptake of silica nanoparticles with a diameter of 10 nm (nSP10) into JEG-3 cells, a human choriocarcinoma cell line. Flow cytometry analysis showed that nSP10 labelled with red fluorescence were taken up into JEG-3 cells, and that pre-treatment with the endocytosis inhibitor cytochalasin D inhibited their uptake, suggesting that nSP10 are taken up into JEG-3 cells by the endocytic pathway. Moreover, confocal microscopy revealed that nSP10 are prominently localized in lysosomes. Staining with LysoTracker showed that nSP10 treatment increased the acidic compartment of JEG-3 cells, suggesting lysosome accumulation and swelling. These results indicate that nSP10 taken into placental cells are transferred to lysosomes and may cause lysosomal dysfunction.
Publisher	Elsevier B.V.
Source Title	Biochemical and Biophysical Research Communications
Volume (Issue)	736
Page	150488
Date of Issued	2024-12-03
Language	English
Handle URL	https://hdl.handle.net/11094/98176
PISSN	0006291X
eISSN	10902104
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bbrc.2024.150488
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2024, 736, p. 150488
DC.title	Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells
DC.creator	Kobayashi, Jundai
	Higashisaka, Kazuma
	Muranaka, Mizuki
	Xie, Yankun
	Okuno, Wakako
	Haga, Yuya
	Tsutsumi, Yasuo
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-12-03
DC.identifier	https://doi.org/10.1016/j.bbrc.2024.150488
DC.subject	Lysosome
	Placental toxicity
	Silica nanoparticle
	Trophoblast
DC.description	Kobayashi J., Higashisaka K., Muranaka M., et al. Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells. Biochemical and Biophysical Research Communications 736, 150488 (2024); https://doi.org/10.1016/j.bbrc.2024.150488.
DCTERMS.abstract	Nanoparticles have useful functions due to the characteristics conferred on them by an increase in their specific surface area, and they have already been put into practical use in products in various industrial fields. Although exposure to nanoparticles in daily life is unavoidable for pregnant women, studies that evaluate the toxicity of nanoparticles in pregnant women are lacking. To redress this, we have focused on the placenta and have previously revealed that nanoparticles can show placental toxicity. However, there is still little knowledge regarding the behavior of nanoparticles within placental cells, which would enable us to understand their mode of action. Here, we tried to clarify the intracellular localization of silica nanoparticles in placental cells and how this affects placental toxicity. We analyzed the uptake of silica nanoparticles with a diameter of 10 nm (nSP10) into JEG-3 cells, a human choriocarcinoma cell line. Flow cytometry analysis showed that nSP10 labelled with red fluorescence were taken up into JEG-3 cells, and that pre-treatment with the endocytosis inhibitor cytochalasin D inhibited their uptake, suggesting that nSP10 are taken up into JEG-3 cells by the endocytic pathway. Moreover, confocal microscopy revealed that nSP10 are prominently localized in lysosomes. Staining with LysoTracker showed that nSP10 treatment increased the acidic compartment of JEG-3 cells, suggesting lysosome accumulation and swelling. These results indicate that nSP10 taken into placental cells are transferred to lysosomes and may cause lysosomal dysfunction.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	98176
citation_title	Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells
citation_author	Kobayashi, Jundai
	Higashisaka, Kazuma
	Muranaka, Mizuki
	Xie, Yankun
	Okuno, Wakako
	Haga, Yuya
	Tsutsumi, Yasuo
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2024-12-03
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	736
citation_firstpage	150488
citation_doi	https://doi.org/10.1016/j.bbrc.2024.150488
citation_public_url	https://hdl.handle.net/11094/98176

Localization of silica nanoparticles to lysosome causes lysosomal dysfunction in JEG-3 cells

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