MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice

Wang, Haoting; Kobayashi, Hiroko; Shimada, Keisuke et al.

Journal of Cell Science, 2024, 137(16), jcs261962

Number of Access:902（2025-08-22 01:08 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98222

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
JCellSci_137_16_jcs261962 pdf None 172 KB   2025.08.29  

Item Information

Output File Export EndNote Basic Export Mendeley

Title
MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice
Creator
CreatorName
Wang, Haoting
CreatorName
Kobayashi, Hiroko
NRID
1000060779601 1000060779601
ORCID
0000-0003-3739-7163 0000-0003-3739-7163
researchmap permalink
nununununu nununununu
Scopus Author ID
56479808000 56479808000
CreatorName
Shimada, Keisuke
CreatorName
Oura, Seiya
ORCID
0000-0003-4579-5487 0000-0003-4579-5487
CreatorName
Oyama, Yuki
NRID
1000050981958 1000050981958
researchmap permalink
hiroakikitakaze hiroakikitakaze
CreatorName
Kitakaze, Hiroaki
NRID
1000050712551 1000050712551
CreatorName
Noda, Taichi
NRID
1000010343245 1000010343245
researchmap permalink
read0092303 read0092303
Scopus Author ID
6603956412 6603956412
CreatorName
Yabuta, Norikazu
NRID
1000050604732 1000050604732
ORCID
0000-0003-4758-5803 0000-0003-4758-5803
researchmap permalink
hmiyata hmiyata
Scopus Author ID
37161837000 37161837000
CreatorName
Miyata, Haruhiko
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
Central pair
Centriole
Flagellum formation
Genome editing
Knockout mice
Description
Wang H., Kobayashi H., Shimada K., et al. MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice. Journal of Cell Science 137, jcs261962 (2024); https://doi.org/10.1242/jcs.261962.
Abstract
The structure of the sperm flagellar axoneme is highly conserved across species and serves the essential function of generating motility to facilitate the meeting of spermatozoa with the egg. During spermiogenesis, the axoneme elongates from the centrosome, and subsequently the centrosome docks onto the nuclear envelope to continue tail biogenesis. Mycbpap is expressed predominantly in mouse and human testes and conserved in Chlamydomonas as FAP147. A previous cryo-electron microscopy analysis has revealed the localization of FAP147 to the central apparatus of the axoneme. Here, we generated Mycbpap-knockout mice and demonstrated the essential role of Mycbpap in male fertility. Deletion of Mycbpap led to disrupted centrosome–nuclear envelope docking and abnormal flagellar biogenesis. Furthermore, we generated transgenic mice with tagged MYCBPAP, which restored the fertility of Mycbpap-knockout males. Interactome analyses of MYCBPAP using Mycbpap transgenic mice unveiled binding partners of MYCBPAP including central apparatus proteins, such as CFAP65 and CFAP70, which constitute the C2a projection, and centrosome-associated proteins, such as CCP110. These findings provide insights into a MYCBPAP-dependent regulation of the centrosome–nuclear envelope docking and sperm tail biogenesis.
Publisher
Company of Biologists Ltd
Source Title
Journal of Cell Science
Volume (Issue)
137(16)
Page
jcs261962
Date of Issued
2024-08-29
Language
English
Handle URL
https://hdl.handle.net/11094/98222
PISSN
00219533
eISSN
14779137
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1242/jcs.261962
Access Rights
embargoed access
CopyRight
The full-text file will be made open to the public on 29 August 2025 in accordance with the publisher's policy.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article