Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department

Nagatomi, Hikaru; Sada, Ryuichi Minoda; Abe, Noriyuki et al.

Journal of Infection and Chemotherapy, 2024, 30(8), 768-772

Number of Access:372（2025-08-14 02:29 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98225

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Title
Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department
Creator
CreatorName
Nagatomi, Hikaru
NRID
1000040961230 1000040961230
ORCID
0000-0002-1455-6822 0000-0002-1455-6822
researchmap permalink
sadametal sadametal
Scopus Author ID
59319135000 59319135000
CreatorName
Sada, Ryuichi Minoda
CreatorName
Abe, Noriyuki
CreatorName
Miyake, Hirofumi
CreatorName
Akebo, Hiroyuki
Subject
Gram stain
Pyuria
Sternheimer stain
Description
Nagatomi H., Sada R.M., Abe N., et al. Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department. Journal of Infection and Chemotherapy 30, 768 (2024); https://doi.org/10.1016/j.jiac.2024.02.019.
Abstract
Introduction: Qualitative urinalysis using the Sternheimer stain is a common method in Japan for identifying bacteriuria, but there is a lack of studies examining its test characteristics. In this study, we aimed to investigate the sensitivity and specificity of the Sternheimer stain for urine culture results and compare it with the sensitivity and specificity of the Gram stain. Our goal was to determine the usefulness of the Sternheimer stain in identifying bacteriuria. Patients and methods: Among 986 patients aged 16 years or older from whom samples for both urinalysis and urine culture were obtained at the emergency room of Tenri Hospital from January 2019 to December 2019, 342 patients with pyuria, defined as the presence of 10 or more white cells per cubic millimeter in a urine specimen, who had not received prior antimicrobial therapy were included. Urine cultures were used for comparison to determine the sensitivity and specificity of Sternheimer and Gram stain in this patient group. A positive Sternheimer stain result was defined as bacteriuria ≥ (1+), and that of Gram stain was defined as ≥ 1/1 field of high-power ( × 1000) oil immersion. Results: Using urine culture results for comparison, the sensitivity of Sternheimer stain was 92.2%, the specificity was 48.5%, the positive likelihood ratio was 1.79, and the negative likelihood ratio was 0.16. Discussion: Sternheimer stain is a rapid and useful method to exclude bacteriuria in a group of patients with pyuria in the emergency department.
Publisher
Elsevier B.V.
Source Title
Journal of Infection and Chemotherapy
Volume (Issue)
30(8)
Page
768-772
Date of Issued
2024-08-01
Language
English
Handle URL
https://hdl.handle.net/11094/98225
PISSN
1341321X
eISSN
14377780
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1016/j.jiac.2024.02.019
Access Rights
embargoed access
CopyRight
© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
The full-text file will be made open to the public on 1 August 2025 in accordance with the publisher's policy.
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article