Synergistic effects of arginine and fluoride on human dental biofilm control

Kuriki, Nanako; Asahi, Yoko; Okamoto, Motoki et al.

Journal of Dentistry, 2024, 149, 105307

Number of Access:50（2025-05-12 12:37 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98327

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Title
Synergistic effects of arginine and fluoride on human dental biofilm control
Creator
NRID
1000060781432 1000060781432
researchmap permalink
nkuriki nkuriki
CreatorName
Kuriki, Nanako
NRID
1000050456943 1000050456943
researchmap permalink
yasahi yasahi
Scopus Author ID
7006117262 7006117262
CreatorName
Asahi, Yoko
NRID
1000060755354 1000060755354
CreatorName
Okamoto, Motoki
NRID
1000050218286 1000050218286
CreatorName
Noiri, Yuichiro
NRID
1000050116000 1000050116000
researchmap permalink
read0017458 read0017458
CreatorName
Ebisu, Shigeyuki
researchmap permalink
read0134202 read0134202
Scopus Author ID
6507889282 6507889282
CreatorName
Machi, Hiroyuki
NRID
1000070420049 1000070420049
CreatorName
Suzuki, Maiko
NRID
1000040271027 1000040271027
researchmap permalink
read0185244 read0185244
Scopus Author ID
26021225700 26021225700
CreatorName
Hayashi, Mikako
Subject
Arginine
Biofilms
Fluoride
In situ
Microbiome
Next-generation sequencing
Description
Kuriki N., Asahi Y., Okamoto M., et al. Synergistic effects of arginine and fluoride on human dental biofilm control. Journal of Dentistry 149, 105307 (2024); https://doi.org/10.1016/j.jdent.2024.105307.
Abstract
Objectives: The aim of this study was to quantitatively and comprehensively investigate the combined effects of arginine and fluoride on the suppression of pathogenicity using an in situ biofilm model and next-generation sequencing (NGS). Methods: Using the in situ model, dental biofilms were formed and the viable bacterial counts and arginine activity in the arginine- and fluoride-containing dentifrice and control groups were measured. We also compared their effects on the bacterial microbiota and predictive functional factors in the control, arginine (arg), and arginine + fluoride (argF) groups using NGS analysis. Results: Compared to the control treatment, the use of 8 % arginine and 1450 ppm fluoride toothpaste resulted in significantly high oral NH4+ concentrations without affecting the number of viable bacteria (P < 0.05). NGS analysis revealed that the oral microbiota of the control, arg, and argF groups were significantly different. Heat map analysis of the predicted functional factors revealed that the arg group had different properties from the other groups and activated specific substrate metabolic pathways; contrastingly, argF treatment inhibited the activity of these pathways and prevented an increase in the abundance of bacterial genera that utilize substrates such as sucrose, suggesting the synergistic effect of arginine and fluoride. Conclusions: This study indicates that the combination of arginine and fluoride has a synergistic effect on the bacterial microbiota and pathogenicity of dental biofilms compared with arginine alone. Clinical significance: Our findings suggest that the combination of arginine and fluoride could be used as an effective prebiotic and may inhibit the growth of bacteria associated with dental diseases.
Publisher
Elsevier Ltd
Source Title
Journal of Dentistry
Volume (Issue)
149
Page
105307
Date of Issued
2024-10-01
Language
English
Handle URL
https://hdl.handle.net/11094/98327
PISSN
03005712
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.jdent.2024.105307
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article