Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue

Li, Junjun; Li, Menglu; Nawa, Yasunori; Liu, Yuting; Bando, Kazuki; Hua, Ying; Sun, Lifu; Fujita, Satoshi; Sawa, Yoshiki; Fujita, Katsumasa; Liu, Li

doi:https://doi.org/10.1021/acs.analchem.4c03871

Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue

Li, Junjun; Li, Menglu; Nawa, Yasunori et al.

Analytical Chemistry, 2024, 96(39), 15765-15772

Number of Access:1,228（2025-08-27 17:02 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98346

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Title	Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue
Creator	NRID 1000010723786 1000010723786 ORCID 0000-0002-7473-4918 0000-0002-7473-4918 researchmap permalink liJJ liJJ Scopus Author ID 52464043600 52464043600 CreatorName Li, Junjun
	CreatorName Li, Menglu
	CreatorName Nawa, Yasunori
	CreatorName Liu, Yuting
	NRID 1000040795952 1000040795952 researchmap permalink kazukibando kazukibando Scopus Author ID 11540437400 11540437400 CreatorName Bando, Kazuki
	CreatorName Hua, Ying
	CreatorName Sun, Lifu
	CreatorName Fujita, Satoshi
	NRID 1000000243220 1000000243220 researchmap permalink y-sawa0703 y-sawa0703 Scopus Author ID 55428162700 55428162700 CreatorName Sawa, Yoshiki
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 CreatorName Fujita, Katsumasa
	NRID 1000050380093 1000050380093 researchmap permalink 7000008694 7000008694 Scopus Author ID 55628530013 55628530013 CreatorName Liu, Li
Description	Li J., Li M., Nawa Y., et al. Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue. Analytical Chemistry 96, 15765 (2024); https://doi.org/10.1021/acs.analchem.4c03871.
Abstract	I. Background: Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) have been utilized in drug toxicity evaluation, drug discovery, and treating heart failure patients, showing substantial effects. Ensuring the quality, purity, and maturation of hiPSC-CMs during large-scale production is crucial. There is a growing demand for a novel method to characterize cell molecular profiles without labels and without causing damage. II. Methods: In this study, we employed label-free Raman microscopy to evaluate hiPSC-derived CMs. The study involved the characterization of cell molecular profiles without labels and without causing damage. The correlation between Raman spectroscopy of specific components, such as cytochrome c and myoglobin, and CM purity and maturation following hiPSC differentiation was investigated. Additionally, the validation of this correlation was performed by assessing mixtures of commercially available CMs (iCell cardiomyocytes2) and fibroblasts at various ratios as well as hiPSC-derived CMs with different efficiencies. Furthermore, CMs were matured using rapid pacing of traveling waves, and the Raman profiles of matured CMs were compared to those of immature ones. III. Results: Raman spectroscopy indicated that the cytochrome c and myoglobin showed correlation with the purity and maturation of CMs following differentiation of hiPSCs. This correlation was validated through experiments involving different CM-fibroblast mixtures and hiPSC-derived CMs with varying efficiencies. Moreover, matured CMs exhibited markedly different Raman profiles compared to immature ones, indicating the potential of Raman imaging as a tool for assessing CM maturation. IV. Conclusions: We discovered that Raman spectroscopy of certain components, such as cytochrome c and myoglobin, correlates with the CM purity and maturation following hiPSC differentiation. The findings of this study highlight the potential of label-free Raman microscopy as a nondestructive, high-content, and time-efficient method for quality control of hiPSC-derived CMs. This approach could significantly contribute to ensuring the quality and maturity of hiPSC-CMs for various applications in drug discovery and regenerative medicine.
Publisher	American Chemical Society
Source Title	Analytical Chemistry
Volume (Issue)	96(39)
Page	15765-15772
Date of Issued	2024-09-18
Language	English
Handle URL	https://hdl.handle.net/11094/98346
PISSN	00032700
eISSN	15206882
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1021/acs.analchem.4c03871
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Analytical Chemistry. 2024, 96(39), p. 15765-15772
DC.title	Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue
DC.creator	Li, Junjun
	Li, Menglu
	Nawa, Yasunori
	Liu, Yuting
	Bando, Kazuki
	Hua, Ying
	Sun, Lifu
	Fujita, Satoshi
	Sawa, Yoshiki
	Fujita, Katsumasa
	Liu, Li
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-18
DC.identifier	https://doi.org/10.1021/acs.analchem.4c03871
DC.description	Li J., Li M., Nawa Y., et al. Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue. Analytical Chemistry 96, 15765 (2024); https://doi.org/10.1021/acs.analchem.4c03871.
DCTERMS.abstract	I. Background: Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) have been utilized in drug toxicity evaluation, drug discovery, and treating heart failure patients, showing substantial effects. Ensuring the quality, purity, and maturation of hiPSC-CMs during large-scale production is crucial. There is a growing demand for a novel method to characterize cell molecular profiles without labels and without causing damage. II. Methods: In this study, we employed label-free Raman microscopy to evaluate hiPSC-derived CMs. The study involved the characterization of cell molecular profiles without labels and without causing damage. The correlation between Raman spectroscopy of specific components, such as cytochrome c and myoglobin, and CM purity and maturation following hiPSC differentiation was investigated. Additionally, the validation of this correlation was performed by assessing mixtures of commercially available CMs (iCell cardiomyocytes2) and fibroblasts at various ratios as well as hiPSC-derived CMs with different efficiencies. Furthermore, CMs were matured using rapid pacing of traveling waves, and the Raman profiles of matured CMs were compared to those of immature ones. III. Results: Raman spectroscopy indicated that the cytochrome c and myoglobin showed correlation with the purity and maturation of CMs following differentiation of hiPSCs. This correlation was validated through experiments involving different CM-fibroblast mixtures and hiPSC-derived CMs with varying efficiencies. Moreover, matured CMs exhibited markedly different Raman profiles compared to immature ones, indicating the potential of Raman imaging as a tool for assessing CM maturation. IV. Conclusions: We discovered that Raman spectroscopy of certain components, such as cytochrome c and myoglobin, correlates with the CM purity and maturation following hiPSC differentiation. The findings of this study highlight the potential of label-free Raman microscopy as a nondestructive, high-content, and time-efficient method for quality control of hiPSC-derived CMs. This approach could significantly contribute to ensuring the quality and maturity of hiPSC-CMs for various applications in drug discovery and regenerative medicine.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98346
citation_title	Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue
citation_author	Li, Junjun
	Li, Menglu
	Nawa, Yasunori
	Liu, Yuting
	Bando, Kazuki
	Hua, Ying
	Sun, Lifu
	Fujita, Satoshi
	Sawa, Yoshiki
	Fujita, Katsumasa
	Liu, Li
citation_publisher	American Chemical Society
citation_language	English
citation_date	2024-09-18
citation_journal_title	Analytical Chemistry
citation_volume	96
citation_issue	39
citation_firstpage	15765
citation_lastpage	15772
citation_doi	https://doi.org/10.1021/acs.analchem.4c03871
citation_public_url	https://hdl.handle.net/11094/98346

Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue

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